Reviewed, UniProtKB/Swiss-Prot A1R451 (FOLD2_ARTAT)

Names and origin

Protein names

Recommended name:
    Bifunctional protein folD 2
Including the following 2 domains:
    1- Recommended name:
            Methylenetetrahydrofolate dehydrogenase
              EC=1.5.1.5
    2- Recommended name:
            Methenyltetrahydrofolate cyclohydrolase
              EC=3.5.4.9

Gene names

Name:	folD2
Ordered Locus Names:	AAur_1228

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. HAMAP MF_01576 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. HAMAP MF_01576
Pathway	One-carbon metabolism; tetrahydrofolate pathway. HAMAP MF_01576
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis Methionine biosynthesis One-carbon metabolism Purine biosynthesis
Ligand	NADP
Molecular function	Hydrolase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Bifunctional protein folD 2 HAMAP MF_01576		PRO_0000305791

Sequences

	Sequence		Length	Mass (Da)
	A1R451-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 36A4821A4F5672EB	FASTA	297	31,093
	10 20 30 40 50 60 MTQSTAQILD GKATAAAIKA ELTTRVSVLA AKGIVPGLGT ILVGSDPGST WYVGGKHKDC 70 80 90 100 110 120 AEVGIQSIRR DLPEDISQED LLEVVRELND NPECTGYIVQ LPLPKHIDQD VILEAMDPDK 130 140 150 160 170 180 DADGLHPMNL GRLVANVNGE MKSPLPCTPK GCVELLRRHN IELKGKRVLV VGRGVTIGRP 190 200 210 220 230 240 IGLLLTRKEV NATVILAHTG TVDLPAELKQ ADVVIAAAGV PHMIKAEDLK PGAVVLDVGV 250 260 270 280 290 SRVDDGNGKA VVTGDVDPAA ADVAAWLSPN PGGVGPMTRA MLLANVVESA ERQAGIA

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000474 Genomic DNA. Translation: ABM06658.1.
RefSeq	YP_947013.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4639361.
GenomeReviews	Gene locus AAur_1228 in contig CP000474_GR.
KEGG	aau:AAur_1228.
TIGR	AAur_1228.
Phylogenomic databases
OMA	A1R451. VDLKQRV.
Family and domain databases
HAMAP	MF_01576. [Tree]
InterPro	IPR000672. THF_DH/CycHdrlase. [Graphical view]
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
ProDom	PD002300. THFDhg/Cyc_hydro. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00766. THF_DHG_CYH_1. False negative. PS00767. THF_DHG_CYH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLD2_ARTAT

Accession

Primary (citable) accession number: A1R451

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)