A1L1C2 (PLD6_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial cardiolipin hydrolase
EC=3.1.4.-

Alternative name(s):
Choline phosphatase 6
Mitochondrial phospholipase
Short name=MitoPLD
Phosphatidylcholine-hydrolyzing phospholipase D6
Phospholipase D6
Short name=PLD 6

Gene names

Name:

pld6

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cardiolipin hydrolase present at the mitochondrial outer membrane required both for mitochondrial fusion and piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting mitochondrial fusion. The production of phosphatidate also regulates the piRNA metabolic process by promoting recruitment and/or activation of components of the meiotic nuage, also named P granule, a critical step for primary biogenesis of piRNAs. Required during spermatogenesis to repress transposable elements and prevent their mobilization via its role in the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Domain	In contrast to other members of the phospholipase D family, contains only one PLD phosphodiesterase domain, suggesting that it has a single half-catalytic and requires homodimerization to form a complete active site By similarity.
Sequence similarities	Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily. Contains 1 PLD phosphodiesterase domain.

Ontologies

Keywords
Biological process	Differentiation Lipid degradation Meiosis Spermatogenesis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	DNA methylation involved in gamete generation Inferred from sequence or structural similarity. Source: UniProtKB P granule organization Inferred from sequence or structural similarity. Source: UniProtKB lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from sequence or structural similarity. Source: UniProtKB mitochondrial fusion Inferred from sequence or structural similarity. Source: UniProtKB piRNA metabolic process Inferred from sequence or structural similarity. Source: UniProtKB spermatid development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	cardiolipin hydrolase activity Inferred from sequence or structural similarity. Source: UniProtKB protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Mitochondrial cardiolipin hydrolase		PRO_0000325913
Regions
	Topological domain	1 – 5	5	Mitochondrial intermembrane Potential
	Transmembrane	6 – 24	19	Helical; Potential
	Topological domain	25 – 210	186	Cytoplasmic Potential
	Domain	138 – 165	28	PLD phosphodiesterase
Sites
	Active site	143	1	Potential
	Active site	145	1	Potential
	Active site	150	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A1L1C2 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 3218D8C5018A1ACD	FASTA	210	23,919
	10 20 30 40 50 60 MLLWGRWKLA AGLAGLALSL ELFYRYMRRR KPLREVLFFP VPVTCIEPVL SPMKQCSCPL 70 80 90 100 110 120 PHTDSAFSRL LVQLLGAQRS LELCVFTFSS PSLARALLIL HRRDVRVRVI TDNDYMAAPG 130 140 150 160 170 180 SQIGPLRSAG VAVRHDQSSG YMHHKFAVVD GTVVLTGSLN WTVQAFQSNK ENILITDDTV 190 200 210 IVKAYQKEFE RLWEEYDPAT YNFFPEKENK

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC128979 mRNA. Translation: AAI28980.1.
RefSeq	NP_001091258.1. NM_001097789.1.
UniGene	Xl.16857.
3D structure databases
ProteinModelPortal	A1L1C2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100037063.
KEGG	xla:100037063.
Organism-specific databases
CTD	201164.
Xenbase	XB-GENE-5729953. pld6.
Phylogenomic databases
HOVERGEN	HBG108267.
Family and domain databases
InterPro	IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
SMART	SM00155. PLDc. 1 hit. [Graphical view]
PROSITE	PS50035. PLD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLD6_XENLA

Accession

Primary (citable) accession number: A1L1C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 6, 2007
Last modified:	September 21, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program