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A1R3R7

- BIOB_ARTAT

UniProt

A1R3R7 - BIOB_ARTAT

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Protein
Biotin synthase
Gene
bioB, AAur_1091
Organism
Arthrobacter aurescens (strain TC1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi134 – 1341Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi167 – 1671Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi226 – 2261Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi296 – 2961Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAAUR290340:GI59-1091-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:AAur_1091
OrganismiArthrobacter aurescens (strain TC1)
Taxonomic identifieri290340 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
ProteomesiUP000000637: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Biotin synthaseUniRule annotation
PRO_0000381208Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi290340.AAur_1091.

Structurei

3D structure databases

ProteinModelPortaliA1R3R7.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1R3R7-1 [UniParc]FASTAAdd to Basket

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MPVTSTDTVK DMTTRATQAP RETASYAILE TAREQVLERG EGLNESQLIH    50
ILELPDDAIP EALQLAHEVR LKHCGEDVEV EGIISIKTGG CPEDCHFCSQ 100
SGLFDSPVRG VWLDIPELVK AAKETAATGA TEFCIVAAVR GPDIKLMNQI 150
KFAIDRINEE VDINIACSLG MLTQRQVDQL AEWGVHRYNH NLETARSYFP 200
EVVTTHSYEE RLETCAMVKA AGMELCCGAL IGMGESLAQR AELAAQLAAL 250
EPHEVPLNFL NPRPGTPLEN QGIMDGKDAL RAIAAFRLAM PRTVLRYAGG 300
RELTLGDLGT REGLLGGINA VIVGNYLTTL GRPANADLNL LVELNMPIKE 350
FQKSL 355
Length:355
Mass (Da):38,698
Last modified:February 6, 2007 - v1
Checksum:iD49A25314769B14D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000474 Genomic DNA. Translation: ABM09820.1.
RefSeqiYP_946879.1. NC_008711.1.

Genome annotation databases

EnsemblBacteriaiABM09820; ABM09820; AAur_1091.
GeneIDi4638223.
KEGGiaau:AAur_1091.
PATRICi20977532. VBIArtAur67810_1108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000474 Genomic DNA. Translation: ABM09820.1 .
RefSeqi YP_946879.1. NC_008711.1.

3D structure databases

ProteinModelPortali A1R3R7.
ModBasei Search...

Protein-protein interaction databases

STRINGi 290340.AAur_1091.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM09820 ; ABM09820 ; AAur_1091 .
GeneIDi 4638223.
KEGGi aau:AAur_1091.
PATRICi 20977532. VBIArtAur67810_1108.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci AAUR290340:GI59-1091-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TC1.

Entry informationi

Entry nameiBIOB_ARTAT
AccessioniPrimary (citable) accession number: A1R3R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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