A1KTL3 (METE_NEIMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	NMC0922

Organism

Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / FAM18) [Complete proteome] [HAMAP]

Taxonomic identifier

272831 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017258
Sites
	Metal binding	643	1	Zinc By similarity
	Metal binding	645	1	Zinc By similarity
	Metal binding	728	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KTL3 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 89E2D3B1AF4E1312	FASTA	758	85,147
	10 20 30 40 50 60 MTTLHFSGFP RVGAFRELKF AQEKYWRKEI SEQELLAVAK DLREKNWKHQ AAANADFVAV 70 80 90 100 110 120 GDFTFYDHIL DLQVATGAIP ARFGFDSQNL SLEQFFQLAR GNKDQFAIEM TKWFDTNYHY 130 140 150 160 170 180 LVPEFHADTE FKANAKHYVQ QLQEAQALGL KAKPTVVGPL TFLWVGKEKG TVEFNRLSLL 190 200 210 220 230 240 QKLLPVYVEI LNALVEAGAE WIQIDEPALT VDLPKEWVEA YKDVYATLSK VSAKILLSTY 250 260 270 280 290 300 FGSVAEHAAL LKSLPVDGLH IDLVRAPEQL DAFADYDKVL SAGIIDGRNI WRANLNKVLE 310 320 330 340 350 360 TVEPLQAKLG DRLWISSSCS LLHTPFDLSV EEKLKANKPD LYSWLAFTLQ KTQELRVLKA 370 380 390 400 410 420 ALNEGRDSVA EELAASQAAA DSRANSSEIH RADVAKRLAD LPANADQRKS PFADRIKAQQ 430 440 450 460 470 480 AWLNLPLLPT TNIGSFPQTT EIRQARAAFK KGELSAADYE AAMKKEIALV VEEQEKLDLD 490 500 510 520 530 540 VLVHGEAERN DMVEYFGELL SGFAFTQYGW VQSYGSRCVK PPIIFGDVSR PEAMTVAWST 550 560 570 580 590 600 YAQSLTKRPM KGMLTGPVTI LQWSFVRNDI PRSTVCKQIA LALNDEVLDL EKAGIKVIQI 610 620 630 640 650 660 DEPAIREGLP LKRADWDAYL NWAGESFRLS SAGCEDSTQI HTHMCYSEFN DILPAIAAMD 670 680 690 700 710 720 ADVITIETSR SDMELLTAFG EFKYPNDIGP GVYDIHSPRV PTEAEVEHLL RKAIEVVPVE 730 740 750 RLWVNPDCGL KTRGWKETLE QLQVMMNVTH KLRAELAK

References

[1]

"Meningococcal genetic variation mechanisms viewed through comparative analysis of serogroup C strain FAM18." Bentley S.D., Vernikos G.S., Snyder L.A.S., Churcher C., Arrowsmith C., Chillingworth T., Cronin A., Davis P.H., Holroyd N.E., Jagels K., Maddison M., Moule S., Rabbinowitsch E., Sharp S., Unwin L., Whitehead S., Quail M.A., Achtman M. , Barrell B.G., Saunders N.J., Parkhill J. PLoS Genet. 3:230-240(2007) [PubMed: 17305430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700532 / FAM18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM421808 Genomic DNA. Translation: CAM10199.1.
RefSeq	YP_974992.1. NC_008767.1.
3D structure databases
ProteinModelPortal	A1KTL3.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KTL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000007579; EBNEIP00000007348; EBNEIG00000007579.
GeneID	4676314.
GenomeReviews	Gene locus NMC0922 in contig AM421808_GR.
KEGG	nmc:NMC0922.
NMPDR	fig|487.2.peg.2287.
PATRIC	20351753. VBINeiMen17609_1124.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
GeneTree	EBGT00050000021045.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	A1KTL3.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	NMEN272831:NMC0922-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_NEIMF

Accession

Primary (citable) accession number: A1KTL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program