Reviewed, UniProtKB/Swiss-Prot A1R383 (SYC_ARTAT)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	AAur_0901

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_1000006561
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	283 – 287	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	227	1	Zinc By similarity
	Metal binding	252	1	Zinc By similarity
	Metal binding	256	1	Zinc By similarity
	Binding site	286	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1R383-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: CD450A30D871D1C9	FASTA	484	53,364
	10 20 30 40 50 60 MTLRFYDTAS AEVRDFVPLE DGKASVYYCG ATVQGMPHVG HVRSAIAFDQ LTRWLEFRGL 70 80 90 100 110 120 RVTVVRNVTD IDDKILAKSA QSFGPDWDAE PSARQTEEWW ALAYRYEQEF ENAYESLGVQ 130 140 150 160 170 180 RPTYEPRATG HIPEMHTLIQ RLIDRGHAYP ALDDSGDVYF DVRSWSKYGS LTRQNIDDMQ 190 200 210 220 230 240 GAPDADPRGK RDPRDFALWK GFKDGEPVTA KWESPWGAGR PGWHLECSAM VTKYLGPRFD 250 260 270 280 290 300 IHGGGLDLRF PHHENEMAQS QAAGDEFANF WMHNGMVTYE GEKMSKSIGN TVSPAEMLEL 310 320 330 340 350 360 ASPRVVRYYL GQAHYRSILD YRPTSLQEAA AAVERIDGFI HKASSKVGTG APDVSPQANM 370 380 390 400 410 420 PAAFIAAMDD DLNVPQALGV LHETVRAGNT ALASGDLEGA KAALYSVLSM TEVLGLDSVK 430 440 450 460 470 480 RPEAVQGREH AALEVLIEAQ LEARAAARAN KDWAASDAIR DTLAAAGVVV EDGADGATWS LKRD

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000474 Genomic DNA. Translation: ABM06615.1.
RefSeq	YP_946695.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1R383.
Genome annotation databases
GeneID	4638719.
GenomeReviews	Gene locus AAur_0901 in contig CP000474_GR.
KEGG	aau:AAur_0901.
TIGR	AAur_0901.
Phylogenomic databases
OMA	VLWKAAK.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ARTAT

Accession

Primary (citable) accession number: A1R383

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)