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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi892 – 8921ZincUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi912 – 9121ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNMEN272831:GJDX-382-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NMC0383
OrganismiNeisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18)
Taxonomic identifieri272831 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000002286 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Isoleucine--tRNA ligasePRO_1000022093Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA1KS62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1KS62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYSKTVNL LESPFPMRGN LAKREPAWLK SWYEQKRYQK LREIAKGRPK
60 70 80 90 100
FILHDGPPYA NGDIHIGHAV NKILKDIIIR SKTQAGFDAP YVPGWDCHGL
110 120 130 140 150
PIEVMVEKLH GKDMPKARFR ELCREYAAEQ VACQKKDFIR LGVLGDWDKP
160 170 180 190 200
YLTMDFKTEA DTVRMLGEIY KSGYLYRGAK PVQFCLDCGS SLAEAEVEYK
210 220 230 240 250
DKVSPAIDVG YPFKDTAALA AAFGLAGIEG KAFAVIWTTT PWTLPASQAV
260 270 280 290 300
SAGADVVYQL IDTPKGKLVL AKDLAEDALK RYGFSDGIAI LAETTGDKLE
310 320 330 340 350
NLHMNHPFLE RDIPMLNGDH VTTDAGTGLV HTAPAHGLED YAVCNKYGIE
360 370 380 390 400
LYNPVNAEGK YISETPRVAG MSVWEANPVI LQWLEETGNL LASSKIEHSY
410 420 430 440 450
AHCWRHKTPL IYRATGQWFV GMDKAGADGK TLRDKAIKAV DDTEFFPSWG
460 470 480 490 500
RARLEAMIEG RPDWVVSRQR YWGTPMTFFV HKETGELHPN SAELLEKVAQ
510 520 530 540 550
RIEEKGIEAW FSLDKGELLS AEDCEHYDKL SDTMDVWFDS GSTHYSVVKQ
560 570 580 590 600
REELDWPADL YLEGSDQHRG WFQSSMLTGC ASSMGRAPYK QLLTHGFVVD
610 620 630 640 650
QNGRKMSKSI GNVVAPQEVY NEFGADILRL WAASTDYSGE LAISKEILKR
660 670 680 690 700
VTESYRRIRN TLSFLFANLS DFNPIEDAVQ QADMVEIDRY AVVLARQLQE
710 720 730 740 750
RLAGDYYPRY TFHFAVKDIV SFCSEDLGAF YLDILKDRLY TTKADSRARR
760 770 780 790 800
SAQTALYHIT RSLVLLIAPI LCFTGEEAWD IIGGGEEDSV LFHTWHEFPT
810 820 830 840 850
INEKAEAELV KKWTAIREAR EAVTAAIEPL RADKTVGSSL QAEAEITAPE
860 870 880 890 900
EMAGYLNALG EELRFALLVS KAEVKVGSEL AVAAKASDGE KCERCWHYTR
910 920
DVGAVAGYET VCKRCAENVG REGETRHYA
Length:929
Mass (Da):104,241
Last modified:February 6, 2007 - v1
Checksum:i0E17FA28C7261036
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM421808 Genomic DNA. Translation: CAM09692.1.
RefSeqiWP_002221500.1. NC_008767.1.
YP_974494.1. NC_008767.1.

Genome annotation databases

EnsemblBacteriaiCAM09692; CAM09692; NMC0383.
KEGGinmc:NMC0383.
PATRICi20350409. VBINeiMen17609_0462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM421808 Genomic DNA. Translation: CAM09692.1.
RefSeqiWP_002221500.1. NC_008767.1.
YP_974494.1. NC_008767.1.

3D structure databases

ProteinModelPortaliA1KS62.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM09692; CAM09692; NMC0383.
KEGGinmc:NMC0383.
PATRICi20350409. VBINeiMen17609_0462.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciNMEN272831:GJDX-382-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700532 / DSM 15464 / FAM18.

Entry informationi

Entry nameiSYI_NEIMF
AccessioniPrimary (citable) accession number: A1KS62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: June 24, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.