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A1KXE4 (F168B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myelin-associated neurite-outgrowth inhibitor

Short name=Mani
Alternative name(s):
p20
Gene names
Name:FAM168B
Synonyms:KIAA0280L, MANI
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length195 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Modulates neuronal axonal outgrowth by acting as a negative regulator of CDC42 and STAT3 and a positive regulator of STMN2. Positive regulator of CDC27 By similarity.

Subunit structure

May form homodimers. May interact with DAZAP2, FAM168A, PRDX6, RBM6, TMTC1 and YPEL2. Interacts with CDC27. Ref.1 Ref.5

Subcellular location

Cytoplasmperinuclear region By similarity. Cell membrane; Multi-pass membrane protein By similarity. Cell projectionaxon By similarity. Note: Expressed in neuronal cell bodies and axonal fibers By similarity.

Tissue specificity

Expressed in the brain, within neuronal axonal fibers and associated with myelin sheets (at protein level). Expression tends to be lower in the brain of Alzheimer disease patients compared to healthy individuals (at protein level). Ref.1

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the FAM168 family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A1KXE4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A1KXE4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     78-195: NPYQTAVYPV...PTYSYVPPQW → QAVPMSPCAD...EALESLMDCN

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 195195Myelin-associated neurite-outgrowth inhibitor
PRO_0000325978

Regions

Topological domain1 – 1818Cytoplasmic Potential
Transmembrane19 – 4224Helical; Potential
Topological domain43 – 142100Extracellular Potential
Transmembrane143 – 16422Helical; Potential
Topological domain165 – 19531Cytoplasmic Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.4

Natural variations

Alternative sequence78 – 195118NPYQT…VPPQW → QAVPMSPCADQWARQLGQIS GQPFVLLAGLLCWPHGEALE SLMDCN in isoform 2.
VSP_032508

Experimental info

Sequence conflict71P → L in AAI10643. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: 27A0D0D74AB44A10

FASTA19520,324
        10         20         30         40         50         60 
MNPVYSPGSS GVPYANAKGI GYPAGFPMGY AAAAPAYSPN MYPGANPTFQ TGYTPGTPYK 

        70         80         90        100        110        120 
VSCSPTSGAV PPYSSSPNPY QTAVYPVRSA YPQQSPYAQQ GTYYTQPLYA APPHVIHHTT 

       130        140        150        160        170        180 
VVQPNGMPAT VYPAPIPPPR GNGVTMGMVA GTTMAMSAGT LLTAHSPTPV APHPVTVPTY 

       190 
RAPGTPTYSY VPPQW 

« Hide

Isoform 2 [UniParc].

Checksum: 3602279D84F60ECE
Show »

FASTA12312,707

References

« Hide 'large scale' references
[1]"The novel protein MANI modulates neurogenesis and neurite-cone growth."
Mishra M., Akatsu H., Heese K.
J. Cell. Mol. Med. 15:1713-1725(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), HOMODIMERIZATION, INTERACTION WITH CDC27; PRDX6 AND RBM6, TISSUE SPECIFICITY.
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Eye.
[4]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Characterizing the neurite outgrowth inhibitory effect of Mani."
Mishra M., Lee S., Lin M.K., Yamashita T., Heese K.
FEBS Lett. 586:3018-3023(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DAZAP2; FAM168A; TMTC1 AND YPEL2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY253283 mRNA. Translation: AAP79599.1.
CH471250 Genomic DNA. Translation: EAW51267.1.
BC066347 mRNA. Translation: AAH66347.1.
BC110642 mRNA. Translation: AAI10643.1.
CCDSCCDS42755.1. [A1KXE4-1]
RefSeqNP_001009993.2. NM_001009993.2. [A1KXE4-1]
XP_005263648.1. XM_005263591.2. [A1KXE4-1]
XP_006712326.1. XM_006712263.1. [A1KXE4-1]
XP_006712327.1. XM_006712264.1. [A1KXE4-1]
XP_006712328.1. XM_006712265.1. [A1KXE4-1]
XP_006712329.1. XM_006712266.1. [A1KXE4-1]
UniGeneHs.534679.

3D structure databases

ProteinModelPortalA1KXE4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126221. 1 interaction.
IntActA1KXE4. 2 interactions.
MINTMINT-6768637.
STRING9606.ENSP00000374565.

PTM databases

PhosphoSiteA1KXE4.

Proteomic databases

MaxQBA1KXE4.
PaxDbA1KXE4.
PRIDEA1KXE4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000389915; ENSP00000374565; ENSG00000152102. [A1KXE4-1]
ENST00000409185; ENSP00000387051; ENSG00000152102. [A1KXE4-1]
GeneID130074.
KEGGhsa:130074.
UCSCuc002tsd.3. human. [A1KXE4-1]

Organism-specific databases

CTD130074.
GeneCardsGC02M131805.
HGNCHGNC:27016. FAM168B.
HPAHPA043778.
neXtProtNX_A1KXE4.
PharmGKBPA162387098.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG28430.
HOGENOMHOG000073527.
HOVERGENHBG052179.
InParanoidA1KXE4.
OMAPFTNTKG.
OrthoDBEOG7HB5BT.
PhylomeDBA1KXE4.
TreeFamTF331128.

Gene expression databases

BgeeA1KXE4.
CleanExHS_FAM168B.
GenevestigatorA1KXE4.

Family and domain databases

InterProIPR029247. FAM168A/MANI.
[Graphical view]
PfamPF14944. TCRP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFAM168B. human.
GenomeRNAi130074.
NextBio82696.
PROA1KXE4.

Entry information

Entry nameF168B_HUMAN
AccessionPrimary (citable) accession number: A1KXE4
Secondary accession number(s): Q2TAZ6, Q6NZ40
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: February 20, 2007
Last modified: July 9, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM