A1KP72 (A1KP72_MYCBP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 HAMAP MF_00191
EC=1.17.1.2 HAMAP MF_00191

Gene names

Name:	lytB1 EMBL CAL73440.1
Synonyms:	ispH2 HAMAP MF_00191
Ordered Locus Names:	BCG_3451c

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191 SAAS SAAS003451
Catalytic activity	Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 SAAS SAAS003451 Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 SAAS SAAS003451
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 SAAS SAAS003451 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191 SAAS SAAS003451
Sequence similarities	Belongs to the IspH family. HAMAP MF_00191

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00191 SAAS SAAS003451
Ligand	3Fe-4S HAMAP MF_00191 SAAS SAAS003451 Iron Iron-sulfur Metal-binding NADP HAMAP MF_00191 SAAS SAAS003451
Molecular function	Oxidoreductase HAMAP MF_00191 SAAS SAAS003451
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A1KP72 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 00540C5C87B2AB6B	FASTA	329	34,666
	10 20 30 40 50 60 MAEVFVGPVA QGYASGEVTV LLASPRSFCA GVERAIETVK RVLDVAEGPV YVRKQIVHNT 70 80 90 100 110 120 VVVAELRDRG AVFVEDLDEI PDPPPPGAVV VFSAHGVSPA VRAGADERGL QVVDATCPLV 130 140 150 160 170 180 AKVHAEAARF AARGDTVVFI GHAGHEETEG TLGVAPRSTL LVQTPADVAA LNLPEGTQLS 190 200 210 220 230 240 YLTQTTLALD ETADVIDALR ARFPTLGQPP SEDICYATTN RQRALQSMVG ECDVVLVIGS 250 260 270 280 290 300 CNSSNSRRLV ELAQRSGTPA YLIDGPDDIE PEWLSSVSTI GVTAGASAPP RLVGQVIDAL 310 320 RGYASITVVE RSIATETVRF GLPKQVRAQ

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed: 17372194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL73440.1.
RefSeq	YP_979530.1. NC_008769.1.
3D structure databases
ProteinModelPortal	A1KP72.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KP72.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000021170; EBMYCP00000020920; EBMYCG00000021165.
GeneID	4696508.
GenomeReviews	Gene locus BCG_3451c in contig AM408590_GR.
KEGG	mbb:BCG_3451c.
PATRIC	18018040. VBIMycBov80988_3762.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
GeneTree	EBGT00050000016858.
HOGENOM	HBG335228.
OMA	HNQGKRY.
ProtClustDB	CLSK799986.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A1KP72_MYCBP

Accession

Primary (citable) accession number: A1KP72

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)