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Protein

Pleckstrin homology domain-containing family G member 3

Gene

PLEKHG3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family G member 3
Short name:
PH domain-containing family G member 3
Gene namesi
Name:PLEKHG3
Synonyms:KIAA0599
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20364. PLEKHG3.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134925358.

Polymorphism and mutation databases

BioMutaiPLEKHG3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12191219Pleckstrin homology domain-containing family G member 3PRO_0000306863Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei76 – 761PhosphoserineCombined sources
Modified residuei433 – 4331PhosphoserineBy similarity
Modified residuei576 – 5761PhosphoserineCombined sources
Modified residuei577 – 5771PhosphoserineCombined sources
Modified residuei618 – 6181PhosphoserineBy similarity
Modified residuei631 – 6311PhosphoserineCombined sources
Modified residuei640 – 6401PhosphoserineCombined sources
Modified residuei643 – 6431PhosphoserineCombined sources
Modified residuei647 – 6471PhosphoserineCombined sources
Modified residuei741 – 7411PhosphoserineCombined sources
Modified residuei779 – 7791PhosphoserineBy similarity
Modified residuei827 – 8271PhosphoserineCombined sources
Modified residuei962 – 9621PhosphoserineCombined sources
Modified residuei1011 – 10111PhosphoserineCombined sources
Modified residuei1023 – 10231PhosphoserineBy similarity
Modified residuei1037 – 10371PhosphoserineCombined sources
Modified residuei1040 – 10401PhosphoserineCombined sources
Modified residuei1081 – 10811PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA1L390.
MaxQBiA1L390.
PeptideAtlasiA1L390.
PRIDEiA1L390.

PTM databases

iPTMnetiA1L390.
PhosphoSiteiA1L390.

Expressioni

Gene expression databases

BgeeiA1L390.
CleanExiHS_PLEKHG3.
ExpressionAtlasiA1L390. baseline and differential.
GenevisibleiA1L390. HS.

Interactioni

Protein-protein interaction databases

BioGridi117496. 23 interactions.
IntActiA1L390. 21 interactions.

Structurei

3D structure databases

ProteinModelPortaliA1L390.
SMRiA1L390. Positions 97-385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 272180DHPROSITE-ProRule annotationAdd
BLAST
Domaini296 – 39499PHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 6764Ser-richAdd
BLAST
Compositional biasi578 – 5825Poly-Glu

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000118925.
HOVERGENiHBG071520.
InParanoidiA1L390.
OMAiNMVEPPL.
OrthoDBiEOG7K9K2H.
PhylomeDBiA1L390.
TreeFamiTF328565.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A1L390-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVSTSLHQD GSQERPVSLT STTSSSGSSC DSRSAMEEPS SSEAPAKNGA
60 70 80 90 100
GSLRSRHLPN SNNNSSSWLN VKGPLSPFNS RAAAGPAHHK LSYLGRVVRE
110 120 130 140 150
IVETERMYVQ DLRSIVEDYL LKIIDTPGLL KPEQVSALFG NIENIYALNS
160 170 180 190 200
QLLRDLDSCN SDPVAVASCF VERSQEFDIY TQYCNNYPNS VAALTECMRD
210 220 230 240 250
KQQAKFFRDR QELLQHSLPL GSYLLKPVQR ILKYHLLLQE IAKHFDEEED
260 270 280 290 300
GFEVVEDAID TMTCVAWYIN DMKRRHEHAV RLQEIQSLLI NWKGPDLTTY
310 320 330 340 350
GELVLEGTFR VHRVRNERTF FLFDKTLLIT KKRGDHFVYK GNIPCSSLML
360 370 380 390 400
IESTRDSLCF TVTHYKHSKQ QYSIQAKTVE EKRNWTHHIK RLILENHHAT
410 420 430 440 450
IPQKAKEAIL EMDSYYPNRY RCSPERLKKA WSSQDEVSTN VRQGRRQSEP
460 470 480 490 500
TKHLLRQLNE KARAAGMKGK GRRESESSRS SRRPSGRSPT STEKRMSFES
510 520 530 540 550
ISSLPEVEPD PEAGSEQEVF SAVEGPSAEE TPSDTESPEV LETQLDAHQG
560 570 580 590 600
LLGMDPPGDM VDFVAAESTE DLKALSSEEE EEMGGAAQEP ESLLPPSVLD
610 620 630 640 650
QASVIAERFV SSFSRRSSVA QEDSKSSGFG SPRLVSRSSS VLSLEGSEKG
660 670 680 690 700
LARHGSATDS LSCQLSPEVD ISVGVATEDS PSVNGMEPPS PGCPVEPDRS
710 720 730 740 750
SCKKKESALS TRDRLLLDKI KSYYENAEHH DAGFSVRRRE SLSYIPKGLV
760 770 780 790 800
RNSISRFNSL PRPDPEPVPP VGSKRQVGSR PTSWALFELP GPSQAVKGDP
810 820 830 840 850
PPISDAEFRP SSEIVKIWEG MESSGGSPGK GPGQGQANGF DLHEPLFILE
860 870 880 890 900
EHELGAITEE SATASPESSS PTEGRSPAHL ARELKELVKE LSSSTQGELV
910 920 930 940 950
APLHPRIVQL SHVMDSHVSE RVKNKVYQLA RQYSLRIKSN KPVMARPPLQ
960 970 980 990 1000
WEKVAPERDG KSPTVPCLQE EAGEPLGGKG KRKPVLSLFD YEQLMAQEHS
1010 1020 1030 1040 1050
PPKPSSAGEM SPQRFFFNPS AVSQRTTSPG GRPSARSPLS PTETFSWPDV
1060 1070 1080 1090 1100
RELCSKYASR DEARRAGGGR PRGPPVNRSH SVPENMVEPP LSGRVGRCRS
1110 1120 1130 1140 1150
LSTKRGRGGG EAAQSPGPLP QSKPDGGETL YVTADLTLED NRRVIVMEKG
1160 1170 1180 1190 1200
PLPSPTAGLE ESSGQGPSSP VALLGQVQDF QQSAECQPKE EGSRDPADPS
1210
QQGRVRNLRE KFQALNSVG
Length:1,219
Mass (Da):134,412
Last modified:February 6, 2007 - v1
Checksum:iDAEB5673340F4FBD
GO
Isoform 2 (identifier: A1L390-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-466: Missing.
     467-468: MK → M

Show »
Length:752
Mass (Da):80,886
Checksum:iED46C97F19A331BC
GO
Isoform 3 (identifier: A1L390-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-173: Missing.

Note: No experimental confirmation available.
Show »
Length:1,163
Mass (Da):128,247
Checksum:i110333017DAC3A6F
GO

Sequence cautioni

The sequence BAB84995.1 differs from that shown.Intron retention. There are two regions of intron retention within the sequence which cause it to shift frame.Curated
The sequence CAD66586.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti443 – 46826QGRRQ…AAGMK → KGAGPEPPGSEEEEEEQEES LAVAEQ in AAH04298 (PubMed:15489334).CuratedAdd
BLAST
Sequence conflicti461 – 4688KARAAGMK → ESLAVAEQ in AAH73907 (PubMed:15489334).Curated
Sequence conflicti723 – 73614YYENA…AGFSV → WWPHCTPASCSSPT in AAH04298 (PubMed:15489334).CuratedAdd
BLAST
Sequence conflicti921 – 9211R → S in AAI29953 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1014 – 10141R → H.
Corresponds to variant rs41309246 [ dbSNP | Ensembl ].
VAR_061518
Natural varianti1036 – 10361R → W.1 Publication
Corresponds to variant rs229649 [ dbSNP | Ensembl ].
VAR_035330

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 466466Missing in isoform 2. 1 PublicationVSP_028533Add
BLAST
Alternative sequencei118 – 17356Missing in isoform 3. 1 PublicationVSP_028534Add
BLAST
Alternative sequencei467 – 4682MK → M in isoform 2. 1 PublicationVSP_041513

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL121774 Genomic DNA. No translation available.
BC004298 mRNA. Translation: AAH04298.1.
BC011891 mRNA. Translation: AAH11891.2.
BC063554 mRNA. Translation: AAH63554.1.
BC073907 mRNA. Translation: AAH73907.1.
BC129952 mRNA. Translation: AAI29953.1.
BC129953 mRNA. Translation: AAI29954.1.
BX248779 mRNA. Translation: CAD66586.1. Different initiation.
AK074169 mRNA. Translation: BAB84995.1. Sequence problems.
AB011171 mRNA. Translation: BAA25525.1.
CCDSiCCDS76690.1. [A1L390-1]
PIRiT00267.
RefSeqiNP_001295076.1. NM_001308147.1. [A1L390-1]
UniGeneiHs.509637.

Genome annotation databases

EnsembliENST00000247226; ENSP00000247226; ENSG00000126822. [A1L390-1]
ENST00000394691; ENSP00000378183; ENSG00000126822. [A1L390-3]
ENST00000471182; ENSP00000450945; ENSG00000126822. [A1L390-2]
GeneIDi26030.
KEGGihsa:26030.
UCSCiuc001xhn.2. human. [A1L390-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL121774 Genomic DNA. No translation available.
BC004298 mRNA. Translation: AAH04298.1.
BC011891 mRNA. Translation: AAH11891.2.
BC063554 mRNA. Translation: AAH63554.1.
BC073907 mRNA. Translation: AAH73907.1.
BC129952 mRNA. Translation: AAI29953.1.
BC129953 mRNA. Translation: AAI29954.1.
BX248779 mRNA. Translation: CAD66586.1. Different initiation.
AK074169 mRNA. Translation: BAB84995.1. Sequence problems.
AB011171 mRNA. Translation: BAA25525.1.
CCDSiCCDS76690.1. [A1L390-1]
PIRiT00267.
RefSeqiNP_001295076.1. NM_001308147.1. [A1L390-1]
UniGeneiHs.509637.

3D structure databases

ProteinModelPortaliA1L390.
SMRiA1L390. Positions 97-385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117496. 23 interactions.
IntActiA1L390. 21 interactions.

PTM databases

iPTMnetiA1L390.
PhosphoSiteiA1L390.

Polymorphism and mutation databases

BioMutaiPLEKHG3.

Proteomic databases

EPDiA1L390.
MaxQBiA1L390.
PeptideAtlasiA1L390.
PRIDEiA1L390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000247226; ENSP00000247226; ENSG00000126822. [A1L390-1]
ENST00000394691; ENSP00000378183; ENSG00000126822. [A1L390-3]
ENST00000471182; ENSP00000450945; ENSG00000126822. [A1L390-2]
GeneIDi26030.
KEGGihsa:26030.
UCSCiuc001xhn.2. human. [A1L390-1]

Organism-specific databases

CTDi26030.
GeneCardsiPLEKHG3.
H-InvDBHIX0011738.
HGNCiHGNC:20364. PLEKHG3.
neXtProtiNX_A1L390.
PharmGKBiPA134925358.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00760000118925.
HOVERGENiHBG071520.
InParanoidiA1L390.
OMAiNMVEPPL.
OrthoDBiEOG7K9K2H.
PhylomeDBiA1L390.
TreeFamiTF328565.

Miscellaneous databases

GenomeRNAii26030.
PROiA1L390.

Gene expression databases

BgeeiA1L390.
CleanExiHS_PLEKHG3.
ExpressionAtlasiA1L390. baseline and differential.
GenevisibleiA1L390. HS.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT TRP-1036.
    Tissue: Brain, Lung, Pancreas and Urinary bladder.
  3. "Full-length cDNA libraries and normalization."
    Li W.B., Gruber C., Jessee J., Polayes D.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-659 (ISOFORM 3).
    Tissue: Cervix carcinoma.
  4. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 177-1219 (ISOFORM 1).
    Tissue: Spleen.
  5. "Characterization of cDNA clones in size-fractionated cDNA libraries from human brain."
    Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O.
    DNA Res. 4:345-349(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 523-1219.
    Tissue: Brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76; SER-576; SER-577; SER-643; SER-647; SER-741; SER-962; SER-1037 AND SER-1040, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76; SER-576; SER-577; SER-1037 AND SER-1040, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76; SER-576; SER-577; SER-631; SER-640; SER-643; SER-647; SER-741; SER-827; SER-1037 AND SER-1040, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76; SER-631; SER-640; SER-643; SER-647; SER-741; SER-827; SER-1037; SER-1040 AND SER-1081, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-741 AND SER-1011, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPKHG3_HUMAN
AccessioniPrimary (citable) accession number: A1L390
Secondary accession number(s): A1L389
, B5MEC9, O60339, Q6GMS3, Q6P4B1, Q7L3S3, Q86SW7, Q8TEF5, Q96EW6, Q9BT82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: February 6, 2007
Last modified: July 6, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.