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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia turicatae (strain 91E135)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei597ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBTUR314724:G1G4D-850-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BT0833
OrganismiBorrelia turicatae (strain 91E135)
Taxonomic identifieri314724 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorrelia
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002162521 – 1044Isoleucine--tRNA ligaseAdd BLAST1044

Proteomic databases

PRIDEiA1R0Q9

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi314724.BT0833

Structurei

3D structure databases

ProteinModelPortaliA1R0Q9
SMRiA1R0Q9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 58"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

A1R0Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKKVESKVN FPKIEERILK FWNDNKIFEK SIQQREGCEE FTFYDGPPFA
60 70 80 90 100
TGLPHFGHFV PNTIKDIIPR YKTMKGKQVK RYFGWDTHGL PVEYEVEKSL
110 120 130 140 150
KISGRYEIEK YGIDKFNEEC RKIVLRYTKE WQKTISRLGR WIDFENNYKT
160 170 180 190 200
MDTTFMESVW WVFQTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK
210 220 230 240 250
EIHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGK DIDYSKIFDK
260 270 280 290 300
EKDETFIIGT KRLNHYYKDE KAYTVIEQFK GEYIKGIEYE PIFNYFLNQR
310 320 330 340 350
DKGAFKIHTA EYVTTDDGTG IVHIAPFGEE DYNILKNNTK TDMITPIDAE
360 370 380 390 400
CRFTNEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENFLH RYPFCYRTNS
410 420 430 440 450
PLIYRPISSW FVNIEAIKEK LIKSNEQINW MPSHLKKGRF GKWLENARDW
460 470 480 490 500
AISRNRFWGN PIPVWICSKT GNKICIGSKE ELERLSGQKV NDLHKDKVDK
510 520 530 540 550
ITWPSEYGGV YVRTSEVLDC WFESGSMPYA SKHYPFKDKD NFHNIFPADF
560 570 580 590 600
IAEGLDQTRG WFYTLTILGT ALFENTAFKN VIVNGLVLSS DGRKMSKSLR
610 620 630 640 650
NYTDPMEVIN TFGADALRLY LVMSPVIRAD DLKYSDDGVK DVLKNIIIPI
660 670 680 690 700
WNAYSFFITY AIIDKFEPNN NINLHKTNIL DKWIVSEIES LKKILNEEID
710 720 730 740 750
KYNLTKSIEE LLAFIDKLNN WYIRRSRRRF WKSENDNDKI DAHETLYYVI
760 770 780 790 800
KNLMLMLAPF IPFLTEEIYQ NLKTKDEKES IHLNKYPQAI EKLINIDLEE
810 820 830 840 850
KMNFIRKVVS IARALRASHN IKIRKPISTI YVVTKDQKEQ QILNEMKEII
860 870 880 890 900
LEEINAKEIK IKSNEEELVT YKAKANFREL GSKLGVNMKV GSLEIMKLTN
910 920 930 940 950
EDILKIINGN KHIIKINENT YNITLKDIIL ERHERENLKI INEDSVTIGL
960 970 980 990 1000
DALITEELYL EGLSRELIRK VQNLRKENNF NVSDRIILYI DNSDILKKIT
1010 1020 1030 1040
NQFESYIKTE TLTLKIEINK EKALTNVELD DAIFIKIGIK RWSN
Length:1,044
Mass (Da):122,959
Last modified:April 14, 2009 - v1
Checksum:i90243AEF3409511A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000049 Genomic DNA Translation: AAX18150.1
RefSeqiWP_011772768.1, NC_008710.1

Genome annotation databases

EnsemblBacteriaiAAX18150; AAX18150; BT0833
KEGGibtu:BT0833

Similar proteinsi

Entry informationi

Entry nameiSYI_BORT9
AccessioniPrimary (citable) accession number: A1R0Q9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 14, 2009
Last modified: April 25, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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