Reviewed, UniProtKB/Swiss-Prot A1R4F8 (ISPE_ARTAT)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	AAur_1338

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335698
Regions
	Nucleotide binding	109 – 119	11	ATP Potential
Sites
	Active site	17	1	By similarity
	Active site	151	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1R4F8-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: E3618581CFDA8EB6	FASTA	317	32,831
	10 20 30 40 50 60 MGERFHARTV RVKAPGKVNV SLSVGPLRPD GYHSVASVYL AVSLYEEVAA TSTEAPGITV 70 80 90 100 110 120 SISPDSTLDL DGVDIPLDQR NLAYKAAAIM AEMSEKPTGV HLEITKRVPV AGGMGGGSAD 130 140 150 160 170 180 AAATLLACDA LWNSGLSREE LAHLAAELGA DVPFSLLGGT AVGLGVGDKL SPALAKAQMD 190 200 210 220 230 240 WVLVFADYGL STPDVFRTLD GLRDSEGVEI PEPVDVDPTI LQALRKGDPE TLSRVLINDL 250 260 270 280 290 300 QRASITLAPQ LRDTIGLGEA RGALAGMVSG SGPTIALLAR DSVSASVLAE ELTHRGHNAL 310 AVHGPVPGAR IISDTLL

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000474 Genomic DNA. Translation: ABM06709.1.
RefSeq	YP_947120.1.
3D structure databases
SMR	A1R4F8. Positions 10-294.
ModBase	Search...
Protein-protein interaction databases
STRING	A1R4F8.
Genome annotation databases
GeneID	4638199.
GenomeReviews	Gene locus AAur_1338 in contig CP000474_GR.
KEGG	aau:AAur_1338.
TIGR	AAur_1338.
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	GVHLEIT.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_ARTAT

Accession

Primary (citable) accession number: A1R4F8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)