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A1KJ05 (BIOB_MYCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:BCG_1627
OrganismMycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]
Taxonomic identifier410289 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Biotin synthase HAMAP-Rule MF_01694
PRO_0000381477

Sites

Metal binding851Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding891Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding921Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1281Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1611Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2201Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2901Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A1KJ05 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: F3B0A5821B3F14A2

FASTA34937,550
        10         20         30         40         50         60 
MTQAATRPTN DAGQDGGNNS DILVVARQQV LQRGEGLNQD QVLAVLQLPD DRLEELLALA 

        70         80         90        100        110        120 
HEVRMRWCGP EVEVEGIISL KTGGCPEDCH FCSQSGLFAS PVRSAWLDIP SLVEAAKQTA 

       130        140        150        160        170        180 
KSGATEFCIV AAVRGPDERL MAQVAAGIEA IRNEVEINIA CSLGMLTAEQ VDQLAARGVH 

       190        200        210        220        230        240 
RYNHNLETAR SFFANVVTTH TWEERWQTLS MVRDAGMEVC CGGILGMGET LQQRAEFAAE 

       250        260        270        280        290        300 
LAELGPDEVP LNFLNPRPGT PFADLEVMPV GDALKAVAAF RLALPRTMLR FAGGREITLG 

       310        320        330        340 
DLGAKRGILG GINAVIVGNY LTTLGRPAEA DLELLDELQM PLKALNASL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM408590 Genomic DNA. Translation: CAL71614.1.
RefSeqYP_977719.1. NC_008769.1.

3D structure databases

ProteinModelPortalA1KJ05.
SMRA1KJ05. Positions 45-340.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING410289.BCG_1627.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL71614; CAL71614; BCG_1627.
GeneID4696627.
KEGGmbb:BCG_1627.
PATRIC18014032. VBIMycBov80988_1778.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMADETQALC.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycMBOV410289:GJW7-1652-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_MYCBP
AccessionPrimary (citable) accession number: A1KJ05
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways