A1KW60 (LUXS_NEIMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase
EC=4.4.1.21

Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS

Gene names

Name:	luxS
Ordered Locus Names:	NMC1956

Organism

Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) [Complete proteome] [HAMAP]

Taxonomic identifier

272831 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria meningitidis › Neisseria meningitidis serogroup C

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP-Rule MF_00091
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP-Rule MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LuxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular_function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 168	168	S-ribosylhomocysteine lyase HAMAP-Rule MF_00091		PRO_0000298016
Sites
	Metal binding	54	1	Iron By similarity
	Metal binding	58	1	Iron By similarity
	Metal binding	128	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KW60 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: EF48D9EFDD18854D	FASTA	168	18,683
	10 20 30 40 50 60 MPLLDSFKVD HTRMHAPAVR VAKTMTTPKG DTITVFDLRF CIPNKEILPE KGIHTLEHLF 70 80 90 100 110 120 AGFMRDHLNG NGVEIIDISP MGCRTGFYMS LIGTPSEQQV ADAWLASMQD VGNVKDQSKI 130 140 150 160 PELNEYQCGT YQMHSLAEAQ QIAQNVLARK VAVNKNEELT LDEGLLNA

References

[1]

"Meningococcal genetic variation mechanisms viewed through comparative analysis of serogroup C strain FAM18." Bentley S.D., Vernikos G.S., Snyder L.A.S., Churcher C., Arrowsmith C., Chillingworth T., Cronin A., Davis P.H., Holroyd N.E., Jagels K., Maddison M., Moule S., Rabbinowitsch E., Sharp S., Unwin L., Whitehead S., Quail M.A., Achtman M. , Barrell B.G., Saunders N.J., Parkhill J. PLoS Genet. 3:230-240(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700532 / FAM18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM421808 Genomic DNA. Translation: CAM11116.1.
RefSeq	YP_975884.1. NC_008767.1.
3D structure databases
ProteinModelPortal	A1KW60.
SMR	A1KW60. Positions 3-162.
ModBase	Search...
Protein-protein interaction databases
STRING	272831.NMC1956.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM11116; CAM11116; NMC1956.
GeneID	4676172.
KEGG	nmc:NMC1956.
PATRIC	20354402. VBINeiMen17609_2429.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1854.
HOGENOM	HOG000040371.
KO	K07173.
OMA	KAPYVRV.
ProtClustDB	PRK02260.
Enzyme and pathway databases
BioCyc	NMEN272831:GJDX-1851-MONOMER.
Family and domain databases
Gene3D	3.30.1360.80. 1 hit.
HAMAP	MF_00091. LuxS.
InterPro	IPR011249. Metalloenz_LuxS/M16. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF63411. Metalloenz_metal-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LUXS_NEIMF

Accession

Primary (citable) accession number: A1KW60

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program