A1KJ66 (SYFA_MYCBP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BCG_1688

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000006861
Sites
	Metal binding	259	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KJ66 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C4C46AA8CD99349B	FASTA	341	37,415
	10 20 30 40 50 60 MLSPEALTTA VDAAQQAIAL ADTLDVLARV KTEHLGDRSP LALARQALAV LPKEQRAEAG 70 80 90 100 110 120 KRVNAARNAA QRSYDERLAT LRAERDAAVL VAEGIDVTLP STRVPAGARH PIIMLAEHVA 130 140 150 160 170 180 DTFIAMGWEL AEGPEVETEQ FNFDALNFPA DHPARGEQDT FYIAPEDSRQ LLRTHTSPVQ 190 200 210 220 230 240 IRTLLARELP VYIISIGRTF RTDELDATHT PIFHQVEGLA VDRGLSMAHL RGTLDAFARA 250 260 270 280 290 300 EFGPSARTRI RPHFFPFTEP SAEVDVWFAN KIGGADWVEW GGCGMVHPNV LRATGIDPDL 310 320 330 340 YSGFAFGMGL ERTLQFRNGI PDMRDMVEGD VRFSLPFGVG A

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed: 17372194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BCG / Pasteur 1173P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL71675.1.
RefSeq	YP_977780.1. NC_008769.1.
3D structure databases
ProteinModelPortal	A1KJ66.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KJ66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000021497; EBMYCP00000021247; EBMYCG00000021492.
GeneID	4695647.
GenomeReviews	Gene locus BCG_1688 in contig AM408590_GR.
KEGG	mbb:BCG_1688.
PATRIC	18014164. VBIMycBov80988_1843.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
GeneTree	EBGT00050000016426.
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	MBOV410289:BCG_1688-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_MYCBP

Accession

Primary (citable) accession number: A1KJ66

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program