Reviewed, UniProtKB/Swiss-Prot A1KU05 (CYSI_NEIMF)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SiR-HP
      Short name=SiRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	NMC1091

Organism

Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) [Complete proteome] [HAMAP]

Taxonomic identifier

272831 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP MF_01540
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. HAMAP MF_01540 Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP MF_01540
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity. HAMAP MF_01540
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_1000068766
Sites
	Metal binding	443	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	449	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	488	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	492	1	Iron (siroheme axial ligand) By similarity
	Metal binding	492	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KU05-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 8659C0997E0CE46B	FASTA	589	65,964
	10 20 30 40 50 60 MTVQTKTKGL AWQEKPLSDN ERLKTESNFL RGTILDDLKD PLTGGFKGDN FQLIRFHGMY 70 80 90 100 110 120 EQDDRDIRAE RAEAKLEPLK FMLLRCRLPG GIIKPSQWIE LDKFARENSH YRSIRLTNRQ 130 140 150 160 170 180 TFQFHGVPKA KLQTMHRLLH KLGLDSIATA ADMNRNVLCT SNPIESELHR QAYEYAKKIS 190 200 210 220 230 240 EHLLPRTRGY LDVWVDGKKV QSSDDFLQED EPILGKTYLP RKFKTAVVIP PLNDVDCYGN 250 260 270 280 290 300 DLDFVAVSDG NGQLAGFNVL AGGGLSMEHG NTKTYPNISL ELGFVPPEHA LKAAEAVVTT 310 320 330 340 350 360 QRDFGNRSDR KNARTRYTIQ NMGLDNFRAE VERRMGMPFE PIRPFKFTGR GDRIGWVKGI 370 380 390 400 410 420 DGNWHLTLFI ESGRLVDEGG KQLLTGVLEI AKIHKGDFRI TANQNLIVAN VAEADKAKIE 430 440 450 460 470 480 ELARTYGLIR NDVSKLRENA MSCVSFPTCP LAMAEAERVL PDFIGELDKI MAKHGTSDDY 490 500 510 520 530 540 IVTRITGCPN GCGRAMLAEI GLVGKAVGRY NLHIGGDREG VRIPRLYKEN ITLPEILSEL 550 560 570 580 DDLIGKWAAG RDTDEGFGDF AIRTGIVKPV LDAPVDFWDA SKAVPIARA

References

[1]

"Meningococcal genetic variation mechanisms viewed through comparative analysis of serogroup C strain FAM18." Bentley S.D., Vernikos G.S., Snyder L.A.S., Churcher C., Arrowsmith C., Chillingworth T., Cronin A., Davis P.H., Holroyd N.E., Jagels K., Maddison M., Moule S., Rabbinowitsch E., Sharp S., Unwin L., Whitehead S., Quail M.A., Achtman M. , Barrell B.G., Saunders N.J., Parkhill J. PLoS Genet. 3:230-240(2007) [PubMed: 17305430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM421808 Genomic DNA. Translation: CAM10346.1.
RefSeq	YP_975134.1.
3D structure databases
SMR	A1KU05. Positions 83-579.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KU05.
Genome annotation databases
GeneID	4676437.
GenomeReviews	Gene locus NMC1091 in contig AM421808_GR.
KEGG	nmc:NMC1091.
NMPDR	fig|487.2.peg.2214.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0155.
HOGENOM	HBG626671.
OMA	IGRYNLH.
PhylomeDB	A1KU05.
Family and domain databases
HAMAP	MF_01540. CysI. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_NEIMF

Accession

Primary (citable) accession number: A1KU05

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)