A1R556 (LEXA_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LexA repressor
EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	AAur_1602

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	LexA repressor HAMAP MF_00015		PRO_0000322713
Regions
	DNA binding	48 – 68	21	H-T-H motif By similarity
Sites
	Active site	171	1	For autocatalytic cleavage activity By similarity
	Active site	208	1	For autocatalytic cleavage activity By similarity
	Site	136 – 137	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1R556 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 36BC0C23BDD2425A	FASTA	247	26,322
	10 20 30 40 50 60 MAAKATGGGA PLRSQQPQKS PKSLTVRQKK ILETIQRSVN DNGYPPSMRE IGDTVGLASL 70 80 90 100 110 120 SSVTHQLSQL EKLGYLRRDP KRPRAMEVLM PLTLDGGAIP GVEAPTTLRS AGGLAVTELA 130 140 150 160 170 180 SASDTAMVPL VGRIAAGGPI LADQTVEDVL ALPRQLVGHG ELFMLKVAGD SMIDAAICDG 190 200 210 220 230 240 DWVVVRRQND AINGDIVAAL LDDEATVKTF RQRDGHTWLL PQNTQYEPIL GDQATIMGKV VSVLRSL

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TC1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000474 Genomic DNA. Translation: ABM08664.1.
RefSeq	YP_947368.1. NC_008711.1.
3D structure databases
ProteinModelPortal	A1R556.
ModBase	Search...
Protein-protein interaction databases
STRING	A1R556.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4640849.
GenomeReviews	Gene locus AAur_1602 in contig CP000474_GR.
KEGG	aau:AAur_1602.
PATRIC	20978594. VBIArtAur67810_1634.
TIGR	AAur_1602.
Phylogenomic databases
eggNOG	COG1974.
HOGENOM	HBG679610.
OMA	IAENPDF.
ProtClustDB	PRK00215.
Enzyme and pathway databases
BioCyc	AAUR290340:AAUR_1602-MONOMER.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01356.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR00498. LexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_ARTAT

Accession

Primary (citable) accession number: A1R556

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program