Reviewed, UniProtKB/Swiss-Prot A1KLH6 (PLSB_MYCBP)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	BCG_2500c

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	789 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 789	789	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049438
Regions
	Motif	275 – 280	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A1KLH6-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: B78D7D9A9796EA22	FASTA	789	88,368
	10 20 30 40 50 60 MTKPAADASA VLTAEDTLVL ASTATPVEME LIMGWLGQQR ARHPDSKFDI LKLPPRNAPP 70 80 90 100 110 120 AALTALVEQL EPGFASSPQS GEDRSIVPVR VIWLPPADRS RAGKVAALLP GRDPYHPSQR 130 140 150 160 170 180 QQRRILRTDP RRARVVAGES AKVSELRQQW RDTTVAEHKR DFAQFVSRRA LLALARAEYR 190 200 210 220 230 240 ILGPQYKSPR LVKPEMLASA RFRAGLDRIP GATVEDAGKM LDELSTGWSQ VSVDLVSVLG 250 260 270 280 290 300 RLASRGFDPE FDYDEYQVAA MRAALEAHPA VLLFSHRSYI DGVVVPVAMQ DNRLPPVHMF 310 320 330 340 350 360 GGINLSFGLM GPLMRRSGMI FIRRNIGNDP LYKYVLKEYV GYVVEKRFNL SWSIEGTRSR 370 380 390 400 410 420 TGKMLPPKLG LMSYVADAYL DGRSDDILLQ GVSICFDQLH EITEYAAYAR GAEKTPEGLR 430 440 450 460 470 480 WLYNFIKAQG ERNFGKIYVR FPEAVSMRQY LGAPHGELTQ DPAAKRLALQ KMSFEVAWRI 490 500 510 520 530 540 LQATPVTATG LVSALLLTTR GTALTLDQLH HTLQDSLDYL ERKQSPVSTS ALRLRSREGV 550 560 570 580 590 600 RAAADALSNG HPVTRVDSGR EPVWYIAPDD EHAAAFYRNS VIHAFLETSI VELALAHAKH 610 620 630 640 650 660 AEGDRVAAFW AQAMRLRDLL KFDFYFADST AFRANIAQEM AWHQDWEDHL GVGGNEIDAM 670 680 690 700 710 720 LYAKRPLMSD AMLRVFFEAY EIVADVLRDA PPDIGPEELT ELALGLGRQF VAQGRVRSSE 730 740 750 760 770 780 PVSTLLFATA RQVAVDQELI APAADLAERR VAFRRELRNI LRDFDYVEQI ARNQFVAREF KARQGRDRI

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed: 17372194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM408590 Genomic DNA. Translation: CAL72488.1.
RefSeq	YP_978587.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1KLH6.
Genome annotation databases
GeneID	4695710.
GenomeReviews	Gene locus BCG_2500c in contig AM408590_GR.
KEGG	mbb:BCG_2500c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QGERNYG.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_MYCBP

Accession

Primary (citable) accession number: A1KLH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)