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Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-isopropylmalate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciNMEN272831:GJDX-999-MONOMER.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
Alternative name(s):
Alpha-IPM synthaseUniRule annotation
Alpha-isopropylmalate synthaseUniRule annotation
Gene namesi
Name:leuAUniRule annotation
Ordered Locus Names:NMC1034
OrganismiNeisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18)
Taxonomic identifieri272831 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000002286 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5175172-isopropylmalate synthasePRO_1000149226Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi272831.NMC1034.

Structurei

3D structure databases

ProteinModelPortaliA1KTV6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1KTV6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQANRVIIF DTTLRDGEQS PGAAMTKEEK IRVARQLEKL GVDIIEAGFA
60 70 80 90 100
AASPGDFEAV NAIAKTITKS TVCSLSRAIE RDIRQAGEAV APAPKKRIHT
110 120 130 140 150
FIATSPIHME YKLKMKPKQV IEAAVKAVKI AREYTDDVEF SCEDALRSEI
160 170 180 190 200
DFLAEICGAV IEAGATTINI PDTVGYSIPY KTEEFFRELI VKTPNGGKVV
210 220 230 240 250
WSAHCHNDLG LAVANSLAAL KGGARQVECT VNGLGERAGN ASVEEIVMAL
260 270 280 290 300
KVRHDLFGLE TGIDTTQIVP SSKLVSTITG YPVQPNKAIV GANAFSHESG
310 320 330 340 350
IHQDGVLKHR ETYEIMSAES VGWATNRLSL GKLSGRNAFK TKLADLGIEL
360 370 380 390 400
ESEEALNAAF ARFKELADKK REIFDEDLHA LVSDEMGSMN AESYKFISQK
410 420 430 440 450
ISTETGEEPR ADIVFSIKGE EKRASATGSG PVDAIFKAIE SVAQSGATLQ
460 470 480 490 500
IYSVNAVTQG TESQGETSVR LARGNRVVNG QGADTDVLVA TAKAYLSALS
510
KLEFSAAKPK AQGSGTI
Length:517
Mass (Da):55,425
Last modified:February 6, 2007 - v1
Checksum:i97E99448542990D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM421808 Genomic DNA. Translation: CAM10296.1.
RefSeqiWP_011798887.1. NC_008767.1.
YP_975085.1. NC_008767.1.

Genome annotation databases

EnsemblBacteriaiCAM10296; CAM10296; NMC1034.
KEGGinmc:NMC1034.
PATRICi20352013. VBINeiMen17609_1259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM421808 Genomic DNA. Translation: CAM10296.1.
RefSeqiWP_011798887.1. NC_008767.1.
YP_975085.1. NC_008767.1.

3D structure databases

ProteinModelPortaliA1KTV6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272831.NMC1034.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM10296; CAM10296; NMC1034.
KEGGinmc:NMC1034.
PATRICi20352013. VBINeiMen17609_1259.

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070.
BioCyciNMEN272831:GJDX-999-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700532 / DSM 15464 / FAM18.

Entry informationi

Entry nameiLEU1_NEIMF
AccessioniPrimary (citable) accession number: A1KTV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: April 29, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.