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Protein

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Gene

hisA

Organism
Arthrobacter aurescens (strain TC1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi: L-histidine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Phosphoribosyl-ATP pyrophosphatase (hisE)
  3. Phosphoribosyl-AMP cyclohydrolase (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
  6. Imidazoleglycerol-phosphate dehydratase (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. no protein annotated in this organism
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei16Proton acceptorUniRule annotation1
Active sitei135Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00031; UER00009.

Names & Taxonomyi

Protein namesi
Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomeraseUniRule annotation (EC:5.3.1.16UniRule annotation)
Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomeraseUniRule annotation
Gene namesi
Name:hisAUniRule annotation
Ordered Locus Names:AAur_1608
OrganismiArthrobacter aurescens (strain TC1)
Taxonomic identifieri290340 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaePaenarthrobacter
Proteomesi
  • UP000000637 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002904471 – 2471-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomeraseAdd BLAST247

Interactioni

Protein-protein interaction databases

STRINGi290340.AAur_1608.

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 18Combined sources9
Beta strandi21 – 25Combined sources5
Beta strandi34 – 37Combined sources4
Helixi39 – 48Combined sources10
Beta strandi52 – 58Combined sources7
Helixi59 – 62Combined sources4
Helixi69 – 78Combined sources10
Beta strandi80 – 88Combined sources9
Helixi92 – 100Combined sources9
Beta strandi104 – 108Combined sources5
Helixi110 – 114Combined sources5
Helixi116 – 126Combined sources11
Helixi127 – 129Combined sources3
Beta strandi130 – 137Combined sources8
Helixi153 – 163Combined sources11
Beta strandi168 – 172Combined sources5
Helixi184 – 194Combined sources11
Beta strandi198 – 202Combined sources5
Helixi207 – 214Combined sources8
Helixi215 – 219Combined sources5
Beta strandi221 – 226Combined sources6
Helixi228 – 231Combined sources4
Helixi237 – 244Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WD0X-ray1.50A1-247[»]
ProteinModelPortaliA1R562.
SMRiA1R562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CJV. Bacteria.
COG0106. LUCA.
HOGENOMiHOG000224614.
KOiK01814.
OMAiEWLHLVD.
OrthoDBiPOG091H048O.

Family and domain databases

CDDicd04732. HisA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01014. HisA. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR010188. HisA_TrpF.
IPR023016. Isoase_HisA.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00977. His_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01919. hisA-trpF. 1 hit.

Sequencei

Sequence statusi: Complete.

A1R562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSAQSVLE LLPAVDIVDG QAVRLLQGEA GSETSYGTPL EAALNWQNDG
60 70 80 90 100
AEWVHMVDLD AAFGRGNNAA LISDVVSQLN VKVELSGGLR DDESLERALE
110 120 130 140 150
LGVARVNLGT AALENPEWTR KAIDRFGDKI AVGLDVRGTT LAGRGWTKEG
160 170 180 190 200
GDLWEVLARL EDAGCARYVV TDVTKDGTLQ GPNVELLRQM VEKTGKPVVA
210 220 230 240
SGGISSLEDL RVLRELVPLG VEGAIVGKAL YAGAFTLPEA LDVAGRR
Length:247
Mass (Da):26,175
Last modified:February 6, 2007 - v1
Checksum:iA0839617E7505388
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000474 Genomic DNA. Translation: ABM09313.1.
RefSeqiWP_011774319.1. NC_008711.1.

Genome annotation databases

EnsemblBacteriaiABM09313; ABM09313; AAur_1608.
KEGGiaau:AAur_1608.
PATRICi20978606. VBIArtAur67810_1640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000474 Genomic DNA. Translation: ABM09313.1.
RefSeqiWP_011774319.1. NC_008711.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WD0X-ray1.50A1-247[»]
ProteinModelPortaliA1R562.
SMRiA1R562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290340.AAur_1608.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM09313; ABM09313; AAur_1608.
KEGGiaau:AAur_1608.
PATRICi20978606. VBIArtAur67810_1640.

Phylogenomic databases

eggNOGiENOG4105CJV. Bacteria.
COG0106. LUCA.
HOGENOMiHOG000224614.
KOiK01814.
OMAiEWLHLVD.
OrthoDBiPOG091H048O.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00009.

Family and domain databases

CDDicd04732. HisA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01014. HisA. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR010188. HisA_TrpF.
IPR023016. Isoase_HisA.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00977. His_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01919. hisA-trpF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHIS4_ARTAT
AccessioniPrimary (citable) accession number: A1R562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.