Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase

Gene

urad

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.1 Publication

Catalytic activityi

5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO2.

Pathwayi: urate degradation

This protein is involved in step 3 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (uox)
  2. 5-hydroxyisourate hydrolase (urah)
  3. 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (urad)
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67Proton donorBy similarity1
Binding sitei68Substrate; via carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

BRENDAi4.1.1.97. 928.
UniPathwayiUPA00394; UER00652.

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (EC:4.1.1.97)
Short name:
OHCU decarboxylase
Alternative name(s):
Parahox neighbor
Ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase
Gene namesi
Name:urad
Synonyms:prhoxnb
ORF Names:zgc:158663
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 24

Organism-specific databases

ZFINiZDB-GENE-070112-472. urad.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67H → N: Loss of activity. 1 Publication1
Mutagenesisi87E → Q: Loss of activity. 1 Publication1
Mutagenesisi161R → Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003152431 – 1742-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylaseAdd BLAST174

Proteomic databases

PaxDbiA1L259.
PeptideAtlasiA1L259.

Expressioni

Gene expression databases

BgeeiENSDARG00000071579.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi7955.ENSDARP00000096695.

Structurei

Secondary structure

1174
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 7Combined sources5
Helixi11 – 18Combined sources8
Helixi26 – 32Combined sources7
Helixi33 – 35Combined sources3
Helixi41 – 54Combined sources14
Helixi57 – 65Combined sources9
Helixi73 – 77Combined sources5
Helixi82 – 90Combined sources9
Turni91 – 94Combined sources4
Helixi98 – 115Combined sources18
Helixi123 – 125Combined sources3
Helixi128 – 138Combined sources11
Helixi143 – 165Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O70X-ray1.80A/B/C/D/E/F1-174[»]
2O73X-ray1.80A/B/C/D/E/F1-174[»]
2O74X-ray1.80A/B/C/D/E/F1-174[»]
ProteinModelPortaliA1L259.
SMRiA1L259.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA1L259.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 88Substrate binding5
Regioni119 – 123Substrate binding5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi172 – 174Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the OHCU decarboxylase family.Curated

Phylogenomic databases

eggNOGiENOG410IX4N. Eukaryota.
COG3195. LUCA.
GeneTreeiENSGT00390000002395.
HOGENOMiHOG000251764.
HOVERGENiHBG108651.
InParanoidiA1L259.
KOiK13485.
OMAiELQTCIN.
OrthoDBiEOG091G0RLD.
PhylomeDBiA1L259.
TreeFamiTF323276.

Family and domain databases

Gene3Di1.10.3330.10. 1 hit.
InterProiIPR018020. OHCU_decarboxylase.
IPR017580. OHCU_decarboxylase-1.
[Graphical view]
PfamiPF09349. OHCU_decarbox. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03164. UHCUDC. 1 hit.

Sequencei

Sequence statusi: Complete.

A1L259-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDINVVNALA YEDFVKLFGN VVEKCPLISA AIWSYRPFKD LADIEARISE
60 70 80 90 100
FIHSLPDSGK EGILRCHPDL AGRDLQSGTL TPESQEEQSQ AGMTTLDSAE
110 120 130 140 150
IVHMYRLNSE YKERFGFPFV ICARLNNKAD IVRQLSERLK NRRTAELECA
160 170
IEEVKKICSL RLHSIVLSDI QTKL
Length:174
Mass (Da):19,743
Last modified:February 6, 2007 - v1
Checksum:i1B408D09F29FF4D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF197726 mRNA. Translation: ABP04232.1.
BC129362 mRNA. Translation: AAI29363.1.
RefSeqiNP_001073641.1. NM_001080172.1.
UniGeneiDr.87169.

Genome annotation databases

EnsembliENSDART00000105917; ENSDARP00000096695; ENSDARG00000071579.
GeneIDi557735.
KEGGidre:557735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF197726 mRNA. Translation: ABP04232.1.
BC129362 mRNA. Translation: AAI29363.1.
RefSeqiNP_001073641.1. NM_001080172.1.
UniGeneiDr.87169.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O70X-ray1.80A/B/C/D/E/F1-174[»]
2O73X-ray1.80A/B/C/D/E/F1-174[»]
2O74X-ray1.80A/B/C/D/E/F1-174[»]
ProteinModelPortaliA1L259.
SMRiA1L259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000096695.

Proteomic databases

PaxDbiA1L259.
PeptideAtlasiA1L259.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000105917; ENSDARP00000096695; ENSDARG00000071579.
GeneIDi557735.
KEGGidre:557735.

Organism-specific databases

CTDi646625.
ZFINiZDB-GENE-070112-472. urad.

Phylogenomic databases

eggNOGiENOG410IX4N. Eukaryota.
COG3195. LUCA.
GeneTreeiENSGT00390000002395.
HOGENOMiHOG000251764.
HOVERGENiHBG108651.
InParanoidiA1L259.
KOiK13485.
OMAiELQTCIN.
OrthoDBiEOG091G0RLD.
PhylomeDBiA1L259.
TreeFamiTF323276.

Enzyme and pathway databases

UniPathwayiUPA00394; UER00652.
BRENDAi4.1.1.97. 928.

Miscellaneous databases

EvolutionaryTraceiA1L259.
PROiA1L259.

Gene expression databases

BgeeiENSDARG00000071579.

Family and domain databases

Gene3Di1.10.3330.10. 1 hit.
InterProiIPR018020. OHCU_decarboxylase.
IPR017580. OHCU_decarboxylase-1.
[Graphical view]
PfamiPF09349. OHCU_decarbox. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03164. UHCUDC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiURAD_DANRE
AccessioniPrimary (citable) accession number: A1L259
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.