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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Borrelia turicatae (strain 91E135)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91MagnesiumUniRule annotation
Metal bindingi69 – 691MagnesiumUniRule annotation
Metal bindingi144 – 1441MagnesiumUniRule annotation
Metal bindingi147 – 1471MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBTUR314724:GJ88-24-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:BT0023A
OrganismiBorrelia turicatae (strain 91E135)
Taxonomic identifieri314724 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorrelia
ProteomesiUP000001205 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Crossover junction endodeoxyribonuclease RuvCPRO_1000195238Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi314724.BT0023A.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012182.
KOiK01159.
OMAiVGYGIIE.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.

Sequencei

Sequence statusi: Complete.

A1QYH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIMRILGIDP GLANVGWGIL DRKGSKYMYV QDGTVITHPF MSLKDRLKLI
60 70 80 90 100
SDEIILIIDN FKPDVASIEN IYFAKNKKTA ISVSEARGAI ILTFALKNLD
110 120 130 140 150
FYEYTPIQVK NSISGFGRIE KVQVKYVIRI LLGMKPDFIF TSDHSSDALA
160
LAICHGNYHI L
Length:161
Mass (Da):18,110
Last modified:April 14, 2009 - v1
Checksum:i10280CA56AA667F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000049 Genomic DNA. Translation: AAX17369.1.

Genome annotation databases

EnsemblBacteriaiAAX17369; AAX17369; BT0023A.
KEGGibtu:BT0023A.
PATRICi20571741. VBIBorTur92789_0024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000049 Genomic DNA. Translation: AAX17369.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi314724.BT0023A.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX17369; AAX17369; BT0023A.
KEGGibtu:BT0023A.
PATRICi20571741. VBIBorTur92789_0024.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012182.
KOiK01159.
OMAiVGYGIIE.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciBTUR314724:GJ88-24-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Borrelia hermsii and Borrelia turicatae: comparative analysis of two agents of endemic N. America relapsing fever."
    Porcella S.F., Raffel S.J., Schrumpf M.E., Montgomery B., Smith T., Schwan T.G.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91E135.

Entry informationi

Entry nameiRUVC_BORT9
AccessioniPrimary (citable) accession number: A1QYH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 14, 2009
Last modified: July 22, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.