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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Arthrobacter aurescens (strain TC1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821SubstrateUniRule annotation
Binding sitei224 – 2241Substrate; via amide nitrogenUniRule annotation
Metal bindingi231 – 2311ManganeseUniRule annotation
Binding sitei231 – 2311SubstrateUniRule annotation
Metal bindingi251 – 2511Manganese; via tele nitrogenUniRule annotation
Binding sitei273 – 2731SubstrateUniRule annotation
Active sitei275 – 2751UniRule annotation
Metal bindingi298 – 2981ManganeseUniRule annotation
Binding sitei391 – 3911GTPUniRule annotation
Binding sitei422 – 4221GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi274 – 2796GTPUniRule annotation
Nucleotide bindingi517 – 5204GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAUR290340:GI59-832-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:AAur_0832
OrganismiArthrobacter aurescens (strain TC1)
Taxonomic identifieri290340 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
ProteomesiUP000000637: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Phosphoenolpyruvate carboxykinase [GTP]PRO_1000072369Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi290340.AAur_0832.

Structurei

3D structure databases

ProteinModelPortaliA1R317.
SMRiA1R317. Positions 13-608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni389 – 3913Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1R317-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDLARLPLL EKAPTTHARL LAWVEEVAEL TQPDRIHWVD GSEAENKKLT
60 70 80 90 100
DELVEAGTLK RLNPETFPNS FAAFSDPADV ARVEEQTFIC SENERDAGFT
110 120 130 140 150
NNWMAPAEMK QKLRGLFAGS MRGRTMYVIP FVMGHLDAED PKFGVEITDS
160 170 180 190 200
AYVVASMRIM ARIGTDVLDR ITQTDAFFVP ALHSLGAPLE AGQADVAWPC
210 220 230 240 250
NTDKWIVHFP EERSIWSFGS GYGGNALLGK KCYALRIASV MARDEGWLAE
260 270 280 290 300
HMLILKLTSP EQKTYYISAA FPSACGKTNL ALLDPTIKGW KVETLGDDIT
310 320 330 340 350
WMRFGKEGEL RAVNPEAGLF GVAPGTGWGT NPNAMRAIAK GNSIFTNVAL
360 370 380 390 400
TDDGGVWWEG MTEETPAHLT DWRGESWTPD SDAPAAHPNS RFCTPIDQID
410 420 430 440 450
MLAEEYFSPE GVELSAILFG GRRKTTIPLV TEARNWSNGI FMGSTLSSET
460 470 480 490 500
TAAAAGAVGV VRRDPMAMLP FIGYDAGDYL NHWVNLSAKA NPERLPKIFL
510 520 530 540 550
VNWFRRTAEG GFAWPGFGDN ARVLKWAIER LEGKADAVET PIGFVPTGES
560 570 580 590 600
IDLEGLDMTP AEVESAVRVD PAEWATELAS IEEWFANFGE SLPAALQSEL

DGLKTRLG
Length:608
Mass (Da):66,672
Last modified:February 6, 2007 - v1
Checksum:i8CD348EF6CF1A564
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000474 Genomic DNA. Translation: ABM06961.1.
RefSeqiYP_946629.1. NC_008711.1.

Genome annotation databases

EnsemblBacteriaiABM06961; ABM06961; AAur_0832.
GeneIDi4641427.
KEGGiaau:AAur_0832.
PATRICi20976990. VBIArtAur67810_0845.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000474 Genomic DNA. Translation: ABM06961.1.
RefSeqiYP_946629.1. NC_008711.1.

3D structure databases

ProteinModelPortaliA1R317.
SMRiA1R317. Positions 13-608.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290340.AAur_0832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM06961; ABM06961; AAur_0832.
GeneIDi4641427.
KEGGiaau:AAur_0832.
PATRICi20976990. VBIArtAur67810_0845.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciAAUR290340:GI59-832-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TC1.

Entry informationi

Entry nameiPCKG_ARTAT
AccessioniPrimary (citable) accession number: A1R317
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 6, 2007
Last modified: January 7, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.