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A1R0Q9

- SYI_BORT9

UniProt

A1R0Q9 - SYI_BORT9

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BT0833
Organism
Borrelia turicatae (strain 91E135)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBTUR314724:GJ88-841-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BT0833
OrganismiBorrelia turicatae (strain 91E135)
Taxonomic identifieri314724 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
ProteomesiUP000001205: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Isoleucine--tRNA ligaseUniRule annotation
PRO_1000216252Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi314724.BT0833.

Structurei

3D structure databases

ProteinModelPortaliA1R0Q9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

A1R0Q9-1 [UniParc]FASTAAdd to Basket

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MFKKVESKVN FPKIEERILK FWNDNKIFEK SIQQREGCEE FTFYDGPPFA     50
TGLPHFGHFV PNTIKDIIPR YKTMKGKQVK RYFGWDTHGL PVEYEVEKSL 100
KISGRYEIEK YGIDKFNEEC RKIVLRYTKE WQKTISRLGR WIDFENNYKT 150
MDTTFMESVW WVFQTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
EIHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGK DIDYSKIFDK 250
EKDETFIIGT KRLNHYYKDE KAYTVIEQFK GEYIKGIEYE PIFNYFLNQR 300
DKGAFKIHTA EYVTTDDGTG IVHIAPFGEE DYNILKNNTK TDMITPIDAE 350
CRFTNEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENFLH RYPFCYRTNS 400
PLIYRPISSW FVNIEAIKEK LIKSNEQINW MPSHLKKGRF GKWLENARDW 450
AISRNRFWGN PIPVWICSKT GNKICIGSKE ELERLSGQKV NDLHKDKVDK 500
ITWPSEYGGV YVRTSEVLDC WFESGSMPYA SKHYPFKDKD NFHNIFPADF 550
IAEGLDQTRG WFYTLTILGT ALFENTAFKN VIVNGLVLSS DGRKMSKSLR 600
NYTDPMEVIN TFGADALRLY LVMSPVIRAD DLKYSDDGVK DVLKNIIIPI 650
WNAYSFFITY AIIDKFEPNN NINLHKTNIL DKWIVSEIES LKKILNEEID 700
KYNLTKSIEE LLAFIDKLNN WYIRRSRRRF WKSENDNDKI DAHETLYYVI 750
KNLMLMLAPF IPFLTEEIYQ NLKTKDEKES IHLNKYPQAI EKLINIDLEE 800
KMNFIRKVVS IARALRASHN IKIRKPISTI YVVTKDQKEQ QILNEMKEII 850
LEEINAKEIK IKSNEEELVT YKAKANFREL GSKLGVNMKV GSLEIMKLTN 900
EDILKIINGN KHIIKINENT YNITLKDIIL ERHERENLKI INEDSVTIGL 950
DALITEELYL EGLSRELIRK VQNLRKENNF NVSDRIILYI DNSDILKKIT 1000
NQFESYIKTE TLTLKIEINK EKALTNVELD DAIFIKIGIK RWSN 1044
Length:1,044
Mass (Da):122,959
Last modified:April 14, 2009 - v1
Checksum:i90243AEF3409511A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000049 Genomic DNA. Translation: AAX18150.1.
RefSeqiWP_011772768.1. NC_008710.1.
YP_945821.1. NC_008710.1.

Genome annotation databases

EnsemblBacteriaiAAX18150; AAX18150; BT0833.
GeneIDi4642651.
KEGGibtu:BT0833.
PATRICi20573411. VBIBorTur92789_0825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000049 Genomic DNA. Translation: AAX18150.1 .
RefSeqi WP_011772768.1. NC_008710.1.
YP_945821.1. NC_008710.1.

3D structure databases

ProteinModelPortali A1R0Q9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 314724.BT0833.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX18150 ; AAX18150 ; BT0833 .
GeneIDi 4642651.
KEGGi btu:BT0833.
PATRICi 20573411. VBIBorTur92789_0825.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BTUR314724:GJ88-841-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
    Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91E135.

Entry informationi

Entry nameiSYI_BORT9
AccessioniPrimary (citable) accession number: A1R0Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 14, 2009
Last modified: September 3, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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