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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Arthrobacter aurescens (strain TC1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei610 – 6101ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAUR290340:GI59-2246-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:AAur_2246
OrganismiArthrobacter aurescens (strain TC1)
Taxonomic identifieri290340 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
ProteomesiUP000000637: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 842842Leucine--tRNA ligasePRO_0000334728Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290340.AAur_2246.

Structurei

3D structure databases

ProteinModelPortaliA1R6X5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi62 – 7211"HIGH" regionAdd
BLAST
Motifi607 – 6115"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1R6X5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVKPETETG TAQTAAAEAP EEGVYSFAAM EAKWPQVWED LKVFTPADDG
60 70 80 90 100
SRERRYVLDM FPYPSGDLHM GHAEAFAMGD VVARYLRQKG FDVLHPIGWD
110 120 130 140 150
SFGLPAENAA IKRNAHPSEW TYANIDTQAA SFKRYAISAD WSRRLHTSDP
160 170 180 190 200
EYYRWTQWLF KRFYERGLAY RKDSPVNWCP KDLTVLANEQ VVNGACERCG
210 220 230 240 250
TPVTKKSLNQ WYFKITDYAD RLLEDMDQLQ GHWPERVLAM QRNWIGRSEG
260 270 280 290 300
AHVRFVIEAT ADRAEREVTV FTTRPDTLYG ATFFVVAADA HLALDLVTPE
310 320 330 340 350
QHDELMAYRE KVKALSEIER QSTEREKTGV FTGRYAINPL NGEKLPVWAA
360 370 380 390 400
DYVLADYGTG AIMAVPAHDQ RDLDFAKTFG LPVRAVLDTG DEDPAETGVA
410 420 430 440 450
TAGEGTLKNS GELDGLSKSE GIPAAIEILE KLGTGEKFVN FRLRDWLLSR
460 470 480 490 500
QRFWGTPIPI IHCGECGEVP VPDDQLPVRL PDDLRGEALS PKGTSPLAAA
510 520 530 540 550
VEWVNVECPN CGRAAQRDTD TMDTFVDSSW YFLRFVSPDY TEGPFDPEKI
560 570 580 590 600
NNWMPVGQYV GGVEHAILHL LYARFFTKVI KDIGLIEANE PFSALLNQGQ
610 620 630 640 650
VLNGGKAMSK SLGNGVDLGE QLDKFGVDAV RLTMVFASPP EDDVDWADVS
660 670 680 690 700
PSGSAKFLAR AWRLGQDVSS EPGVDPATGD RALRTVTHKT IADAAELLDN
710 720 730 740 750
NKFNVVVARL MELVNATRKT IDSGAGAADP AVREAVEAVA VILSLFAPYT
760 770 780 790 800
AEDLWNTLGH PASVANAGWP KHDDALLVQD TVTAVVQVQG KVRDRLEVSP
810 820 830 840
DITEDALREL ALASENVQRA LDGRGIRTVI VRAPKLVNIV PA
Length:842
Mass (Da):93,067
Last modified:May 20, 2008 - v2
Checksum:iFF6EE5D4F0C75C80
GO

Sequence cautioni

The sequence ABM09960.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000474 Genomic DNA. Translation: ABM09960.1. Different initiation.
RefSeqiYP_947987.1. NC_008711.1.

Genome annotation databases

EnsemblBacteriaiABM09960; ABM09960; AAur_2246.
GeneIDi4641390.
KEGGiaau:AAur_2246.
PATRICi20979912. VBIArtAur67810_2280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000474 Genomic DNA. Translation: ABM09960.1. Different initiation.
RefSeqiYP_947987.1. NC_008711.1.

3D structure databases

ProteinModelPortaliA1R6X5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290340.AAur_2246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM09960; ABM09960; AAur_2246.
GeneIDi4641390.
KEGGiaau:AAur_2246.
PATRICi20979912. VBIArtAur67810_2280.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciAAUR290340:GI59-2246-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TC1.

Entry informationi

Entry nameiSYL_ARTAT
AccessioniPrimary (citable) accession number: A1R6X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.