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A1KQD8 (A85A_MYCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diacylglycerol acyltransferase/mycolyltransferase Ag85A

Short name=DGAT
EC=2.3.1.122
EC=2.3.1.20
Alternative name(s):
Acyl-CoA:diacylglycerol acyltransferase
Antigen 85 complex A
Short name=85A
Short name=Ag85A
Fibronectin-binding protein A
Short name=Fbps A
Gene names
Name:fbpA
Synonyms:mpt44
Ordered Locus Names:BCG_3866c
OrganismMycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]
Taxonomic identifier410289 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA By similarity.

Catalytic activity

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate.

Subunit structure

Homodimer By similarity.

Subcellular location

Secretedcell wall. Cytoplasm.

Sequence similarities

Belongs to the mycobacterial A85 antigen family.

Ontologies

Keywords
   Cellular componentCell wall
Cytoplasm
Secreted
   DomainSignal
   Molecular functionAcyltransferase
Transferase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiondiacylglycerol O-acyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

trehalose O-mycolyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4242 By similarity
Chain43 – 338296Diacylglycerol acyltransferase/mycolyltransferase Ag85A
PRO_0000285177

Regions

Region85 – 862Substrate binding By similarity
Region101 – 11111Fibronectin-binding
Region275 – 2784Substrate binding By similarity
Region305 – 3073Substrate binding By similarity

Sites

Active site1691Nucleophile By similarity
Active site2731 By similarity
Active site3051 By similarity
Binding site1691Substrate By similarity
Binding site1971Substrate By similarity
Binding site2821Substrate By similarity

Amino acid modifications

Disulfide bond130 ↔ 135 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1KQD8 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 57B1CF95D07D52C0

FASTA33835,686
        10         20         30         40         50         60 
MQLVDRVRGA VTGMSRRLVV GAVGAALVSG LVGAVGGTAT AGAFSRPGLP VEYLQVPSPS 

        70         80         90        100        110        120 
MGRDIKVQFQ SGGANSPALY LLDGLRAQDD FSGWDINTPA FEWYDQSGLS VVMPVGGQSS 

       130        140        150        160        170        180 
FYSDWYQPAC GKAGCQTYKW ETFLTSELPG WLQANRHVKP TGSAVVGLSM AASSALTLAI 

       190        200        210        220        230        240 
YHPQQFVYAG AMSGLLDPSQ AMGPTLIGLA MGDAGGYKAS DMWGPKEDPA WQRNDPLLNV 

       250        260        270        280        290        300 
GKLIANNTRV WVYCGNGKPS DLGGNNLPAK FLEGFVRTSN IKFQDAYNAG GGHNGVFDFP 

       310        320        330 
DSGTHSWEYW GAQLNAMKPD LQRALGATPN TGPAPQGA 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the 32 kDa-protein gene (antigen 85 A) of Mycobacterium bovis BCG."
de Wit L., de la Cuvellerie A., Ooms J., Content J.
Nucleic Acids Res. 18:3995-3995(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genome plasticity of BCG and impact on vaccine efficacy."
Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T.
Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BCG / Pasteur 1173P2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53034 Genomic DNA. Translation: CAA37206.1.
AM408590 Genomic DNA. Translation: CAL73856.1.
PIRS10326.
RefSeqYP_979945.1. NC_008769.1.

3D structure databases

ProteinModelPortalA1KQD8.
SMRA1KQD8. Positions 43-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING410289.BCG_3866c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL73856; CAL73856; BCG_3866c.
GeneID4696523.
KEGGmbb:BCG_3866c.
PATRIC18018927. VBIMycBov80988_4198.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0627.
HOGENOMHOG000049505.
OMAGGHNAVW.
OrthoDBEOG6CVV8G.

Enzyme and pathway databases

BioCycMBOV410289:GJW7-3917-MONOMER.

Family and domain databases

InterProIPR000801. Esterase_put.
IPR006311. TAT_signal.
[Graphical view]
PfamPF00756. Esterase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA85A_MYCBP
AccessionPrimary (citable) accession number: A1KQD8
Secondary accession number(s): P0A4V3, P17944, P17996
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families