Reviewed, UniProtKB/Swiss-Prot A1R508 (SYP_ARTAT)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	AAur_1553

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 603	603	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000288309

Sequences

	Sequence		Length	Mass (Da)
	A1R508-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 44AE124E8F3AC60B	FASTA	603	65,242
	10 20 30 40 50 60 MVLRLSQLFL RTLREDPVDA EVDSHKLLVR AGYIRRAAPG IYTWLPLGLS VLRKVEDIIR 70 80 90 100 110 120 QEMSAIGAQE VHFPALLPRE PYEATNRWTE YGEGLFRLQD RKGADYLLAP THEEMFTLLV 130 140 150 160 170 180 KDLYSSYKDL PLSLYQIQNK YRDEARPRAG LLRGREFIMK DSYSFDIDDA GLDASYAAHR 190 200 210 220 230 240 AAYLKIFERL GLEVIPVAAT AGAMGGSKSE EFLFPTEIGE DTFVRSAGGY YANVEAVTTV 250 260 270 280 290 300 VPDEIDFSNA PAAEVLDTPN TPTIDTLVDA ANQLAPRSES DGGAWTAADT LKNVVLAVTL 310 320 330 340 350 360 PTGERQIVVI GVPGDRGVDL KRVEANIGSH LPIAGEIGLE AANEEDLKKL PWLVKGYIGP 370 380 390 400 410 420 GLSLDEPVLG LEGSSKVLFL VDPRVVSGTT WVTGANAEGK HVFGLVAGRD FVWDGVIEST 430 440 450 460 470 480 EVRAGDPAPD GSGPLETARG IEMGHIFQLG RKYAEALDLK VLDQNGKQQV VTMGSYGVGV 490 500 510 520 530 540 TRAVAALAEA NNDDRGLVWP RSVAPADVHV VAVGRGDEIF EAAEKLSLEL EAAGLDVIFD 550 560 570 580 590 600 DRPKVSPGVK FGDAELVGVP TILAVGRGLV DGVVEIKDRR SGEAENISVD KAVDYVVNAV RTR

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000474 Genomic DNA. Translation: ABM09437.1.
RefSeq	YP_947320.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1R508.
Genome annotation databases
GeneID	4640938.
GenomeReviews	Gene locus AAur_1553 in contig CP000474_GR.
KEGG	aau:AAur_1553.
TIGR	AAur_1553.
Phylogenomic databases
OMA	VVSHQLM.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ARTAT

Accession

Primary (citable) accession number: A1R508

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)