A1KM51 (STHA_MYCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble pyridine nucleotide transhydrogenase
Short name=STH
EC=1.6.1.1

Alternative name(s):
NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Ordered Locus Names:	BCG_2726

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity. HAMAP MF_00247
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit By similarity. HAMAP MF_00247
Subcellular location	Cytoplasm By similarity HAMAP MF_00247.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_1000012559
Regions
	Nucleotide binding	33 – 42	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KM51 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: D9E737C41C2898CF	FASTA	468	50,754
	10 20 30 40 50 60 MREYDIVVIG SGPGGQKAAI ASAKLGKSVA IVERGRMLGG VCVNTGTIPS KTLREAVLYL 70 80 90 100 110 120 TGMNQRELYG ASYRVKDRIT PADLLARTQH VIGKEVDVVR NQLMRNRVDL IVGHGRFIDP 130 140 150 160 170 180 HTILVEDQAR REKTTVTGDY IIIATGTRPA RPSGVEFDEE RVLDSDGILD LKSLPSSMVV 190 200 210 220 230 240 VGAGVIGIEY ASMFAALGTK VTVVEKRDNM LDFCDPEVVE ALKFHLRDLA VTFRFGEEVT 250 260 270 280 290 300 AVDVGSAGTV TTLASGKQIP AETVMYSAGR QGQTDHLDLH NAGLEVQGRG RIFVDDRFQT 310 320 330 340 350 360 KVDHIYAVGD VIGFPALAAT SMEQGRLAAY HAFGEPTDGI TELQPIGIYS IPEVSYVGAT 370 380 390 400 410 420 EVELTKSSIP YEVGVARYRE LARGQIAGDS YGMLKLLVST EDLKLLGVHI FGTSATEMVH 430 440 450 460 IGQAVMGCGG SVEYLVDAVF NYPTFSEAYK NAALDVMNKM RALNQFRR

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed: 17372194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BCG / Pasteur 1173P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL72714.1.
RefSeq	YP_978812.1. NC_008769.1.
3D structure databases
ProteinModelPortal	A1KM51.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KM51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000021160; EBMYCP00000020910; EBMYCG00000021155.
GeneID	4698960.
GenomeReviews	Gene locus BCG_2726 in contig AM408590_GR.
KEGG	mbb:BCG_2726.
PATRIC	18016448. VBIMycBov80988_2970.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1249.
GeneTree	EBGT00050000014575.
HOGENOM	HBG515043.
OMA	RSTHFDA.
ProtClustDB	PRK05249.
Enzyme and pathway databases
BioCyc	MBOV410289:BCG_2726-MONOMER.
Family and domain databases
HAMAP	MF_00247. SthA. [Tree]
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR022962. STH. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KO	K00322.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

STHA_MYCBP

Accession

Primary (citable) accession number: A1KM51

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program