Reviewed, UniProtKB/Swiss-Prot A1KU62 (RIBA_NEIMF)

Names and origin

Protein names

Recommended name:
    GTP cyclohydrolase-2
    EC=3.5.4.25
Alternative name(s):
    GTP cyclohydrolase II

Gene names

Name:	ribA
Ordered Locus Names:	NMC1155

Organism

Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) [Complete proteome] [HAMAP]

Taxonomic identifier

272831 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.
Catalytic activity	GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate. HAMAP MF_00179
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 6,7-dimethyl-8-(1-D-ribityl)lumazine from GTP: step 1/4. HAMAP MF_00179
Sequence similarities	Belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase II activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	GTP cyclohydrolase-2 HAMAP MF_00179		PRO_1000040571
Regions
	Nucleotide binding	50 – 54	5	GTP By similarity
	Nucleotide binding	93 – 95	3	GTP By similarity
Sites
	Active site	127	1	Proton acceptor Potential
	Active site	129	1	Nucleophile By similarity
	Metal binding	55	1	Zinc; catalytic By similarity
	Metal binding	66	1	Zinc; catalytic By similarity
	Metal binding	68	1	Zinc; catalytic By similarity
	Binding site	71	1	GTP By similarity
	Binding site	115	1	GTP By similarity
	Binding site	150	1	GTP By similarity
	Binding site	155	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KU62-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: FDC2DA4AF94C61EF	FASTA	197	21,930
	10 20 30 40 50 60 MSEMLNHVAS CRLPTEWGVF TMHGFEEANG QEHVALTVGN FSDGNPVLTR IHSECLTGDA 70 80 90 100 110 120 LFSRKCDCGP QLEAAMRAVQ TEGRGIIVYL RQEGRGIGLI NKIRAYHLQD QGMDTVEANL 130 140 150 160 170 180 ALGLPVDARD FRLAQSIYEY LGIRSVKLLT NNPEKIQTLK DAGINVVERI PLHVGENLEN 190 KRYLQTKADK LGHLMSE

References

[1]

"Meningococcal genetic variation mechanisms viewed through comparative analysis of serogroup C strain FAM18." Bentley S.D., Vernikos G.S., Snyder L.A.S., Churcher C., Arrowsmith C., Chillingworth T., Cronin A., Davis P.H., Holroyd N.E., Jagels K., Maddison M., Moule S., Rabbinowitsch E., Sharp S., Unwin L., Whitehead S., Quail M.A., Achtman M. , Barrell B.G., Saunders N.J., Parkhill J. PLoS Genet. 3:230-240(2007) [PubMed: 17305430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM421808 Genomic DNA. Translation: CAM10403.1.
RefSeq	YP_975191.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4676360.
GenomeReviews	Gene locus NMC1155 in contig AM421808_GR.
KEGG	nmc:NMC1155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A1KU62. QGFILYL.
Family and domain databases
HAMAP	MF_00179. [Tree]
InterPro	IPR000926. GTP_CycHdrlase_II. [Graphical view]
Pfam	PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00505. ribA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBA_NEIMF

Accession

Primary (citable) accession number: A1KU62

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)