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Protein

Cyclic AMP-responsive element-binding protein 3-like protein 2

Gene

creb3l2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/mbtps1 and S2P/mbtps2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis. May protect neuroblastoma cells from ER stress-induced death. In vitro activates transcription of target genes via direct binding to the CRE site.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation, Unfolded protein response

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-responsive element-binding protein 3-like protein 2
Short name:
cAMP-responsive element-binding protein 3-like protein 2
Cleaved into the following chain:
Gene namesi
Name:creb3l2
ORF Names:zgc:158603
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 4

Organism-specific databases

ZFINiZDB-GENE-070112-1542. creb3l2.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein By similarity

  • Note: ER membrane resident protein. Upon ER stress, translocated to the Golgi apparatus where it is cleaved. The cytosolic N-terminal fragment (processed cyclic AMP-responsive element-binding protein 3-like protein 1) is transported into the nucleus.By similarity
Processed cyclic AMP-responsive element-binding protein 3-like protein 2 :
  • Nucleus By similarity

  • Note: Upon ER stress, translocated into the nucleus.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 374374CytoplasmicSequence analysisAdd
BLAST
Transmembranei375 – 39521Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini396 – 519124LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Cyclic AMP-responsive element-binding protein 3-like protein 2PRO_0000288071Add
BLAST
Chaini1 – ?Processed cyclic AMP-responsive element-binding protein 3-like protein 2PRO_0000296213

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi485 – 4851N-linked (GlcNAc...)Sequence analysis
Glycosylationi503 – 5031N-linked (GlcNAc...)Sequence analysis
Glycosylationi516 – 5161N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/mbtps1 and S2P/mbtps2).By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiA1L224.

Expressioni

Gene expression databases

BgeeiA1L224.
ExpressionAtlasiA1L224. differential.

Interactioni

Subunit structurei

Binds DNA as a dimer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000087416.

Structurei

3D structure databases

ProteinModelPortaliA1L224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini291 – 35464bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni293 – 32230Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni333 – 35422Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi423 – 4264S1P recognitionBy similarity

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0709. Eukaryota.
ENOG410ZZQM. LUCA.
GeneTreeiENSGT00520000055538.
HOGENOMiHOG000060150.
HOVERGENiHBG057480.
InParanoidiA1L224.
KOiK09048.
OMAiMEDTHAV.
PhylomeDBiA1L224.
TreeFamiTF316079.

Family and domain databases

InterProiIPR029804. BBF2H7.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR22952:SF96. PTHR22952:SF96. 2 hits.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1L224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIMDSGEPF LQWDKNLSEL SEAGENDILY STHFTDLLDD LSQEALLGQL
60 70 80 90 100
LSDPFLSGRG DAMDTEEELT RASPVPPHIQ AEHSYSLCGD SRPQSPLSHL
110 120 130 140 150
PGEPGSDAAD SESDEWPMEQ EDKGIKMEPL LCVPLPALTL TVTPAGSAPE
160 170 180 190 200
PVIDSCDSAQ SLSLPQVKED SNSPQIKLEP HEVDQFLNLS PKGLECLQMP
210 220 230 240 250
PTPPSSVGSD SEGSQSPVHP CAPASPTQTP AVLKVAPRAP SSLSSSPLLT
260 270 280 290 300
APHKLQGSGP LLLTEEEKRT LIAEGYPVPT KLPLSKAEEK ALKKIRRKIK
310 320 330 340 350
NKISAQESRR KKKEYVDALE KKVETCSNEN HELRRKVENL ECTNKSLLQQ
360 370 380 390 400
LHSLQAVVAG KVPRSCRVTG TQTSTCLMVV VLCFSLFLGS FYPGLSPCSS
410 420 430 440 450
ITKADLSREI SIHDSYTTTV KSRSLLSIQE PGGLDEPHPI GLGGEYPEWD
460 470 480 490 500
RQADVMAAWR FEQQHKEEEA ELHKAEHRPL LLSTNETHAQ KAILIDLHTH
510
RSNETAKVIQ LDRTVNETS
Length:519
Mass (Da):56,901
Last modified:February 6, 2007 - v1
Checksum:iF1A6F0E891ADD496
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC129318 mRNA. Translation: AAI29319.1.
RefSeqiNP_001074120.1. NM_001080651.2.
UniGeneiDr.80606.

Genome annotation databases

EnsembliENSDART00000092984; ENSDARP00000087416; ENSDARG00000063563.
GeneIDi791169.
KEGGidre:791169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC129318 mRNA. Translation: AAI29319.1.
RefSeqiNP_001074120.1. NM_001080651.2.
UniGeneiDr.80606.

3D structure databases

ProteinModelPortaliA1L224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000087416.

Proteomic databases

PaxDbiA1L224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000092984; ENSDARP00000087416; ENSDARG00000063563.
GeneIDi791169.
KEGGidre:791169.

Organism-specific databases

CTDi64764.
ZFINiZDB-GENE-070112-1542. creb3l2.

Phylogenomic databases

eggNOGiKOG0709. Eukaryota.
ENOG410ZZQM. LUCA.
GeneTreeiENSGT00520000055538.
HOGENOMiHOG000060150.
HOVERGENiHBG057480.
InParanoidiA1L224.
KOiK09048.
OMAiMEDTHAV.
PhylomeDBiA1L224.
TreeFamiTF316079.

Miscellaneous databases

NextBioi20930573.
PROiA1L224.

Gene expression databases

BgeeiA1L224.
ExpressionAtlasiA1L224. differential.

Family and domain databases

InterProiIPR029804. BBF2H7.
IPR004827. bZIP.
[Graphical view]
PANTHERiPTHR22952:SF96. PTHR22952:SF96. 2 hits.
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: AB.

Entry informationi

Entry nameiCR3L2_DANRE
AccessioniPrimary (citable) accession number: A1L224
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: April 13, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.