Reviewed, UniProtKB/Swiss-Prot A1KIK4 (RIBBA_MYCBP)

Names and origin

Protein names

Recommended name:
    Riboflavin biosynthesis protein ribBA
Including the following 2 domains:
    1- Recommended name:
            3,4-dihydroxy-2-butanone 4-phosphate synthase
                Short name=DHBP synthase
              EC=4.1.99.12
    2- Recommended name:
            GTP cyclohydrolase-2
              EC=3.5.4.25
        Alternative name(s):
            GTP cyclohydrolase II

Gene names

Name:	ribBA
Ordered Locus Names:	BCG_1476

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_01283 Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity. HAMAP MF_01283
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_01283 GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate. HAMAP MF_01283
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. HAMAP MF_01283 Binds 1 zinc ion per subunit By similarity. HAMAP MF_01283
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_01283 Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP MF_01283
Sequence similarities	In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	GTP-binding Magnesium Manganese Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase II activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Riboflavin biosynthesis protein ribBA HAMAP MF_01283		PRO_1000067424
Regions
	Nucleotide binding	259 – 263	5	GTP By similarity
	Nucleotide binding	303 – 305	3	GTP By similarity
	Region	1 – 204	204	DHBP synthase HAMAP MF_01283
	Region	28 – 29	2	D-ribulose 5-phosphate binding By similarity
	Region	141 – 145	5	D-ribulose 5-phosphate binding By similarity
	Region	205 – 425	221	GTP cyclohydrolase II HAMAP MF_01283
Sites
	Active site	337	1	Proton acceptor; for GTP cyclohydrolase activity Potential
	Active site	339	1	Nucleophile; for GTP cyclohydrolase activity By similarity
	Metal binding	29	1	Magnesium or manganese 1 By similarity
	Metal binding	29	1	Magnesium or manganese 2 By similarity
	Metal binding	144	1	Magnesium or manganese 2 By similarity
	Metal binding	264	1	Zinc; catalytic By similarity
	Metal binding	275	1	Zinc; catalytic By similarity
	Metal binding	277	1	Zinc; catalytic By similarity
	Binding site	33	1	D-ribulose 5-phosphate By similarity
	Binding site	165	1	D-ribulose 5-phosphate By similarity
	Binding site	280	1	GTP By similarity
	Binding site	325	1	GTP By similarity
	Binding site	360	1	GTP By similarity
	Binding site	365	1	GTP By similarity
	Site	127	1	Essential for DHBP synthase activity By similarity
	Site	165	1	Essential for DHBP synthase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KIK4-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: FA7F73868814591F	FASTA	425	46,017
	10 20 30 40 50 60 MTRLDSVERA VADIAAGKAV IVIDDEDREN EGDLIFAAEK ATPEMVAFMV RYTSGYLCVP 70 80 90 100 110 120 LDGAICDRLG LLPMYAVNQD KHGTAYTVTV DARNGIGTGI SASDRATTMR LLADPTSVAD 130 140 150 160 170 180 DFTRPGHVVP LRAKDGGVLR RPGHTEAAVD LARMAGLQPA GAICEIVSQK DEGSMAHTDE 190 200 210 220 230 240 LRVFADEHGL ALITIADLIE WRRKHEKHIE RVAEARIPTR HGEFRAIGYT SIYEDVEHVA 250 260 270 280 290 300 LVRGEIAGPN ADGDDVLVRV HSECLTGDVF GSRRCDCGPQ LDAALAMVAR EGRGVVLYMR 310 320 330 340 350 360 GHEGRGIGLM HKLQAYQLQD AGADTVDANL KLGLPADARD YGIGAQILVD LGVRSMRLLT 370 380 390 400 410 420 NNPAKRVGLD GYGLHIIERV PLPVRANAEN IRYLMTKRDK LGHDLAGLDD FHESVHLPGE FGGAL

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed: 17372194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL71463.1.
RefSeq	YP_977568.1.
3D structure databases
SMR	A1KIK4. Positions 3-204, 208-384.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KIK4.
Genome annotation databases
GeneID	4696694.
GenomeReviews	Gene locus BCG_1476 in contig AM408590_GR.
KEGG	mbb:BCG_1476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0807.
HOGENOM	HBG735778.
OMA	LRCDCRM.
Family and domain databases
HAMAP	MF_01283. RibBA. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. IPR000926. GTP_CycHdrlase_II. IPR016299. Riboflavin_synth_RibA. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
Pfam	PF00926. DHBP_synthase. 1 hit. PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
PIRSF	PIRSF001259. RibA. 1 hit.
TIGRFAMs	TIGR00505. ribA. 1 hit. TIGR00506. ribB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBBA_MYCBP

Accession

Primary (citable) accession number: A1KIK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)