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Protein

Diacylglycerol acyltransferase/mycolyltransferase Ag85B

Gene

fbpB

Organism
Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).By similarity

Catalytic activityi

2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate.
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661NucleophileBy similarity
Binding sitei166 – 1661SubstrateBy similarity
Binding sitei194 – 1941SubstrateBy similarity
Active sitei270 – 2701By similarity
Binding sitei279 – 2791SubstrateBy similarity
Active sitei302 – 3021By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciMBOV410289:GJW7-1949-MONOMER.

Protein family/group databases

ESTHERimyctu-a85b. A85-Mycolyl-transferase.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol acyltransferase/mycolyltransferase Ag85B (EC:2.3.1.122, EC:2.3.1.20)
Short name:
DGAT
Alternative name(s):
30 kDa extracellular protein
Acyl-CoA:diacylglycerol acyltransferase
Antigen 85 complex B
Short name:
85B
Short name:
Ag85B
Extracellular alpha-antigen
Fibronectin-binding protein B
Short name:
Fbps B
Gene namesi
Name:fbpB
Ordered Locus Names:BCG_1923c
OrganismiMycobacterium bovis (strain BCG / Pasteur 1173P2)
Taxonomic identifieri410289 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001472 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040By similarityAdd
BLAST
Chaini41 – 325285Diacylglycerol acyltransferase/mycolyltransferase Ag85BPRO_0000285178Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi127 ↔ 132By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliA1KJU9.
SMRiA1KJU9. Positions 42-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate bindingBy similarity
Regioni98 – 10811Fibronectin-bindingAdd
BLAST
Regioni272 – 2754Substrate bindingBy similarity
Regioni302 – 3043Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the mycobacterial A85 antigen family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000049505.
KOiK18851.
OMAiKPACGKS.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1KJU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVSRKIRA WGRRLMIGTA AAVVLPGLVG LAGGAATAGA FSRPGLPVEY
60 70 80 90 100
LQVPSPSMGR DIKVQFQSGG NNSPAVYLLD GLRAQDDYNG WDINTPAFEW
110 120 130 140 150
YYQSGLSIVM PVGGQSSFYS DWYSPACGKA GCQTYKWETL LTSELPQWLS
160 170 180 190 200
ANRAVKPTGS AAIGLSMAGS SAMILAAYHP QQFIYAGSLS ALLDPSQGMG
210 220 230 240 250
PSLIGLAMGD AGGYKAADMW GPSSDPAWER NDPTQQIPKL VANNTRLWVY
260 270 280 290 300
CGNGTPNELG GANIPAEFLE NFVRSSNLKF QDAYNAAGGH NAVFNFPPNG
310 320
THSWEYWGAQ LNAMKGDLQS SLGAG
Length:325
Mass (Da):34,547
Last modified:February 6, 2007 - v1
Checksum:iB7775F40C131BC7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62397 Genomic DNA. Translation: CAA44268.1.
AM408590 Genomic DNA. Translation: CAL71910.1.
PIRiS29663.
RefSeqiWP_011799226.1. NC_008769.1.

Genome annotation databases

EnsemblBacteriaiCAL71910; CAL71910; BCG_1923c.
KEGGimbb:BCG_1923c.
PATRICi18014674. VBIMycBov80988_2097.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62397 Genomic DNA. Translation: CAA44268.1.
AM408590 Genomic DNA. Translation: CAL71910.1.
PIRiS29663.
RefSeqiWP_011799226.1. NC_008769.1.

3D structure databases

ProteinModelPortaliA1KJU9.
SMRiA1KJU9. Positions 42-325.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERimyctu-a85b. A85-Mycolyl-transferase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL71910; CAL71910; BCG_1923c.
KEGGimbb:BCG_1923c.
PATRICi18014674. VBIMycBov80988_2097.

Phylogenomic databases

HOGENOMiHOG000049505.
KOiK18851.
OMAiKPACGKS.

Enzyme and pathway databases

BioCyciMBOV410289:GJW7-1949-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA85B_MYCBP
AccessioniPrimary (citable) accession number: A1KJU9
Secondary accession number(s): P12942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 6, 2007
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.