Reviewed, UniProtKB/Swiss-Prot A1R1C5 (URE1_ARTAT)

Names and origin

Protein names

Recommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	AAur_0216

Organism

Arthrobacter aurescens (strain TC1) [Complete proteome] [HAMAP]

Taxonomic identifier

290340 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity.
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Urease subunit alpha HAMAP MF_01953		PRO_1000070646
Regions
	Domain	132 – 576	445	Urease
Sites
	Active site	323	1	Proton donor By similarity
	Metal binding	137	1	Nickel 2 By similarity
	Metal binding	139	1	Nickel 2 By similarity
	Metal binding	220	1	Nickel 1; via carbamate group By similarity
	Metal binding	220	1	Nickel 2; via carbamate group By similarity
	Metal binding	249	1	Nickel 1 By similarity
	Metal binding	275	1	Nickel 1 By similarity
	Metal binding	363	1	Nickel 2 By similarity
	Binding site	222	1	Substrate By similarity
Amino acid modifications
	Modified residue	220	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1R1C5-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 270C5C172C03AA67	FASTA	576	61,737
	10 20 30 40 50 60 MSFEIPRKQY AQLYGPTTGD SIRLADTELF LEIEKDYTVY GEEVVFGGGK VIRDGMGQNG 70 80 90 100 110 120 QLTRAEDIPD TVITNVIVLD YTGIYKADIA LKDGHIFKIG KAGNPQITDG VDIVIGASTE 130 140 150 160 170 180 IIAGERKILT AGGIDTHIHF ISPEQVPAAL CNGITTMVGG GTGPAEGTKA TTITPGAWHI 190 200 210 220 230 240 SRMLQAAEGL PVNIGLFGKG HASAVEPLAE QIRAGAVGLK VHEDWGSTTS SIDMSLRVAD 250 260 270 280 290 300 EYDVQIAIHT DTLNECGFVE DTIRAIDGRV IHTFHTEGAG GGHAPDIIKI AGLPNVLPAS 310 320 330 340 350 360 TNPTLPYTRN TIEEHLDMLM VCHHLNPDIP EDVAFADSRI RAETIAAEDV LHDLGIFAIT 370 380 390 400 410 420 SSDSQAMGRV GEVVTRTWQV ADAMKRQRGA LHDPSGAPHG SAESDNFRLK RYIAKYTINA 430 440 450 460 470 480 AIAQGMADVI GSVEEGKFAD LVLWDPAFFG VKPELVIKGG QIAYALMGDA NASIPTPQPR 490 500 510 520 530 540 TMRPMFATYG KALQQTSITF LSQAAIDAGV PAELGLQRII KPVSGIRNLT KADLKYNGET 550 560 570 PDIAVDPETY KVTVDGVEVT SQPSDVLPMA QRYFLF

References

[1]

"Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1." Mongodin E.F., Shapir N., Daugherty S.C., DeBoy R.T., Emerson J.B., Shvartzbeyn A., Radune D., Vamathevan J., Riggs F., Grinberg V., Khouri H.M., Wackett L.P., Nelson K.E., Sadowsky M.J. PLoS Genet. 2:2094-2106(2006) [PubMed: 17194220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000474 Genomic DNA. Translation: ABM07828.1.
RefSeq	YP_946037.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1R1C5.
Protein family/group databases
MEROPS	M38.982.
Genome annotation databases
GeneID	4637988.
GenomeReviews	Gene locus AAur_0216 in contig CP000474_GR.
KEGG	aau:AAur_0216.
TIGR	AAur_0216.
Phylogenomic databases
OMA	SHIHFIC.
Family and domain databases
HAMAP	MF_01953. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017952. Urease_asu_core. IPR017950. Urease_asu_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
TIGRFAMs	TIGR01792. urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_ARTAT

Accession

Primary (citable) accession number: A1R1C5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)