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A1R4P6 (DNLJ_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Ordered Locus Names:AAur_1434
OrganismArthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]
Taxonomic identifier290340 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length768 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 768768DNA ligase HAMAP MF_01588
PRO_0000313116

Regions

Domain661 – 75090BRCT
Nucleotide binding61 – 655NAD By similarity
Nucleotide binding110 – 1112NAD By similarity

Sites

Active site1481N6-AMP-lysine intermediate By similarity
Metal binding4431Zinc By similarity
Metal binding4461Zinc By similarity
Metal binding4621Zinc By similarity
Metal binding4681Zinc By similarity
Binding site1461NAD By similarity
Binding site1691NAD By similarity
Binding site2061NAD By similarity
Binding site3221NAD By similarity
Binding site3461NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A1R4P6 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 7F92736417DC2050

FASTA76883,412
        10         20         30         40         50         60 
MSTPENPDPV DTTTQDAAAA IVAEEGTPPS ESLRDEYEQL ADLVRKYRYA YYQEDSPTVS 

        70         80         90        100        110        120 
DAEFDELYRR LEELEALHPE LVSNDSPTQE VGGEVSSAFA AVEHLQRMYS LDDVFSLDEL 

       130        140        150        160        170        180 
EAWVRKAEAS VAKLGDSVPP IAWLTELKID GLAVNLLYRD GKLVRAATRG DGTTGEDITH 

       190        200        210        220        230        240 
NVLTIKEIPR QLSGSGYPSE VEIRGEVFIP SKAFLEFNES LVAAGKAPLA NPRNAAAGSL 

       250        260        270        280        290        300 
RQKDPAETAK RPLSMFVHGI GAREGLDAKS QSESYKLLEE WGLPVSPYLK VLETFDDVLK 

       310        320        330        340        350        360 
FIADYGERRH KLVHEIDGIV VKIDDFATQR ALGYTSRVPR WAAAYKYPPE EVHTKLLDIA 

       370        380        390        400        410        420 
VNVGRTGRVT PFGLMEPVKV AGSTVGMATL HNQDVVKAKG VMIGDIVILR KAGDVIPEIV 

       430        440        450        460        470        480 
GPVLALRDKQ EPPVREFVMP TECPSCGTPL APAKESDVDI RCPNAKSCPS QLRERVFHVA 

       490        500        510        520        530        540 
SRGAFDIEAL GWEAAVALTQ PAEPETPPLT SEARLFSLTR EDLADVLIRR EKRSKGVGTG 

       550        560        570        580        590        600 
EYELVPYFYT KGTAKSPSKP TATTEKLFAE LEKAKQQPLW RVLVALSIRH VGPTASRALA 

       610        620        630        640        650        660 
TAFGSMDAIR NATEEQMAHV DGVGPTIAVA LKEWFAVDWH NEIVDSWAAA GVRMEDERDA 

       670        680        690        700        710        720 
SVPRTLEGLT VVVTGTLPNF SRDEAKEAII IRGGKASGSV SKNTSYLVAG ESAGTKLDKA 

       730        740        750        760 
EQLGVPVLDE DGFRELLANG PAQTGTEAEA ATDEATVVDE TAAEAATE

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References

[1]"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1."
Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J.
PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TC1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000474 Genomic DNA. Translation: ABM07125.1.
RefSeqYP_947208.1. NC_008711.1.

3D structure databases

ProteinModelPortalA1R4P6.
SMRA1R4P6. Positions 32-350.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1R4P6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4639882.
GenomeReviewsGene locus AAur_1434 in contig CP000474_GR.
KEGGaau:AAur_1434.
PATRIC20978248. VBIArtAur67810_1464.
TIGRAAur_1434.

Phylogenomic databases

eggNOGCOG0272.
HOGENOMHBG620317.
OMAENVRTIR.
ProtClustDBCLSK959647.

Enzyme and pathway databases

BioCycAAUR290340:AAUR_1434-MONOMER.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_ARTAT
AccessionPrimary (citable) accession number: A1R4P6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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