A1R5H8 (PANB_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase
EC=2.1.2.11

Alternative name(s):
Ketopantoate hydroxymethyltransferase
Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	AAur_1730

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the PanB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_0000297216
Regions
	Region	82 – 83	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	219	1	Proton acceptor By similarity
	Metal binding	82	1	Magnesium By similarity
	Metal binding	121	1	Magnesium By similarity
	Metal binding	153	1	Magnesium By similarity
	Binding site	121	1	Alpha-ketoisovalerate By similarity
	Binding site	151	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1R5H8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: EA2F108E44BDA694	FASTA	301	31,540
	10 20 30 40 50 60 MAPSNLPEST TPAEVPAPYG TGPAAAAQAA SAAGRKPISR VRIHHLQQAK DNGEHFAMLT 70 80 90 100 110 120 AYEQYTAEIF DQAGIEVLLV GDSASNNVYG NETSLPVTVD ELLPLTRAVS RSAKRALIVA 130 140 150 160 170 180 DLPFGSYEVS PGQAVATGVR FLKEGLAHAV KIEGTAYYAD TVRAMVQAGI PVMAHIGFTP 190 200 210 220 230 240 QSEHSLGGYR VQGRGDDAQR LVDDAVALQD AGAFSVLMEM VPAETAAAVD AALRVPTVGI 250 260 270 280 290 300 GAGKTTTGQV LVWQDMAGLR GGKMAKFVKQ YADLRTTLSD AAAAYGEDVR SGQFPGPEHS F

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TC1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000474 Genomic DNA. Translation: ABM08191.1.
RefSeq	YP_947490.1. NC_008711.1.
3D structure databases
ProteinModelPortal	A1R5H8.
SMR	A1R5H8. Positions 39-299.
ModBase	Search...
Protein-protein interaction databases
STRING	A1R5H8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4638844.
GenomeReviews	Gene locus AAur_1730 in contig CP000474_GR.
KEGG	aau:AAur_1730.
PATRIC	20978860. VBIArtAur67810_1763.
TIGR	AAur_1730.
Phylogenomic databases
eggNOG	COG0413.
HOGENOM	HBG299908.
OMA	QVLVWTD.
ProtClustDB	PRK00311.
Enzyme and pathway databases
BioCyc	AAUR290340:AAUR_1730-MONOMER.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K00606.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR00222. PanB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_ARTAT

Accession

Primary (citable) accession number: A1R5H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program