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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia turicatae (strain 91E135)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBTUR314724:GJ88-841-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BT0833
OrganismiBorrelia turicatae (strain 91E135)
Taxonomic identifieri314724 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorrelia
ProteomesiUP000001205 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Isoleucine--tRNA ligasePRO_1000216252Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi314724.BT0833.

Structurei

3D structure databases

ProteinModelPortaliA1R0Q9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

A1R0Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKKVESKVN FPKIEERILK FWNDNKIFEK SIQQREGCEE FTFYDGPPFA
60 70 80 90 100
TGLPHFGHFV PNTIKDIIPR YKTMKGKQVK RYFGWDTHGL PVEYEVEKSL
110 120 130 140 150
KISGRYEIEK YGIDKFNEEC RKIVLRYTKE WQKTISRLGR WIDFENNYKT
160 170 180 190 200
MDTTFMESVW WVFQTLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK
210 220 230 240 250
EIHDPSLTIK FKIKDKNEYL LAWTTTPWTL PTNLGIAVGK DIDYSKIFDK
260 270 280 290 300
EKDETFIIGT KRLNHYYKDE KAYTVIEQFK GEYIKGIEYE PIFNYFLNQR
310 320 330 340 350
DKGAFKIHTA EYVTTDDGTG IVHIAPFGEE DYNILKNNTK TDMITPIDAE
360 370 380 390 400
CRFTNEVKDF EGLFVKDADN KIIEKLKSMN LLFKRENFLH RYPFCYRTNS
410 420 430 440 450
PLIYRPISSW FVNIEAIKEK LIKSNEQINW MPSHLKKGRF GKWLENARDW
460 470 480 490 500
AISRNRFWGN PIPVWICSKT GNKICIGSKE ELERLSGQKV NDLHKDKVDK
510 520 530 540 550
ITWPSEYGGV YVRTSEVLDC WFESGSMPYA SKHYPFKDKD NFHNIFPADF
560 570 580 590 600
IAEGLDQTRG WFYTLTILGT ALFENTAFKN VIVNGLVLSS DGRKMSKSLR
610 620 630 640 650
NYTDPMEVIN TFGADALRLY LVMSPVIRAD DLKYSDDGVK DVLKNIIIPI
660 670 680 690 700
WNAYSFFITY AIIDKFEPNN NINLHKTNIL DKWIVSEIES LKKILNEEID
710 720 730 740 750
KYNLTKSIEE LLAFIDKLNN WYIRRSRRRF WKSENDNDKI DAHETLYYVI
760 770 780 790 800
KNLMLMLAPF IPFLTEEIYQ NLKTKDEKES IHLNKYPQAI EKLINIDLEE
810 820 830 840 850
KMNFIRKVVS IARALRASHN IKIRKPISTI YVVTKDQKEQ QILNEMKEII
860 870 880 890 900
LEEINAKEIK IKSNEEELVT YKAKANFREL GSKLGVNMKV GSLEIMKLTN
910 920 930 940 950
EDILKIINGN KHIIKINENT YNITLKDIIL ERHERENLKI INEDSVTIGL
960 970 980 990 1000
DALITEELYL EGLSRELIRK VQNLRKENNF NVSDRIILYI DNSDILKKIT
1010 1020 1030 1040
NQFESYIKTE TLTLKIEINK EKALTNVELD DAIFIKIGIK RWSN
Length:1,044
Mass (Da):122,959
Last modified:April 14, 2009 - v1
Checksum:i90243AEF3409511A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000049 Genomic DNA. Translation: AAX18150.1.
RefSeqiWP_011772768.1. NC_008710.1.
YP_945821.1. NC_008710.1.

Genome annotation databases

EnsemblBacteriaiAAX18150; AAX18150; BT0833.
KEGGibtu:BT0833.
PATRICi20573411. VBIBorTur92789_0825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000049 Genomic DNA. Translation: AAX18150.1.
RefSeqiWP_011772768.1. NC_008710.1.
YP_945821.1. NC_008710.1.

3D structure databases

ProteinModelPortaliA1R0Q9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi314724.BT0833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX18150; AAX18150; BT0833.
KEGGibtu:BT0833.
PATRICi20573411. VBIBorTur92789_0825.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBTUR314724:GJ88-841-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
    Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91E135.

Entry informationi

Entry nameiSYI_BORT9
AccessioniPrimary (citable) accession number: A1R0Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 14, 2009
Last modified: June 24, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.