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Protein

Diacylglycerol acyltransferase/mycolyltransferase Ag85A

Gene

fbpA

Organism
Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA (By similarity).By similarity

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei169 – 1691NucleophileBy similarity
Binding sitei169 – 1691SubstrateBy similarity
Binding sitei197 – 1971SubstrateBy similarity
Active sitei273 – 2731By similarity
Binding sitei282 – 2821SubstrateBy similarity
Active sitei305 – 3051By similarity

GO - Molecular functioni

  1. diacylglycerol O-acyltransferase activity Source: UniProtKB-EC
  2. trehalose O-mycolyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciMBOV410289:GJW7-3917-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol acyltransferase/mycolyltransferase Ag85A (EC:2.3.1.122, EC:2.3.1.20)
Short name:
DGAT
Alternative name(s):
Acyl-CoA:diacylglycerol acyltransferase
Antigen 85 complex A
Short name:
85A
Short name:
Ag85A
Fibronectin-binding protein A
Short name:
Fbps A
Gene namesi
Name:fbpA
Synonyms:mpt44
Ordered Locus Names:BCG_3866c
OrganismiMycobacterium bovis (strain BCG / Pasteur 1173P2)
Taxonomic identifieri410289 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001472: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-SubCell
  3. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4242By similarityAdd
BLAST
Chaini43 – 338296Diacylglycerol acyltransferase/mycolyltransferase Ag85APRO_0000285177Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi130 ↔ 135By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi410289.BCG_3866c.

Structurei

3D structure databases

ProteinModelPortaliA1KQD8.
SMRiA1KQD8. Positions 43-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni85 – 862Substrate bindingBy similarity
Regioni101 – 11111Fibronectin-bindingAdd
BLAST
Regioni275 – 2784Substrate bindingBy similarity
Regioni305 – 3073Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the mycobacterial A85 antigen family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0627.
HOGENOMiHOG000049505.
OMAiGGHNAVW.
OrthoDBiEOG6CVV8G.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1KQD8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQLVDRVRGA VTGMSRRLVV GAVGAALVSG LVGAVGGTAT AGAFSRPGLP
60 70 80 90 100
VEYLQVPSPS MGRDIKVQFQ SGGANSPALY LLDGLRAQDD FSGWDINTPA
110 120 130 140 150
FEWYDQSGLS VVMPVGGQSS FYSDWYQPAC GKAGCQTYKW ETFLTSELPG
160 170 180 190 200
WLQANRHVKP TGSAVVGLSM AASSALTLAI YHPQQFVYAG AMSGLLDPSQ
210 220 230 240 250
AMGPTLIGLA MGDAGGYKAS DMWGPKEDPA WQRNDPLLNV GKLIANNTRV
260 270 280 290 300
WVYCGNGKPS DLGGNNLPAK FLEGFVRTSN IKFQDAYNAG GGHNGVFDFP
310 320 330
DSGTHSWEYW GAQLNAMKPD LQRALGATPN TGPAPQGA
Length:338
Mass (Da):35,686
Last modified:February 6, 2007 - v1
Checksum:i57B1CF95D07D52C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53034 Genomic DNA. Translation: CAA37206.1.
AM408590 Genomic DNA. Translation: CAL73856.1.
PIRiS10326.
RefSeqiYP_979945.1. NC_008769.1.

Genome annotation databases

EnsemblBacteriaiCAL73856; CAL73856; BCG_3866c.
GeneIDi4696523.
KEGGimbb:BCG_3866c.
PATRICi18018927. VBIMycBov80988_4198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53034 Genomic DNA. Translation: CAA37206.1.
AM408590 Genomic DNA. Translation: CAL73856.1.
PIRiS10326.
RefSeqiYP_979945.1. NC_008769.1.

3D structure databases

ProteinModelPortaliA1KQD8.
SMRiA1KQD8. Positions 43-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi410289.BCG_3866c.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL73856; CAL73856; BCG_3866c.
GeneIDi4696523.
KEGGimbb:BCG_3866c.
PATRICi18018927. VBIMycBov80988_4198.

Phylogenomic databases

eggNOGiCOG0627.
HOGENOMiHOG000049505.
OMAiGGHNAVW.
OrthoDBiEOG6CVV8G.

Enzyme and pathway databases

BioCyciMBOV410289:GJW7-3917-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the 32 kDa-protein gene (antigen 85 A) of Mycobacterium bovis BCG."
    de Wit L., de la Cuvellerie A., Ooms J., Content J.
    Nucleic Acids Res. 18:3995-3995(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BCG / Pasteur 1173P2.

Entry informationi

Entry nameiA85A_MYCBP
AccessioniPrimary (citable) accession number: A1KQD8
Secondary accession number(s): P0A4V3, P17944, P17996
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 6, 2007
Last modified: January 7, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.