A1KGU1 (A1KGU1_MYCBP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485

Gene names

Name:	purF EMBL CAL70846.1
Ordered Locus Names:	BCG_0860

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3 Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Ontologies

Keywords
Biological process	Purine biosynthesis PIRNR PIRNR000485
Ligand	Iron Iron-sulfur PIRSR PIRSR000485-3 Magnesium PIRNR PIRNR000485 Metal-binding
Molecular function	Glycosyltransferase PIRNR PIRNR000485 EMBL CAL70846.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	35	1	For GATase activity By similarity PIRSR PIRSR000485-1
	Metal binding	276	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	422	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	478	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	481	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

	Sequence		Length	Mass (Da)
	A1KGU1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: A2FE9D1DD08FA572	FASTA	527	56,162
	10 20 30 40 50 60 MAVDSDYVTD RAAGSRQTVT GQQPEQDLNS PREECGVFGV WAPGEDVAKL TYYGLYALQH 70 80 90 100 110 120 RGQEAAGIAV ADGSQVLVFK DLGLVSQVFD EQTLAAMQGH VAIGHCRYST TGDTTWENAQ 130 140 150 160 170 180 PVFRNTAAGT GVALGHNGNL VNAAALAARA RDAGLIATRC PAPATTDSDI LGALLAHGAA 190 200 210 220 230 240 DSTLEQAALD LLPTVRGAFC LTFMDENTLY ACRDPYGVRP LSLGRLDRGW VVASETAALD 250 260 270 280 290 300 IVGASFVRDI EPGELLAIDA DGVRSTRFAN PTPKGCVFEY VYLARPDSTI AGRSVHAARV 310 320 330 340 350 360 EIGRRLAREC PVEADLVIGV PESGTPAAVG YAQESGVPYG QGLMKNAYVG RTFIQPSQTI 370 380 390 400 410 420 RQLGIRLKLN PLKEVIRGKR LIVVDDSIVR GNTQRALVRM LREAGAVELH VRIASPPVKW 430 440 450 460 470 480 PCFYGIDFPS PAELIANAVE NEDEMLEAVR HAIGADTLGY ISLRGMVAAS EQPTSRLCTA 490 500 510 520 CFDGKYPIEL PRETALGKNV IEHMLANAAR GAALGELAAD DEVPVGR

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed: 17372194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL70846.1.
RefSeq	YP_976955.1. NC_008769.1.
3D structure databases
ProteinModelPortal	A1KGU1.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KGU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000019906; EBMYCP00000019656; EBMYCG00000019901.
GeneID	4695908.
GenomeReviews	Gene locus BCG_0860 in contig AM408590_GR.
KEGG	mbb:BCG_0860.
PATRIC	18012318. VBIMycBov80988_0934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0034.
GeneTree	EBGT00050000016850.
HOGENOM	HBG392416.
OMA	VWAPGEE.
ProtClustDB	PRK07847.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
KO	K00764.
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. PurF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1KGU1_MYCBP

Accession

Primary (citable) accession number: A1KGU1

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)