A1KMX0 (UNG_MYCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil-DNA glycosylase
Short name=UDG
EC=3.2.2.27

Gene names

Name:	ung
Ordered Locus Names:	BCG_2997c

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis › Mycobacterium bovis BCG

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine By similarity. HAMAP-Rule MF_00148
Catalytic activity	Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil. HAMAP-Rule MF_00148
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the uracil-DNA glycosylase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	uracil DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Uracil-DNA glycosylase HAMAP-Rule MF_00148		PRO_1000009911
Sites
	Active site	68	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KMX0 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 379B5DB72894A304	FASTA	227	24,481
	10 20 30 40 50 60 MTARPLSELV ERGWAAALEP VADQVAHMGQ FLRAEIAAGR RYLPAGSNVL RAFTFPFDNV 70 80 90 100 110 120 RVLIVGQDPY PTPGHAVGLS FSVAPDVRPW PRSLANIFDE YTADLGYPLP SNGDLTPWAQ 130 140 150 160 170 180 RGVLLLNRVL TVRPSNPASH RGKGWEAVTE CAIRALAARA APLVAILWGR DASTLKPMLA 190 200 210 220 AGNCVAIESP HPSPLSASRG FFGSRPFSRA NELLVGMGAE PIDWRLP

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BCG / Pasteur 1173P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL72986.1.
RefSeq	YP_979081.1. NC_008769.1.
3D structure databases
ProteinModelPortal	A1KMX0.
SMR	A1KMX0. Positions 1-227.
ModBase	Search...
Protein-protein interaction databases
STRING	410289.BCG_2997c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL72986; CAL72986; BCG_2997c.
GeneID	4698022.
KEGG	mbb:BCG_2997c.
PATRIC	18017048. VBIMycBov80988_3270.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0692.
HOGENOM	HOG000229528.
KO	K03648.
OMA	KGWEEVT.
ProtClustDB	PRK05254.
Enzyme and pathway databases
BioCyc	MBOV410289:GJW7-3037-MONOMER.
Family and domain databases
Gene3D	3.40.470.10. 1 hit.
HAMAP	MF_00148. UDG.
InterPro	IPR018085. Ura-DNA_Glyclase_AS. IPR002043. Ura_DNA_glycsylse. IPR005122. Uracil-DNA_glycosylase-like. [Graphical view]
PANTHER	PTHR11264. PTHR11264. 1 hit.
Pfam	PF03167. UDG. 1 hit. [Graphical view]
SMART	SM00986. UDG. 1 hit. [Graphical view]
SUPFAM	SSF52141. UDNA_glycsylseSF. 1 hit.
TIGRFAMs	TIGR00628. ung. 1 hit.
PROSITE	PS00130. U_DNA_GLYCOSYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UNG_MYCBP

Accession

Primary (citable) accession number: A1KMX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program