A1KLS8 (SYH_MYCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidine--tRNA ligase
EC=6.1.1.21

Alternative name(s):
Histidyl-tRNA synthetase
Short name=HisRS

Gene names

Name:	hisS
Ordered Locus Names:	BCG_2603c

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis › Mycobacterium bovis BCG

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP-Rule MF_00127
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP histidine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Histidine--tRNA ligase HAMAP-Rule MF_00127		PRO_1000016396

Sequences

	Sequence		Length	Mass (Da)
	A1KLS8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C85FBA7B1C7D607F	FASTA	423	45,149
	10 20 30 40 50 60 MTEFSSFSAP KGVPDYVPPD SAQFVAVRDG LLAAARQAGY SHIELPIFED TALFARGVGE 70 80 90 100 110 120 STDVVSKEMY TFADRGDRSV TLRPEGTAGV VRAVIEHGLD RGALPVKLCY AGPFFRYERP 130 140 150 160 170 180 QAGRYRQLQQ VGVEAIGVDD PALDAEVIAI ADAGFRSLGL DGFRLEITSL GDESCRPQYR 190 200 210 220 230 240 ELLQEFLFGL DLDEDTRRRA GINPLRVLDD KRPELRAMTA SAPVLLDHLS DVAKQHFDTV 250 260 270 280 290 300 LAHLDALGVP YVINPRMVRG LDYYTKTAFE FVHDGLGAQS GIGGGGRYDG LMHQLGGQDL 310 320 330 340 350 360 SGIGFGLGVD RTVLALRAEG KTAGDSARCD VFGVPLGEAA KLRLAVLAGR LRAAGVRVDL 370 380 390 400 410 420 AYGDRGLKGA MRAAARSGAR VALVAGDRDI EAGTVAVKDL TTGEQVSVSM DSVVAEVISR LAG

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BCG / Pasteur 1173P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL72591.1.
RefSeq	YP_978689.1. NC_008769.1.
3D structure databases
ProteinModelPortal	A1KLS8.
SMR	A1KLS8. Positions 31-422.
ModBase	Search...
Protein-protein interaction databases
STRING	410289.BCG_2603c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL72591; CAL72591; BCG_2603c.
GeneID	4696840.
KEGG	mbb:BCG_2603c.
PATRIC	18016190. VBIMycBov80988_2845.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0124.
HOGENOM	HOG000018072.
KO	K01892.
OMA	CGGGNFK.
ProtClustDB	PRK00037.
Enzyme and pathway databases
BioCyc	MBOV410289:GJW7-2639-MONOMER.
Family and domain databases
Gene3D	3.40.50.800. 1 hit.
HAMAP	MF_00127. His_tRNA_synth.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR015807. His-tRNA-ligase. IPR004516. HisRS/HisZ. [Graphical view]
PANTHER	PTHR11476. PTHR11476. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. [Graphical view]
PIRSF	PIRSF001549. His-tRNA_synth. 1 hit.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00442. hisS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYH_MYCBP

Accession

Primary (citable) accession number: A1KLS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program