A1L1K7 (ACBG2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Long-chain-fatty-acid--CoA ligase ACSBG2
EC=6.2.1.3

Alternative name(s):
Acyl-CoA synthetase bubblegum family member 2

Gene names

Name:

Acsbg2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	667 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Has increased ability to activate oleic and linoleic acid. May play a role in spermatogenesis By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.

Ontologies

Keywords
Biological process	Differentiation Fatty acid metabolism Lipid metabolism Spermatogenesis
Cellular component	Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain fatty acid-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 667	667	Long-chain-fatty-acid--CoA ligase ACSBG2		PRO_0000315814
Regions
	Nucleotide binding	227 – 235	9	ATP By similarity
	Nucleotide binding	418 – 423	6	ATP By similarity
Sites
	Binding site	496	1	ATP By similarity
	Binding site	511	1	ATP By similarity
	Binding site	624	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1L1K7 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: CE929821D95D6700	FASTA	667	74,266
	10 20 30 40 50 60 MTQEKKAEDP DRGMDTTSAA PRLWSTHCDG EVLLRLSKHG PGHETPMTIP ELFQESVERF 70 80 90 100 110 120 GAYPALASKN GKKWDTLTFS QYYDVCRKAA RSLIKLGLQR FHGVGILGFN SVEWVVAALG 130 140 150 160 170 180 AILAGGLCVG IYATNSAEAC QYVIKQANVN VLIVENDQQL QKILSIPPDK METVKAIVQY 190 200 210 220 230 240 RLPLMENSTN LYSWQDFMEL GNAIPNIQLD RVILSQKANQ CAVIIYTSGT TGSPKGVMLS 250 260 270 280 290 300 HDNITWTAGA MAREIELIHV SGKQDTIVSY LPLSHIAAQL MDIWIPIKVG VLTFFAQPDA 310 320 330 340 350 360 LRGTLVYTLQ EVKPTYFLGV PRVWEKMQDT IKENVAKSSN LRKKAFAWAK MLGLKVNTKK 370 380 390 400 410 420 MLGKRDIPMN YRMAKALVFT KVRTSLGLDN CHTFFSGASP LSQDVSEFFL SLDIPIGEIY 430 440 450 460 470 480 GMTECSGPHT VSCKSIYRVL SCGKVLNGCK NMLYKQNKDG VGEVCMWGRH VFMGYLGKED 490 500 510 520 530 540 ATLEVLDEDG WLHSGDIGRL DSHDFLYITG RIKEVLITAG GENIWPIPIE TLVKEKIPII 550 560 570 580 590 600 SHAMLVGDKA KFLSMLLTLK CETDQMSGEP LDKLNLEAIS FCQMLGSQAV TVSDILKIRD 610 620 630 640 650 660 PVVYTAIQYG IDIVNQQAVS DSHRIRKWII LEKDFSIQGG ELGPTSKLKR DLITQKYKAQ IDNMYSS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC129110 mRNA. Translation: AAI29111.1.
IPI	IPI00829486.
RefSeq	NP_001073565.1. NM_001080096.1.
UniGene	Rn.109833.
3D structure databases
ProteinModelPortal	A1L1K7.
ModBase	Search...
Proteomic databases
PRIDE	A1L1K7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	301120.
KEGG	rno:301120.
Organism-specific databases
CTD	81616.
RGD	1588580. Acsbg2.
Phylogenomic databases
HOVERGEN	HBG054660.
Gene expression databases
Genevestigator	A1L1K7.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K15013.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	648177.

Entry information

Entry name

ACBG2_RAT

Accession

Primary (citable) accession number: A1L1K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 16, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program