A1KTV6 (LEU1_NEIMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-isopropylmalate synthase
EC=2.3.3.13

Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase

Gene names

Name:	leuA
Ordered Locus Names:	NMC1034

Organism

Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) [Complete proteome] [HAMAP]

Taxonomic identifier

272831 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria meningitidis › Neisseria meningitidis serogroup C

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_01025
Catalytic activity	Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_01025
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_01025
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-isopropylmalate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	2-isopropylmalate synthase HAMAP-Rule MF_01025		PRO_1000149226

Sequences

	Sequence		Length	Mass (Da)
	A1KTV6 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 97E99448542990D6	FASTA	517	55,425
	10 20 30 40 50 60 MTQANRVIIF DTTLRDGEQS PGAAMTKEEK IRVARQLEKL GVDIIEAGFA AASPGDFEAV 70 80 90 100 110 120 NAIAKTITKS TVCSLSRAIE RDIRQAGEAV APAPKKRIHT FIATSPIHME YKLKMKPKQV 130 140 150 160 170 180 IEAAVKAVKI AREYTDDVEF SCEDALRSEI DFLAEICGAV IEAGATTINI PDTVGYSIPY 190 200 210 220 230 240 KTEEFFRELI VKTPNGGKVV WSAHCHNDLG LAVANSLAAL KGGARQVECT VNGLGERAGN 250 260 270 280 290 300 ASVEEIVMAL KVRHDLFGLE TGIDTTQIVP SSKLVSTITG YPVQPNKAIV GANAFSHESG 310 320 330 340 350 360 IHQDGVLKHR ETYEIMSAES VGWATNRLSL GKLSGRNAFK TKLADLGIEL ESEEALNAAF 370 380 390 400 410 420 ARFKELADKK REIFDEDLHA LVSDEMGSMN AESYKFISQK ISTETGEEPR ADIVFSIKGE 430 440 450 460 470 480 EKRASATGSG PVDAIFKAIE SVAQSGATLQ IYSVNAVTQG TESQGETSVR LARGNRVVNG 490 500 510 QGADTDVLVA TAKAYLSALS KLEFSAAKPK AQGSGTI

References

[1]

"Meningococcal genetic variation mechanisms viewed through comparative analysis of serogroup C strain FAM18." Bentley S.D., Vernikos G.S., Snyder L.A.S., Churcher C., Arrowsmith C., Chillingworth T., Cronin A., Davis P.H., Holroyd N.E., Jagels K., Maddison M., Moule S., Rabbinowitsch E., Sharp S., Unwin L., Whitehead S., Quail M.A., Achtman M. , Barrell B.G., Saunders N.J., Parkhill J. PLoS Genet. 3:230-240(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700532 / FAM18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM421808 Genomic DNA. Translation: CAM10296.1.
RefSeq	YP_975085.1. NC_008767.1.
3D structure databases
ProteinModelPortal	A1KTV6.
ModBase	Search...
Protein-protein interaction databases
STRING	272831.NMC1034.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM10296; CAM10296; NMC1034.
GeneID	4675871.
KEGG	nmc:NMC1034.
PATRIC	20352013. VBINeiMen17609_1259.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0119.
HOGENOM	HOG000046859.
KO	K01649.
OMA	VWSVHCH.
ProtClustDB	PRK00915.
Enzyme and pathway databases
BioCyc	NMEN272831:GJDX-999-MONOMER.
UniPathway	UPA00048; UER00070.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01025. LeuA_type1.
InterPro	IPR013709. 2-isopropylmalate_synth_dimer. IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR005671. LeuA_bact_synth. IPR000891. PYR_CT. [Graphical view]
Pfam	PF00682. HMGL-like. 1 hit. PF08502. LeuA_dimer. 1 hit. [Graphical view]
SMART	SM00917. LeuA_dimer. 1 hit. [Graphical view]
SUPFAM	SSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMs	TIGR00973. leuA_bact. 1 hit.
PROSITE	PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit. PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU1_NEIMF

Accession

Primary (citable) accession number: A1KTV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program