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Protein

Proteasome-associated ATPase

Gene

mpa

Organism
Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATPase which is responsible for recognizing, binding, unfolding and translocation of pupylated proteins into the bacterial 20S proteasome core particle. May be essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase may function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis.UniRule annotation

Pathwayi: proteasomal Pup-dependent pathway

This protein is involved in the pathway proteasomal Pup-dependent pathway, which is part of Protein degradation.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway proteasomal Pup-dependent pathway and in Protein degradation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi296 – 3016ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBOV410289:GJW7-2158-MONOMER.
UniPathwayiUPA00997.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome-associated ATPaseUniRule annotation
Alternative name(s):
AAA ATPase forming ring-shaped complexesUniRule annotation
Short name:
ARCUniRule annotation
Mycobacterial proteasome ATPaseUniRule annotation
Gene namesi
Name:mpaUniRule annotation
Ordered Locus Names:BCG_2132c
OrganismiMycobacterium bovis (strain BCG / Pasteur 1173P2)
Taxonomic identifieri410289 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001472 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 609609Proteasome-associated ATPasePRO_0000396994Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Assembles into a hexameric ring structure that caps the 20S proteasome core. Strongly interacts with the prokaryotic ubiquitin-like protein Pup through a hydrophobic interface; the interacting region of ARC lies in its N-terminal coiled-coil domain. There is one Pup binding site per ARC hexamer ring. Upon ATP-binding, the C-terminus of ARC interacts with the alpha-rings of the proteasome core, possibly by binding to the intersubunit pockets.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA1KKF8.
SMRiA1KKF8. Positions 52-605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni608 – 6092Docks into pockets in the proteasome alpha-ringUniRule annotation

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili20 – 9677UniRule annotationAdd
BLAST

Domaini

Consists of three main regions, an N-terminal coiled-coil domain that binds to protein Pup and functions as a docking station, an interdomain involved in ARC hexamerization, and a C-terminal ATPase domain of the AAA type.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000245286.
KOiK13527.
OMAiCVDEFKE.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02112. ARC_ATPase. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR032501. Prot_ATP_ID_OB.
IPR022482. Proteasome_ATPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16450. Prot_ATP_ID_OB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03689. pup_AAA. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1KKF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGESERSEAF GIPRDSPLSS GDAAELEQLR REAAVLREQL ENAVGSHAPT
60 70 80 90 100
RSARDIHQLE ARIDSLAARN SKLMETLKEA RQQLLALREE VDRLGQPPSG
110 120 130 140 150
YGVLLATHDD DTVDVFTSGR KMRLTCSPNI DAASLKKGQT VRLNEALTVV
160 170 180 190 200
EAGTFEAVGE ISTLREILAD GHRALVVGHA DEERVVWLAD PLIAEDLPDG
210 220 230 240 250
LPEALNDDTR PRKLRPGDSL LVDTKAGYAF ERIPKAEVED LVLEEVPDVS
260 270 280 290 300
YADIGGLSRQ IEQIRDAVEL PFLHKELYRE YSLRPPKGVL LYGPPGCGKT
310 320 330 340 350
LIAKAVANSL AKKMAEVRGD DAHEAKSYFL NIKGPELLNK FVGETERHIR
360 370 380 390 400
LIFQRAREKA SEGTPVIVFF DEMDSIFRTR GTGVSSDVET TVVPQLLSEI
410 420 430 440 450
DGVEGLENVI VIGASNREDM IDPAILRPGR LDVKIKIERP DAEAAQDIYS
460 470 480 490 500
KYLTEFLPVH ADDLAEFDGD RSACIKAMIE KVVDRMYAEI DDNRFLEVTY
510 520 530 540 550
ANGDKEVMYF KDFNSGAMIQ NVVDRAKKNA IKSVLETGQP GLRIQHLLDS
560 570 580 590 600
IVDEFAENED LPNTTNPDDW ARISGKKGER IVYIRTLVTG KSSSASRAID

TESNLGQYL
Length:609
Mass (Da):67,401
Last modified:February 6, 2007 - v1
Checksum:i4D5F4E630614C58D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM408590 Genomic DNA. Translation: CAL72120.1.
RefSeqiWP_003411035.1. NC_008769.1.

Genome annotation databases

EnsemblBacteriaiCAL72120; CAL72120; BCG_2132c.
KEGGimbb:BCG_2132c.
PATRICi18015142. VBIMycBov80988_2332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM408590 Genomic DNA. Translation: CAL72120.1.
RefSeqiWP_003411035.1. NC_008769.1.

3D structure databases

ProteinModelPortaliA1KKF8.
SMRiA1KKF8. Positions 52-605.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL72120; CAL72120; BCG_2132c.
KEGGimbb:BCG_2132c.
PATRICi18015142. VBIMycBov80988_2332.

Phylogenomic databases

HOGENOMiHOG000245286.
KOiK13527.
OMAiCVDEFKE.

Enzyme and pathway databases

UniPathwayiUPA00997.
BioCyciMBOV410289:GJW7-2158-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02112. ARC_ATPase. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR032501. Prot_ATP_ID_OB.
IPR022482. Proteasome_ATPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16450. Prot_ATP_ID_OB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03689. pup_AAA. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARC_MYCBP
AccessioniPrimary (citable) accession number: A1KKF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: February 6, 2007
Last modified: September 7, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.