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A1R3R7

- BIOB_ARTAT

UniProt

A1R3R7 - BIOB_ARTAT

Protein

Biotin synthase

Gene

bioB

Organism
Arthrobacter aurescens (strain TC1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi134 – 1341Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi167 – 1671Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi226 – 2261Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi296 – 2961Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciAAUR290340:GI59-1091-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:AAur_1091
    OrganismiArthrobacter aurescens (strain TC1)
    Taxonomic identifieri290340 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
    ProteomesiUP000000637: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 355355Biotin synthasePRO_0000381208Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi290340.AAur_1091.

    Structurei

    3D structure databases

    ProteinModelPortaliA1R3R7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiTCENTLR.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A1R3R7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVTSTDTVK DMTTRATQAP RETASYAILE TAREQVLERG EGLNESQLIH    50
    ILELPDDAIP EALQLAHEVR LKHCGEDVEV EGIISIKTGG CPEDCHFCSQ 100
    SGLFDSPVRG VWLDIPELVK AAKETAATGA TEFCIVAAVR GPDIKLMNQI 150
    KFAIDRINEE VDINIACSLG MLTQRQVDQL AEWGVHRYNH NLETARSYFP 200
    EVVTTHSYEE RLETCAMVKA AGMELCCGAL IGMGESLAQR AELAAQLAAL 250
    EPHEVPLNFL NPRPGTPLEN QGIMDGKDAL RAIAAFRLAM PRTVLRYAGG 300
    RELTLGDLGT REGLLGGINA VIVGNYLTTL GRPANADLNL LVELNMPIKE 350
    FQKSL 355
    Length:355
    Mass (Da):38,698
    Last modified:February 6, 2007 - v1
    Checksum:iD49A25314769B14D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000474 Genomic DNA. Translation: ABM09820.1.
    RefSeqiYP_946879.1. NC_008711.1.

    Genome annotation databases

    EnsemblBacteriaiABM09820; ABM09820; AAur_1091.
    GeneIDi4638223.
    KEGGiaau:AAur_1091.
    PATRICi20977532. VBIArtAur67810_1108.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000474 Genomic DNA. Translation: ABM09820.1 .
    RefSeqi YP_946879.1. NC_008711.1.

    3D structure databases

    ProteinModelPortali A1R3R7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290340.AAur_1091.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM09820 ; ABM09820 ; AAur_1091 .
    GeneIDi 4638223.
    KEGGi aau:AAur_1091.
    PATRICi 20977532. VBIArtAur67810_1108.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi TCENTLR.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci AAUR290340:GI59-1091-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TC1.

    Entry informationi

    Entry nameiBIOB_ARTAT
    AccessioniPrimary (citable) accession number: A1R3R7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3