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A1KQD8

- A85A_MYCBP

UniProt

A1KQD8 - A85A_MYCBP

Protein

Diacylglycerol acyltransferase/mycolyltransferase Ag85A

Gene

fbpA

Organism
Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA By similarity.By similarity

    Catalytic activityi

    Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
    2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei169 – 1691NucleophileBy similarity
    Binding sitei169 – 1691SubstrateBy similarity
    Binding sitei197 – 1971SubstrateBy similarity
    Active sitei273 – 2731By similarity
    Binding sitei282 – 2821SubstrateBy similarity
    Active sitei305 – 3051By similarity

    GO - Molecular functioni

    1. diacylglycerol O-acyltransferase activity Source: UniProtKB-EC
    2. trehalose O-mycolyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMBOV410289:GJW7-3917-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol acyltransferase/mycolyltransferase Ag85A (EC:2.3.1.122, EC:2.3.1.20)
    Short name:
    DGAT
    Alternative name(s):
    Acyl-CoA:diacylglycerol acyltransferase
    Antigen 85 complex A
    Short name:
    85A
    Short name:
    Ag85A
    Fibronectin-binding protein A
    Short name:
    Fbps A
    Gene namesi
    Name:fbpA
    Synonyms:mpt44
    Ordered Locus Names:BCG_3866c
    OrganismiMycobacterium bovis (strain BCG / Pasteur 1173P2)
    Taxonomic identifieri410289 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001472: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-SubCell
    3. extracellular region Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell wall, Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4242By similarityAdd
    BLAST
    Chaini43 – 338296Diacylglycerol acyltransferase/mycolyltransferase Ag85APRO_0000285177Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi130 ↔ 135By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi410289.BCG_3866c.

    Structurei

    3D structure databases

    ProteinModelPortaliA1KQD8.
    SMRiA1KQD8. Positions 43-330.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni85 – 862Substrate bindingBy similarity
    Regioni101 – 11111Fibronectin-bindingAdd
    BLAST
    Regioni275 – 2784Substrate bindingBy similarity
    Regioni305 – 3073Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the mycobacterial A85 antigen family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0627.
    HOGENOMiHOG000049505.
    OMAiGGHNAVW.
    OrthoDBiEOG6CVV8G.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000801. Esterase_put.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF00756. Esterase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1KQD8-1 [UniParc]FASTAAdd to Basket

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    MQLVDRVRGA VTGMSRRLVV GAVGAALVSG LVGAVGGTAT AGAFSRPGLP    50
    VEYLQVPSPS MGRDIKVQFQ SGGANSPALY LLDGLRAQDD FSGWDINTPA 100
    FEWYDQSGLS VVMPVGGQSS FYSDWYQPAC GKAGCQTYKW ETFLTSELPG 150
    WLQANRHVKP TGSAVVGLSM AASSALTLAI YHPQQFVYAG AMSGLLDPSQ 200
    AMGPTLIGLA MGDAGGYKAS DMWGPKEDPA WQRNDPLLNV GKLIANNTRV 250
    WVYCGNGKPS DLGGNNLPAK FLEGFVRTSN IKFQDAYNAG GGHNGVFDFP 300
    DSGTHSWEYW GAQLNAMKPD LQRALGATPN TGPAPQGA 338
    Length:338
    Mass (Da):35,686
    Last modified:February 6, 2007 - v1
    Checksum:i57B1CF95D07D52C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53034 Genomic DNA. Translation: CAA37206.1.
    AM408590 Genomic DNA. Translation: CAL73856.1.
    PIRiS10326.
    RefSeqiYP_979945.1. NC_008769.1.

    Genome annotation databases

    EnsemblBacteriaiCAL73856; CAL73856; BCG_3866c.
    GeneIDi4696523.
    KEGGimbb:BCG_3866c.
    PATRICi18018927. VBIMycBov80988_4198.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53034 Genomic DNA. Translation: CAA37206.1 .
    AM408590 Genomic DNA. Translation: CAL73856.1 .
    PIRi S10326.
    RefSeqi YP_979945.1. NC_008769.1.

    3D structure databases

    ProteinModelPortali A1KQD8.
    SMRi A1KQD8. Positions 43-330.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 410289.BCG_3866c.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL73856 ; CAL73856 ; BCG_3866c .
    GeneIDi 4696523.
    KEGGi mbb:BCG_3866c.
    PATRICi 18018927. VBIMycBov80988_4198.

    Phylogenomic databases

    eggNOGi COG0627.
    HOGENOMi HOG000049505.
    OMAi GGHNAVW.
    OrthoDBi EOG6CVV8G.

    Enzyme and pathway databases

    BioCyci MBOV410289:GJW7-3917-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000801. Esterase_put.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF00756. Esterase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the 32 kDa-protein gene (antigen 85 A) of Mycobacterium bovis BCG."
      de Wit L., de la Cuvellerie A., Ooms J., Content J.
      Nucleic Acids Res. 18:3995-3995(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BCG / Pasteur 1173P2.

    Entry informationi

    Entry nameiA85A_MYCBP
    AccessioniPrimary (citable) accession number: A1KQD8
    Secondary accession number(s): P0A4V3, P17944, P17996
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3