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Protein

UDP-2,3-diacylglucosamine hydrolase

Gene

lpxH

Organism
Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP by catalyzing the attack of water at the alpha-P atom. Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + H2O = 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate + UMP.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 Mn2+ ions per subunit in a binuclear metal center.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Manganese 1UniRule annotation1
Metal bindingi11Manganese 1; via tele nitrogenUniRule annotation1
Metal bindingi43Manganese 1UniRule annotation1
Metal bindingi43Manganese 2UniRule annotation1
Metal bindingi81Manganese 2UniRule annotation1
Metal bindingi116Manganese 2; via tele nitrogenUniRule annotation1
Binding sitei124SubstrateUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Binding sitei166SubstrateUniRule annotation1
Binding sitei169SubstrateUniRule annotation1
Metal bindingi197Manganese 2; via pros nitrogenUniRule annotation1
Binding sitei197Substrate; via tele nitrogenUniRule annotation1
Metal bindingi199Manganese 1; via tele nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandManganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00359; UER00480.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-2,3-diacylglucosamine hydrolaseUniRule annotation (EC:3.6.1.54UniRule annotation)
Alternative name(s):
UDP-2,3-diacylglucosamine diphosphataseUniRule annotation
Gene namesi
Name:lpxHUniRule annotation
Ordered Locus Names:NMC0483
OrganismiNeisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18)
Taxonomic identifieri272831 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000002286 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000250641 – 240UDP-2,3-diacylglucosamine hydrolaseAdd BLAST240

Structurei

3D structure databases

SMRiA1KSF3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni81 – 82Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the LpxH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000261930.
KOiK03269.
OMAiFDFWFEY.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
HAMAPiMF_00575. LpxH. 1 hit.
InterProiView protein in InterPro
IPR024654. Calcineurin-like_PHP_lpxH.
IPR029052. Metallo-depent_PP-like.
IPR010138. UDP-diacylglucosamine_Hdrlase.
PANTHERiPTHR34990:SF3. PTHR34990:SF3. 1 hit.
PfamiView protein in Pfam
PF12850. Metallophos_2. 1 hit.
SUPFAMiSSF56300. SSF56300. 2 hits.
TIGRFAMsiTIGR01854. lipid_A_lpxH. 1 hit.

Sequencei

Sequence statusi: Complete.

A1KSF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPAYFISDL HLSEKHPELT ALLLRFLRSS AAGQARAVYI LGDLFDFWVG
60 70 80 90 100
DDEVSELNTS VAREIRKLSD KGVAVFFVRG NRDFLIGQDF CRQAGMTLLP
110 120 130 140 150
DYSVLDLFGC KTLICHGDTL CTDDRAYQRF RKIVHRKRLQ KLFLMLPLKW
160 170 180 190 200
RTRLATKIRR VSKMEKQVKP ADIMDVNAAF TARQVRAFGA ERLIHGHTHR
210 220 230 240
EHIHHENGFT RIVLGDWHND YASILRVDGD GAVFVPLEKY
Length:240
Mass (Da):27,656
Last modified:February 6, 2007 - v1
Checksum:i217A8868E8894227
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM421808 Genomic DNA. Translation: CAM09783.1.
RefSeqiWP_002221383.1. NC_008767.1.

Genome annotation databases

EnsemblBacteriaiCAM09783; CAM09783; NMC0483.
KEGGinmc:NMC0483.

Similar proteinsi

Entry informationi

Entry nameiLPXH_NEIMF
AccessioniPrimary (citable) accession number: A1KSF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: August 30, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families