Reviewed, UniProtKB/Swiss-Prot A1L251 (ENASE_DANRE)

Names and origin

Protein names

Recommended name:
    Cytosolic endo-beta-N-acetylglucosaminidase
      Short name=ENGase
    EC=3.2.1.96

Gene names

Name:	engase
ORF Names:	zgc:158649

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol By similarity.
Catalytic activity	Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 85 family. Contains 1 BRCT domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Cytosolic endo-beta-N-acetylglucosaminidase		PRO_0000328870
Regions
	Domain	270 – 362	93	BRCT

Sequences

	Sequence		Length	Mass (Da)
	A1L251-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C168C63E9C587DED	FASTA	713	80,255
	10 20 30 40 50 60 MIARKRKSNG SETTSGKIPK DDVSSESCLD QPADESVHEV VTFEPSTLPS VHYDPDTTEP 70 80 90 100 110 120 ISCSLKSLDE LLSWKRNEAS IFNVSSVPLA SRYPPLESCP RRTLVSHDMM GGYLEDRFIQ 130 140 150 160 170 180 GAEVETPYAF YHWEYIDIFN YFSHQMVTIP PAVWTNAAHR HGVLSIGTFI TEWTDGAKTC 190 200 210 220 230 240 EAFLADEESY RAAADKLVQI SHCNGFDGWL INIENELSET AVNNTGPFLR YLTDQMHERV 250 260 270 280 290 300 PGSVVIWYDS VLKDGKLLWQ NELNDNNRMF FDACDGFFTN YNWTEQSLEG MKSYAAAQGR 310 320 330 340 350 360 FADIYVGVDV FARGKVIGGK YETNKALELI RKYDLSTAIF APDWVYECHE KADFRQNQDK 370 380 390 400 410 420 FWSLLSDFLY IHRPSSNLPF VSSFCQGFGK SLYWRGKVET ERSWFNLHAQ EIQPLYLSES 430 440 450 460 470 480 FGNGGWLRTR GCSEDAWIGG SSLMLEGMIP SGLSDVCARI FSLHVPLAAR TFVSFVFKPP 490 500 510 520 530 540 VGVKVSLELK TIDGPLCTFD GTEEIASRSV FPEALAESNQ LVEQFAQNCG QWASDGWATR 550 560 570 580 590 600 CFLLKMIGCS LREVCIRVSR DGGDEDINFN CRIGEIMLLD ADNLQAPLQS VEGICVNDVV 610 620 630 640 650 660 WQTGVLKGDG HTLKVLLNAT LRWQYPTRQV RHFRIHWRHL RGPDPRIPSG PLTLIGRSYS 670 680 690 700 710 ALYRVVELEV PAAPGLIELV VEPVSKEGFS VPEAQWGRQT LSYSQSPSGN PSH

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC129351 mRNA. Translation: AAI29352.1.
IPI	IPI00829146.
RefSeq	NP_001074047.1.
UniGene	Dr.110643
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	A1L251.
Genome annotation databases
Ensembl	ENSDART00000002568; ENSDARP00000005952; ENSDARG00000010035; Danio rerio. [Genome view]
GeneID	561239.
KEGG	dre:561239.
Organism-specific databases
ZFIN	ZDB-GENE-070112-1332. zgc:158649.
Phylogenomic databases
HOVERGEN	A1L251.
Enzyme and pathway databases
BRENDA	3.2.1.96. 96826.
Gene expression databases
Bgee	A1L251.
Family and domain databases
InterPro	IPR005201. Glyco_hydro_85. [Graphical view]
PANTHER	PTHR13246. Glyco_hydro_85. 1 hit.
Pfam	PF03644. Glyco_hydro_85. 1 hit. [Graphical view]
PROSITE	PS50172. BRCT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENASE_DANRE

Accession

Primary (citable) accession number: A1L251

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	February 6, 2007
Last modified:	July 28, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project