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A1L251 (ENASE_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 6, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytosolic endo-beta-N-acetylglucosaminidase
Short name=ENGase
EC=3.2.1.96
Gene names
Name:engase
ORF Names:zgc:158649
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length713 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol By similarity.

Catalytic activity

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

Subcellular location

Cytoplasm › cytosol By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 85 family.

Contains 1 BRCT domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 713713Cytosolic endo-beta-N-acetylglucosaminidase
PRO_0000328870

Regions

Domain270 – 36293BRCT

Sequences

Sequence LengthMass (Da)Tools
A1L251 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: C168C63E9C587DED

FASTA71380,255
        10         20         30         40         50         60 
MIARKRKSNG SETTSGKIPK DDVSSESCLD QPADESVHEV VTFEPSTLPS VHYDPDTTEP 

        70         80         90        100        110        120 
ISCSLKSLDE LLSWKRNEAS IFNVSSVPLA SRYPPLESCP RRTLVSHDMM GGYLEDRFIQ 

       130        140        150        160        170        180 
GAEVETPYAF YHWEYIDIFN YFSHQMVTIP PAVWTNAAHR HGVLSIGTFI TEWTDGAKTC 

       190        200        210        220        230        240 
EAFLADEESY RAAADKLVQI SHCNGFDGWL INIENELSET AVNNTGPFLR YLTDQMHERV 

       250        260        270        280        290        300 
PGSVVIWYDS VLKDGKLLWQ NELNDNNRMF FDACDGFFTN YNWTEQSLEG MKSYAAAQGR 

       310        320        330        340        350        360 
FADIYVGVDV FARGKVIGGK YETNKALELI RKYDLSTAIF APDWVYECHE KADFRQNQDK 

       370        380        390        400        410        420 
FWSLLSDFLY IHRPSSNLPF VSSFCQGFGK SLYWRGKVET ERSWFNLHAQ EIQPLYLSES 

       430        440        450        460        470        480 
FGNGGWLRTR GCSEDAWIGG SSLMLEGMIP SGLSDVCARI FSLHVPLAAR TFVSFVFKPP 

       490        500        510        520        530        540 
VGVKVSLELK TIDGPLCTFD GTEEIASRSV FPEALAESNQ LVEQFAQNCG QWASDGWATR 

       550        560        570        580        590        600 
CFLLKMIGCS LREVCIRVSR DGGDEDINFN CRIGEIMLLD ADNLQAPLQS VEGICVNDVV 

       610        620        630        640        650        660 
WQTGVLKGDG HTLKVLLNAT LRWQYPTRQV RHFRIHWRHL RGPDPRIPSG PLTLIGRSYS 

       670        680        690        700        710 
ALYRVVELEV PAAPGLIELV VEPVSKEGFS VPEAQWGRQT LSYSQSPSGN PSH

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Olfactory epithelium.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC129351 mRNA. Translation: AAI29352.1.
IPIIPI00829146.
RefSeqNP_001074047.2. NM_001080578.2.
UniGeneDr.110643.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH85. Glycoside Hydrolase Family 85.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID561239.
KEGGdre:561239.

Organism-specific databases

ZFINZDB-GENE-070112-1332. zgc:158649.

Phylogenomic databases

eggNOGCOG4724.
HOGENOMHOG000082678.
HOVERGENHBG107848.
InParanoidA1L251.
KOK01227.
OrthoDBEOG44J2HF.

Family and domain databases

InterProIPR005201. Glyco_hydro_85.
[Graphical view]
PfamPF03644. Glyco_hydro_85. 1 hit.
[Graphical view]
PROSITEPS50172. BRCT. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio20883830.

Entry information

Entry nameENASE_DANRE
AccessionPrimary (citable) accession number: A1L251
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: February 6, 2007
Last modified: February 6, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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