A1KLN4 (AROQ_MYCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type II DHQase

Gene names

Name:	aroQ
Ordered Locus Names:	BCG_2559c

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis › Mycobacterium bovis BCG

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a trans-dehydration via an enolate intermediate By similarity. HAMAP-Rule MF_00169
Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP-Rule MF_00169
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP-Rule MF_00169
Subunit structure	Homododecamer By similarity.
Sequence similarities	Belongs to the type-II 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	3-dehydroquinate dehydratase HAMAP-Rule MF_00169		PRO_1000023487
Regions
	Region	103 – 104	2	Substrate binding By similarity
Sites
	Active site	25	1	Proton acceptor By similarity
	Active site	102	1	Proton donor By similarity
	Binding site	76	1	Substrate By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	113	1	Substrate By similarity
	Site	20	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KLN4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 9EE0E1B14B4DB5F6	FASTA	147	15,790
	10 20 30 40 50 60 MSELIVNVIN GPNLGRLGRR EPAVYGGTTH DELVALIERE AAELGLKAVV RQSDSEAQLL 70 80 90 100 110 120 DWIHQAADAA EPVILNAGGL THTSVALRDA CAELSAPLIE VHISNVHARE EFRRHSYLSP 130 140 IATGVIVGLG IQGYLLALRY LAEHVGT

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BCG / Pasteur 1173P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL72547.1.
RefSeq	YP_978645.1. NC_008769.1.
3D structure databases
ProteinModelPortal	A1KLN4.
SMR	A1KLN4. Positions 1-144.
ModBase	Search...
Protein-protein interaction databases
STRING	410289.BCG_2559c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL72547; CAL72547; BCG_2559c.
GeneID	4699191.
KEGG	mbb:BCG_2559c.
PATRIC	18016095. VBIMycBov80988_2798.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0757.
HOGENOM	HOG000217278.
KO	K03786.
OMA	EPDVYGA.
ProtClustDB	PRK05395.
Enzyme and pathway databases
BioCyc	MBOV410289:GJW7-2595-MONOMER.
UniPathway	UPA00053; UER00086.
Family and domain databases
Gene3D	3.40.50.9100. 1 hit.
HAMAP	MF_00169. AroQ.
InterPro	IPR001874. DHquinase_II. IPR018509. DHquinase_II_CS. [Graphical view]
PANTHER	PTHR21272. PTHR21272. 1 hit.
Pfam	PF01220. DHquinase_II. 1 hit. [Graphical view]
PIRSF	PIRSF001399. DHquinase_II. 1 hit.
ProDom	PD004527. DHquinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF52304. DHquinase_II. 1 hit.
TIGRFAMs	TIGR01088. aroQ. 1 hit.
PROSITE	PS01029. DEHYDROQUINASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	A1KLN4.

Entry information

Entry name

AROQ_MYCBP

Accession

Primary (citable) accession number: A1KLN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program