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A1KG32

- MEND_MYCBP

UniProt

A1KG32 - MEND_MYCBP

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciMBOV410289:GJW7-606-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:BCG_0600
OrganismiMycobacterium bovis (strain BCG / Pasteur 1173P2)
Taxonomic identifieri410289 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001472: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5545542-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_0000341776Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi410289.BCG_0600.

Structurei

3D structure databases

ProteinModelPortaliA1KG32.
SMRiA1KG32. Positions 1-547.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OMAiWRSAVCR.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

A1KG32-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPSTTQARV VVDELIRGGV RDVVLCPGSR NAPLAFALQD ADRSGRIRLH
60 70 80 90 100
VRIDERTAGY LAIGLAIGAG APVCVAMTSG TAVANLGPAV VEANYARVPL
110 120 130 140 150
IVLSANRPYE LLGTGANQTM EQLGYFGTQV RASISLGLAE DAPERTSALN
160 170 180 190 200
ATWRSATCRV LAAATGARTA NAGPVHFDIP LREPLVPDPE PLGAVTPPGR
210 220 230 240 250
PAGKPWTYTP PVTFDQPLDI DLSVDTVVIS GHGAGVHPNL AALPTVAEPT
260 270 280 290 300
APRSGDNPLH PLALPLLRPQ QVIMLGRPTL HRPVSVLLAD AEVPVFALTT
310 320 330 340 350
GPRWPDVSGN SQATGTRAVT TGAPRPAWLD RCAAMNRHAI AAVREQLAAH
360 370 380 390 400
PLTTGLHVAA AVSHALRPGD QLVLGASNPV RDVALAGLDT RGIRVRSNRG
410 420 430 440 450
VAGIDGTVST AIGAALAYEG AHERTGSPDS PPRTIALIGD LTFVHDSSGL
460 470 480 490 500
LIGPTEPIPR SLTIVVSNDN GGGIFELLEQ GDPRFSDVSS RIFGTPHDVD
510 520 530 540 550
VGALCRAYHV ESRQIEVDEL GPTLDQPGAG MRVLEVKADR SSLRQLHAAI

KAAL
Length:554
Mass (Da):57,836
Last modified:February 6, 2007 - v1
Checksum:i879423AAB00F36A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM408590 Genomic DNA. Translation: CAL70585.1.
RefSeqiYP_976696.1. NC_008769.1.

Genome annotation databases

EnsemblBacteriaiCAL70585; CAL70585; BCG_0600.
GeneIDi4697151.
KEGGimbb:BCG_0600.
PATRICi18011735. VBIMycBov80988_0647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM408590 Genomic DNA. Translation: CAL70585.1 .
RefSeqi YP_976696.1. NC_008769.1.

3D structure databases

ProteinModelPortali A1KG32.
SMRi A1KG32. Positions 1-547.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 410289.BCG_0600.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL70585 ; CAL70585 ; BCG_0600 .
GeneIDi 4697151.
KEGGi mbb:BCG_0600.
PATRICi 18011735. VBIMycBov80988_0647.

Phylogenomic databases

eggNOGi COG1165.
HOGENOMi HOG000218359.
KOi K02551.
OMAi WRSAVCR.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 .
UPA01057 ; UER00164 .
BioCyci MBOV410289:GJW7-606-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view ]
Pfami PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BCG / Pasteur 1173P2.

Entry informationi

Entry nameiMEND_MYCBP
AccessioniPrimary (citable) accession number: A1KG32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: February 6, 2007
Last modified: November 26, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3