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A1KF31 (PCKG_MYCBP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:pckG
Ordered Locus Names:BCG_0248
OrganismMycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]
Taxonomic identifier410289 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex ›

Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity.

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: HAMAP

manganese ion binding

Inferred from electronic annotation. Source: HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 606606Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000060291

Regions

Nucleotide binding272 – 2776GTP By similarity
Nucleotide binding515 – 5184GTP By similarity
Region387 – 3893Substrate binding By similarity

Sites

Active site2731 By similarity
Metal binding2291Manganese By similarity
Metal binding2491Manganese By similarity
Metal binding2961Manganese By similarity
Binding site811Substrate By similarity
Binding site2221Substrate; via amide nitrogen By similarity
Binding site2291Substrate By similarity
Binding site2711Substrate By similarity
Binding site3891GTP By similarity
Binding site4201GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1KF31 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: AEE29412E6BCCAE3

FASTA60667,253
        10         20         30         40         50         60 
MTSATIPGLD TAPTNHQGLL SWVEEVAELT QPDRVVFTDG SEEEFQRLCD QLVEAGTFIR 

        70         80         90        100        110        120 
LNPEKHKNSY LALSDPSDVA RVESRTYICS AKEIDAGPTN NWMDPGEMRS IMKDLYRGCM 

       130        140        150        160        170        180 
RGRTMYVVPF CMGPLGAEDP KLGVEITDSE YVVVSMRTMT RMGKAALEKM GDDGFFVKAL 

       190        200        210        220        230        240 
HSVGAPLEPG QKDVAWPCSE TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA 

       250        260        270        280        290        300 
HDEGWLAEHM LILKLISPEN KAYYFAAAFP SACGKTNLAM LQPTIPGWRA ETLGDDIAWM 

       310        320        330        340        350        360 
RFGKDGRLYA VNPEFGFFGV APGTNWKSNP NAMRTIAAGN TVFTNVALTD DGDVWWEGLE 

       370        380        390        400        410        420 
GDPQHLIDWK GNDWYFRETE TNAAHPNSRY CTPMSQCPIL APEWDDPQGV PISGILFGGR 

       430        440        450        460        470        480 
RKTTVPLVTE ARDWQHGVFI GATLGSEQTA AAEGKVGNVR RDPMAMLPFL GYNVGDYFQH 

       490        500        510        520        530        540 
WINLGKHADE SKLPKVFFVN WFRRGDDGRF LWPGFGENSR VLKWIVDRIE HKAGGATTPI 

       550        560        570        580        590        600 
GTVPAVEDLD LDGLDVDAAD VAAALAVDAD EWRQELPLIE EWLQFVGEKL PTGVKDEFDA 


LKERLG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM408590 Genomic DNA. Translation: CAL70232.1.
RefSeqYP_976346.1. NC_008769.1.

3D structure databases

ProteinModelPortalA1KF31.
SMRA1KF31. Positions 12-605.
ModBaseSearch...

Protein-protein interaction databases

STRING410289.BCG_0248.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL70232; CAL70232; BCG_0248.
GeneID4697998.
KEGGmbb:BCG_0248.
PATRIC18010984. VBIMycBov80988_0272.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAWMRFGED.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycMBOV410289:GJW7-252-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_MYCBP
AccessionPrimary (citable) accession number: A1KF31
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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