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A1R0Q9

- SYI_BORT9

UniProt

A1R0Q9 - SYI_BORT9

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia turicatae (strain 91E135)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (14 Apr 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei597 – 5971ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBTUR314724:GJ88-841-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BT0833
    OrganismiBorrelia turicatae (strain 91E135)
    Taxonomic identifieri314724 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorrelia
    ProteomesiUP000001205: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10441044Isoleucine--tRNA ligasePRO_1000216252Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi314724.BT0833.

    Structurei

    3D structure databases

    ProteinModelPortaliA1R0Q9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A1R0Q9-1 [UniParc]FASTAAdd to Basket

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    MFKKVESKVN FPKIEERILK FWNDNKIFEK SIQQREGCEE FTFYDGPPFA     50
    TGLPHFGHFV PNTIKDIIPR YKTMKGKQVK RYFGWDTHGL PVEYEVEKSL 100
    KISGRYEIEK YGIDKFNEEC RKIVLRYTKE WQKTISRLGR WIDFENNYKT 150
    MDTTFMESVW WVFQTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
    EIHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGK DIDYSKIFDK 250
    EKDETFIIGT KRLNHYYKDE KAYTVIEQFK GEYIKGIEYE PIFNYFLNQR 300
    DKGAFKIHTA EYVTTDDGTG IVHIAPFGEE DYNILKNNTK TDMITPIDAE 350
    CRFTNEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENFLH RYPFCYRTNS 400
    PLIYRPISSW FVNIEAIKEK LIKSNEQINW MPSHLKKGRF GKWLENARDW 450
    AISRNRFWGN PIPVWICSKT GNKICIGSKE ELERLSGQKV NDLHKDKVDK 500
    ITWPSEYGGV YVRTSEVLDC WFESGSMPYA SKHYPFKDKD NFHNIFPADF 550
    IAEGLDQTRG WFYTLTILGT ALFENTAFKN VIVNGLVLSS DGRKMSKSLR 600
    NYTDPMEVIN TFGADALRLY LVMSPVIRAD DLKYSDDGVK DVLKNIIIPI 650
    WNAYSFFITY AIIDKFEPNN NINLHKTNIL DKWIVSEIES LKKILNEEID 700
    KYNLTKSIEE LLAFIDKLNN WYIRRSRRRF WKSENDNDKI DAHETLYYVI 750
    KNLMLMLAPF IPFLTEEIYQ NLKTKDEKES IHLNKYPQAI EKLINIDLEE 800
    KMNFIRKVVS IARALRASHN IKIRKPISTI YVVTKDQKEQ QILNEMKEII 850
    LEEINAKEIK IKSNEEELVT YKAKANFREL GSKLGVNMKV GSLEIMKLTN 900
    EDILKIINGN KHIIKINENT YNITLKDIIL ERHERENLKI INEDSVTIGL 950
    DALITEELYL EGLSRELIRK VQNLRKENNF NVSDRIILYI DNSDILKKIT 1000
    NQFESYIKTE TLTLKIEINK EKALTNVELD DAIFIKIGIK RWSN 1044
    Length:1,044
    Mass (Da):122,959
    Last modified:April 14, 2009 - v1
    Checksum:i90243AEF3409511A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000049 Genomic DNA. Translation: AAX18150.1.
    RefSeqiWP_011772768.1. NC_008710.1.
    YP_945821.1. NC_008710.1.

    Genome annotation databases

    EnsemblBacteriaiAAX18150; AAX18150; BT0833.
    GeneIDi4642651.
    KEGGibtu:BT0833.
    PATRICi20573411. VBIBorTur92789_0825.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000049 Genomic DNA. Translation: AAX18150.1 .
    RefSeqi WP_011772768.1. NC_008710.1.
    YP_945821.1. NC_008710.1.

    3D structure databases

    ProteinModelPortali A1R0Q9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 314724.BT0833.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX18150 ; AAX18150 ; BT0833 .
    GeneIDi 4642651.
    KEGGi btu:BT0833.
    PATRICi 20573411. VBIBorTur92789_0825.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BTUR314724:GJ88-841-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
      Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
      Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 91E135.

    Entry informationi

    Entry nameiSYI_BORT9
    AccessioniPrimary (citable) accession number: A1R0Q9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: April 14, 2009
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3