ID   A1L2H2_XENLA            Unreviewed;       913 AA.
AC   A1L2H2;
DT   06-FEB-2007, integrated into UniProtKB/TrEMBL.
DT   06-FEB-2007, sequence version 1.
DT   27-MAR-2024, entry version 88.
DE   RecName: Full=hexokinase {ECO:0000256|ARBA:ARBA00012324};
DE            EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324};
GN   Name=hk2.L {ECO:0000313|RefSeq:NP_001090603.1,
GN   ECO:0000313|Xenbase:XB-GENE-5817417};
GN   Synonyms=hk2 {ECO:0000313|RefSeq:NP_001090603.1,
GN   ECO:0000313|Xenbase:XB-GENE-5817417}, LOC100036846
GN   {ECO:0000313|EMBL:AAI29527.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355 {ECO:0000313|EMBL:AAI29527.1};
RN   [1] {ECO:0000313|RefSeq:NP_001090603.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=12454917; DOI=10.1002/dvdy.10174;
RA   Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W.,
RA   Richardson P.;
RT   "Genetic and genomic tools for Xenopus research: The NIH Xenopus
RT   initiative.";
RL   Dev. Dyn. 225:384-391(2002).
RN   [2] {ECO:0000313|EMBL:AAI29527.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen {ECO:0000313|EMBL:AAI29527.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|RefSeq:NP_001090603.1}
RP   IDENTIFICATION.
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000435};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC         Evidence={ECO:0000256|ARBA:ARBA00000435};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000417};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC         Evidence={ECO:0000256|ARBA:ARBA00000417};
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 1/4.
CC       {ECO:0000256|ARBA:ARBA00004888}.
CC   -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC       {ECO:0000256|ARBA:ARBA00005028}.
CC   -!- SIMILARITY: Belongs to the hexokinase family.
CC       {ECO:0000256|ARBA:ARBA00009225}.
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DR   EMBL; BC129526; AAI29527.1; -; mRNA.
DR   RefSeq; NP_001090603.1; NM_001097134.1.
DR   DNASU; 100036846; -.
DR   GeneID; 100036846; -.
DR   KEGG; xla:100036846; -.
DR   AGR; Xenbase:XB-GENE-5817417; -.
DR   CTD; 100036846; -.
DR   Xenbase; XB-GENE-5817417; hk2.L.
DR   OrthoDB; 5305217at2759; -.
DR   UniPathway; UPA00109; UER00180.
DR   UniPathway; UPA00242; -.
DR   Proteomes; UP000186698; Chromosome 3L.
DR   Bgee; 100036846; Expressed in spleen and 19 other cell types or tissues.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR   GO; GO:0004396; F:hexokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0001678; P:intracellular glucose homeostasis; IEA:InterPro.
DR   CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2.
DR   Gene3D; 3.30.420.40; -; 2.
DR   Gene3D; 3.40.367.20; -; 2.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR001312; Hexokinase.
DR   InterPro; IPR019807; Hexokinase_BS.
DR   InterPro; IPR022673; Hexokinase_C.
DR   InterPro; IPR022672; Hexokinase_N.
DR   PANTHER; PTHR19443; HEXOKINASE; 1.
DR   PANTHER; PTHR19443:SF1; HEXOKINASE-3; 1.
DR   Pfam; PF00349; Hexokinase_1; 2.
DR   Pfam; PF03727; Hexokinase_2; 2.
DR   PRINTS; PR00475; HEXOKINASE.
DR   SUPFAM; SSF53067; Actin-like ATPase domain; 4.
DR   PROSITE; PS00378; HEXOKINASE_1; 2.
DR   PROSITE; PS51748; HEXOKINASE_2; 2.
PE   2: Evidence at transcript level;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW   Kinase {ECO:0000256|ARBA:ARBA00022777};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000186698};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          20..215
FT                   /note="Hexokinase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00349"
FT   DOMAIN          222..453
FT                   /note="Hexokinase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03727"
FT   DOMAIN          464..661
FT                   /note="Hexokinase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00349"
FT   DOMAIN          667..901
FT                   /note="Hexokinase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03727"
SQ   SEQUENCE   913 AA;  99813 MW;  3B7D4656B5996153 CRC64;
     MVSPKGDQTH NDQQARHEQI ESCLQPFHLS QAQLLSVRDL MLGDMERGLM KTPQKPSTLK
     MLPTYVRSTP DGTERGDFLV LDLGGAQFRI LHVELKDSAK VEMESQVYAI PDEVMHGTGQ
     QLFDHVAECL GQFLRQQKLH QKVLPLGFTF SFPCVQSQLD KSVLISWTKG FHCSEVEGKD
     VVHLFREAIR RQGGYDVDVI AIVNDTVGAM MSCGYQDHSC EVGFIVGTGT NLCYMEEMGN
     VESVEGDEGK MCINTEWGAL GDDGELSSVQ TEVDEQVDQN SLVPGKQCFD KLVSGMYMGE
     IVRLILVKLA NQGLLFDGTT SQSLLTKGCI ETKHVSAIED DHFGLAAARK MLSVLGLNPS
     EQDCVNVQHV CSAVSTRSAN LCAAGLAAVA TRLQLNNHNL KITIGVDGSV YKQHPKFSER
     LHSALQHLAP ECQIKFLISE DGSGKGTAIV TAVAKRLAAQ RHQIDDILAP FQLSQAKLEE
     VKSQMRAEID IGLCKETHAG ATVKMLPTYV RSTPDGTERG DFIALDLGGT NFRVLYVHIG
     RREDAGVQIV SKTYTLPQEI IQGTGGQLFD HIIDCISEFQ SENNLRGRRL PLGFTFSFPC
     KQTNLDQGIL ITWTKGFSAS GCVGKDVVTL LREAALRKKN NDIIVVALVN DTVGTMMSCG
     YNDPACEIGL IVGTGTNACY MEELKNVELL DGDEGQMCVN MEWGAFGDNN CLEDITTSFD
     HDVDTFSINP GKQRYEKMIS GMYLGEIVRQ ILIVLTRRGI LFGGKISERL LTRDLFPTRF
     LSLIESDTLG LVQVRSILTE LGLRSTCDDT MLVKEVCTTV SRRAAQLCAA GVAAVVEKMR
     ANRGLDQLKV TVGVDGTLYK LHPHFAGVVQ ETVKILAPKC DVTFLQSDDG SGRGAALITA
     VACRIAGAEA RAA
//