A1KM94 (DAPA_MYCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	BCG_2769c

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050226
Regions
	Region	58 – 59	2	Pyruvate binding By similarity
Sites
	Active site	171	1	Schiff-base intermediate with substrate By similarity
	Binding site	116	1	Pyruvate By similarity
	Site	143	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KM94 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 71CB322661416367	FASTA	300	30,858
	10 20 30 40 50 60 MTTVGFDVAA RLGTLLTAMV TPFSGDGSLD TATAARLANH LVDQGCDGLV VSGTTGESPT 70 80 90 100 110 120 TTDGEKIELL RAVLEAVGDR ARVIAGAGTY DTAHSIRLAK ACAAEGAHGL LVVTPYYSKP 130 140 150 160 170 180 PQRGLQAHFT AVADATELPM LLYDIPGRSA VPIEPDTIRA LASHPNIVGV KDAKADLHSG 190 200 210 220 230 240 AQIMADTGLA YYSGDDALNL PWLAMGATGF ISVIAHLAAG QLRELLSAFG SGDIATARKI 250 260 270 280 290 300 NIAVAPLCNA MSRLGGVTLS KAGLRLQGID VGDPRLPQVA ATPEQIDALA ADMRAASVLR

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed: 17372194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BCG / Pasteur 1173P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL72757.1.
RefSeq	YP_978855.1. NC_008769.1.
3D structure databases
ProteinModelPortal	A1KM94.
SMR	A1KM94. Positions 5-300.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KM94.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000021966; EBMYCP00000021716; EBMYCG00000021961.
GeneID	4699062.
GenomeReviews	Gene locus BCG_2769c in contig AM408590_GR.
KEGG	mbb:BCG_2769c.
PATRIC	18016536. VBIMycBov80988_3014.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
GeneTree	EBGT00050000016215.
HOGENOM	HBG358848.
OMA	HGCISVV.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	MBOV410289:BCG_2769C-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_MYCBP

Accession

Primary (citable) accession number: A1KM94

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program