A1KXE4 (F168B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myelin-associated neurite-outgrowth inhibitor
Short name=Mani

Alternative name(s):
p20

Gene names

Name:	FAM168B
Synonyms:	KIAA0280L, MANI

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Modulates neuronal axonal outgrowth by acting as a negative regulator of CDC42 and STAT3 and a positive regulator of STMN2. Positive regulator of CDC27 By similarity.
Subunit structure	May form homodimers. May interact with DAZAP2, FAM168A, PRDX6, RBM6, TMTC1 and YPEL2. Interacts with CDC27. Ref.1 Ref.4
Subcellular location	Cytoplasm › perinuclear region By similarity. Cell membrane; Multi-pass membrane protein By similarity. Cell projection › axon By similarity. Note: Expressed in neuronal cell bodies and axonal fibers By similarity.
Tissue specificity	Expressed in the brain, within neuronal axonal fibers and associated with myelin sheets (at protein level). Expression tends to be lower in the brain of Alzheimer disease patients compared to healthy individuals (at protein level). Ref.1
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the FAM168 protein family.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	axon Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A1KXE4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A1KXE4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     78-195: NPYQTAVYPV...PTYSYVPPQW → QAVPMSPCAD...EALESLMDCN

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Myelin-associated neurite-outgrowth inhibitor		PRO_0000325978
Regions
	Topological domain	1 – 18	18	Cytoplasmic Potential
	Transmembrane	19 – 42	24	Helical; Potential
	Topological domain	43 – 142	100	Extracellular Potential
	Transmembrane	143 – 164	22	Helical; Potential
	Topological domain	165 – 195	31	Cytoplasmic Potential
Natural variations
	Alternative sequence	78 – 195	118	NPYQT…VPPQW → QAVPMSPCADQWARQLGQIS GQPFVLLAGLLCWPHGEALE SLMDCN in isoform 2.		VSP_032508
Experimental info
	Sequence conflict	7	1	P → L in AAI10643. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 27A0D0D74AB44A10	FASTA	195	20,324
	10 20 30 40 50 60 MNPVYSPGSS GVPYANAKGI GYPAGFPMGY AAAAPAYSPN MYPGANPTFQ TGYTPGTPYK 70 80 90 100 110 120 VSCSPTSGAV PPYSSSPNPY QTAVYPVRSA YPQQSPYAQQ GTYYTQPLYA APPHVIHHTT 130 140 150 160 170 180 VVQPNGMPAT VYPAPIPPPR GNGVTMGMVA GTTMAMSAGT LLTAHSPTPV APHPVTVPTY 190 RAPGTPTYSY VPPQW
	Isoform 2 [UniParc]. Checksum: 3602279D84F60ECE	FASTA	123	12,707
	10 20 30 40 50 60 MNPVYSPGSS GVPYANAKGI GYPAGFPMGY AAAAPAYSPN MYPGANPTFQ TGYTPGTPYK 70 80 90 100 110 120 VSCSPTSGAV PPYSSSPQAV PMSPCADQWA RQLGQISGQP FVLLAGLLCW PHGEALESLM DCN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The novel protein MANI modulates neurogenesis and neurite-cone growth." Mishra M., Akatsu H., Heese K. J. Cell. Mol. Med. 15:1713-1725(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), HOMODIMERIZATION, INTERACTION WITH CDC27; PRDX6 AND RBM6, TISSUE SPECIFICITY.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Eye.
[4]	"Characterizing the neurite outgrowth inhibitory effect of Mani." Mishra M., Lee S., Lin M.K., Yamashita T., Heese K. FEBS Lett. 586:3018-3023(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DAZAP2; FAM168A; TMTC1 AND YPEL2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY253283 mRNA. Translation: AAP79599.1. CH471250 Genomic DNA. Translation: EAW51267.1. BC066347 mRNA. Translation: AAH66347.1. BC110642 mRNA. Translation: AAI10643.1.
IPI	IPI00434875. IPI00556592.
RefSeq	NP_001009993.2. NM_001009993.2.
UniGene	Hs.534679.
3D structure databases
ProteinModelPortal	A1KXE4.
ModBase	Search...
Protein-protein interaction databases
IntAct	A1KXE4. 1 interaction.
MINT	MINT-6768637.
STRING	9606.ENSP00000374565.
PTM databases
PhosphoSite	A1KXE4.
Proteomic databases
PaxDb	A1KXE4.
PRIDE	A1KXE4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000389915; ENSP00000374565; ENSG00000152102. ENST00000409185; ENSP00000387051; ENSG00000152102.
GeneID	130074.
KEGG	hsa:130074.
UCSC	uc002tsd.3. human.
Organism-specific databases
CTD	130074.
GeneCards	GC02M131805.
HGNC	HGNC:27016. FAM168B.
HPA	HPA043778.
neXtProt	NX_A1KXE4.
PharmGKB	PA162387098.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG28430.
HOGENOM	HOG000073527.
HOVERGEN	HBG052179.
InParanoid	A1KXE4.
OMA	PFTNTKG.
OrthoDB	EOG43FGZ1.
Gene expression databases
Bgee	A1KXE4.
CleanEx	HS_FAM168B.
Genevestigator	A1KXE4.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	FAM168B. human.
GenomeRNAi	130074.
NextBio	82696.

Entry information

Entry name

F168B_HUMAN

Accession

Primary (citable) accession number: A1KXE4
Secondary accession number(s): Q2TAZ6, Q6NZ40

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 20, 2007
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.