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A1R317 (PCKG_ARTAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:AAur_0832
OrganismArthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]
Taxonomic identifier290340 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 608608Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000072369

Regions

Nucleotide binding274 – 2796GTP By similarity
Nucleotide binding517 – 5204GTP By similarity
Region389 – 3913Substrate binding By similarity

Sites

Active site2751 By similarity
Metal binding2311Manganese By similarity
Metal binding2511Manganese; via tele nitrogen By similarity
Metal binding2981Manganese By similarity
Binding site821Substrate By similarity
Binding site2241Substrate; via amide nitrogen By similarity
Binding site2311Substrate By similarity
Binding site2731Substrate By similarity
Binding site3911GTP By similarity
Binding site4221GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1R317 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 8CD348EF6CF1A564

FASTA60866,672
        10         20         30         40         50         60 
MGDLARLPLL EKAPTTHARL LAWVEEVAEL TQPDRIHWVD GSEAENKKLT DELVEAGTLK 

        70         80         90        100        110        120 
RLNPETFPNS FAAFSDPADV ARVEEQTFIC SENERDAGFT NNWMAPAEMK QKLRGLFAGS 

       130        140        150        160        170        180 
MRGRTMYVIP FVMGHLDAED PKFGVEITDS AYVVASMRIM ARIGTDVLDR ITQTDAFFVP 

       190        200        210        220        230        240 
ALHSLGAPLE AGQADVAWPC NTDKWIVHFP EERSIWSFGS GYGGNALLGK KCYALRIASV 

       250        260        270        280        290        300 
MARDEGWLAE HMLILKLTSP EQKTYYISAA FPSACGKTNL ALLDPTIKGW KVETLGDDIT 

       310        320        330        340        350        360 
WMRFGKEGEL RAVNPEAGLF GVAPGTGWGT NPNAMRAIAK GNSIFTNVAL TDDGGVWWEG 

       370        380        390        400        410        420 
MTEETPAHLT DWRGESWTPD SDAPAAHPNS RFCTPIDQID MLAEEYFSPE GVELSAILFG 

       430        440        450        460        470        480 
GRRKTTIPLV TEARNWSNGI FMGSTLSSET TAAAAGAVGV VRRDPMAMLP FIGYDAGDYL 

       490        500        510        520        530        540 
NHWVNLSAKA NPERLPKIFL VNWFRRTAEG GFAWPGFGDN ARVLKWAIER LEGKADAVET 

       550        560        570        580        590        600 
PIGFVPTGES IDLEGLDMTP AEVESAVRVD PAEWATELAS IEEWFANFGE SLPAALQSEL 


DGLKTRLG 

« Hide

References

[1]"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1."
Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J.
PLoS Genet. 2:2094-2106(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TC1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000474 Genomic DNA. Translation: ABM06961.1.
RefSeqYP_946629.1. NC_008711.1.

3D structure databases

ProteinModelPortalA1R317.
SMRA1R317. Positions 13-608.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290340.AAur_0832.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM06961; ABM06961; AAur_0832.
GeneID4641427.
KEGGaau:AAur_0832.
PATRIC20976990. VBIArtAur67810_0845.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAWMRFGED.
OrthoDBEOG6MPWQS.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycAAUR290340:GI59-832-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_ARTAT
AccessionPrimary (citable) accession number: A1R317
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways