A1R451 (FOLD2_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein FolD 2

Including the following 2 domains:

1. Methylenetetrahydrofolate dehydrogenase
   EC=1.5.1.5
2. Methenyltetrahydrofolate cyclohydrolase
   EC=3.5.4.9

Gene names

Name:	folD2
Ordered Locus Names:	AAur_1228

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity. HAMAP MF_01576
Catalytic activity	5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. HAMAP MF_01576 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. HAMAP MF_01576
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01576
Subunit structure	Homodimer By similarity. HAMAP MF_01576
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis Methionine biosynthesis One-carbon metabolism Purine biosynthesis
Ligand	NADP
Molecular function	Hydrolase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: EC methylenetetrahydrofolate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Bifunctional protein FolD 2 HAMAP MF_01576		PRO_0000305791

Sequences

	Sequence		Length	Mass (Da)
	A1R451 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 36A4821A4F5672EB	FASTA	297	31,093
	10 20 30 40 50 60 MTQSTAQILD GKATAAAIKA ELTTRVSVLA AKGIVPGLGT ILVGSDPGST WYVGGKHKDC 70 80 90 100 110 120 AEVGIQSIRR DLPEDISQED LLEVVRELND NPECTGYIVQ LPLPKHIDQD VILEAMDPDK 130 140 150 160 170 180 DADGLHPMNL GRLVANVNGE MKSPLPCTPK GCVELLRRHN IELKGKRVLV VGRGVTIGRP 190 200 210 220 230 240 IGLLLTRKEV NATVILAHTG TVDLPAELKQ ADVVIAAAGV PHMIKAEDLK PGAVVLDVGV 250 260 270 280 290 SRVDDGNGKA VVTGDVDPAA ADVAAWLSPN PGGVGPMTRA MLLANVVESA ERQAGIA

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TC1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000474 Genomic DNA. Translation: ABM06658.1.
RefSeq	YP_947013.1. NC_008711.1.
3D structure databases
ProteinModelPortal	A1R451.
SMR	A1R451. Positions 6-293.
ModBase	Search...
Protein-protein interaction databases
STRING	A1R451.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4639361.
GenomeReviews	Gene locus AAur_1228 in contig CP000474_GR.
KEGG	aau:AAur_1228.
PATRIC	20977808. VBIArtAur67810_1246.
TIGR	AAur_1228.
Phylogenomic databases
eggNOG	COG0190.
HOGENOM	HBG328751.
OMA	LLMKENA.
ProtClustDB	CLSK2504874.
Enzyme and pathway databases
BioCyc	AAUR290340:AAUR_1228-MONOMER.
Family and domain databases
HAMAP	MF_01576. THF_DHG_CYH. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. IPR020630. THF_DH/CycHdrlase_cat_dom. IPR020631. THF_DH/CycHdrlase_NAD-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01491.
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
PROSITE	PS00766. THF_DHG_CYH_1. False negative. PS00767. THF_DHG_CYH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLD2_ARTAT

Accession

Primary (citable) accession number: A1R451

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program