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A1R6X5 (SYL_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:AAur_2246
OrganismArthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]
Taxonomic identifier290340 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length842 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABM09960.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 842842Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334728

Regions

Motif62 – 7211"HIGH" region HAMAP-Rule MF_00049
Motif607 – 6115"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6101ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1R6X5 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: FF6EE5D4F0C75C80

FASTA84293,067
        10         20         30         40         50         60 
MSVKPETETG TAQTAAAEAP EEGVYSFAAM EAKWPQVWED LKVFTPADDG SRERRYVLDM 

        70         80         90        100        110        120 
FPYPSGDLHM GHAEAFAMGD VVARYLRQKG FDVLHPIGWD SFGLPAENAA IKRNAHPSEW 

       130        140        150        160        170        180 
TYANIDTQAA SFKRYAISAD WSRRLHTSDP EYYRWTQWLF KRFYERGLAY RKDSPVNWCP 

       190        200        210        220        230        240 
KDLTVLANEQ VVNGACERCG TPVTKKSLNQ WYFKITDYAD RLLEDMDQLQ GHWPERVLAM 

       250        260        270        280        290        300 
QRNWIGRSEG AHVRFVIEAT ADRAEREVTV FTTRPDTLYG ATFFVVAADA HLALDLVTPE 

       310        320        330        340        350        360 
QHDELMAYRE KVKALSEIER QSTEREKTGV FTGRYAINPL NGEKLPVWAA DYVLADYGTG 

       370        380        390        400        410        420 
AIMAVPAHDQ RDLDFAKTFG LPVRAVLDTG DEDPAETGVA TAGEGTLKNS GELDGLSKSE 

       430        440        450        460        470        480 
GIPAAIEILE KLGTGEKFVN FRLRDWLLSR QRFWGTPIPI IHCGECGEVP VPDDQLPVRL 

       490        500        510        520        530        540 
PDDLRGEALS PKGTSPLAAA VEWVNVECPN CGRAAQRDTD TMDTFVDSSW YFLRFVSPDY 

       550        560        570        580        590        600 
TEGPFDPEKI NNWMPVGQYV GGVEHAILHL LYARFFTKVI KDIGLIEANE PFSALLNQGQ 

       610        620        630        640        650        660 
VLNGGKAMSK SLGNGVDLGE QLDKFGVDAV RLTMVFASPP EDDVDWADVS PSGSAKFLAR 

       670        680        690        700        710        720 
AWRLGQDVSS EPGVDPATGD RALRTVTHKT IADAAELLDN NKFNVVVARL MELVNATRKT 

       730        740        750        760        770        780 
IDSGAGAADP AVREAVEAVA VILSLFAPYT AEDLWNTLGH PASVANAGWP KHDDALLVQD 

       790        800        810        820        830        840 
TVTAVVQVQG KVRDRLEVSP DITEDALREL ALASENVQRA LDGRGIRTVI VRAPKLVNIV 


PA 

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References

[1]"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1."
Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J.
PLoS Genet. 2:2094-2106(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TC1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000474 Genomic DNA. Translation: ABM09960.1. Different initiation.
RefSeqYP_947987.1. NC_008711.1.

3D structure databases

ProteinModelPortalA1R6X5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290340.AAur_2246.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM09960; ABM09960; AAur_2246.
GeneID4641390.
KEGGaau:AAur_2246.
PATRIC20979912. VBIArtAur67810_2280.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycAAUR290340:GI59-2246-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ARTAT
AccessionPrimary (citable) accession number: A1R6X5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries