A1KF09 (A1KF09_MYCBP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase HAMAP MF_00012
Short name=DAD HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD HAMAP MF_00012 EMBL CAL70210.1
Ordered Locus Names:	BCG_0226c

Organism

Mycobacterium bovis (strain BCG / Pasteur 1173P2) [Complete proteome] [HAMAP]

Taxonomic identifier

410289 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL CAL70210.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	137	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	214	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	A1KF09 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 6ECC279BF886A0F1	FASTA	575	59,352
	10 20 30 40 50 60 MPQTTDEAAS VSTVADIKPR SRDVTDGLEK AAARGMLRAV GMDDEDFAKP QIGVASSWNE 70 80 90 100 110 120 ITPCNLSLDR LANAVKEGVF SAGGYPLEFG TISVSDGISM GHEGMHFSLV SREVIADSVE 130 140 150 160 170 180 VVMQAERLDG SVLLAGCDKS LPGMLMAAAR LDLAAVFLYA GSILPGRAKL SDGSERDVTI 190 200 210 220 230 240 IDAFEAVGAC SRGLMSRADV DAIERAICPG EGACGGMYTA NTMASAAEAL GMSLPGSAAP 250 260 270 280 290 300 PATDRRRDGF ARRSGQAVVE LLRRGITARD ILTKEAFENA IAVVMAFGGS TNAVLHLLAI 310 320 330 340 350 360 AHEANVALSL QDFSRIGSGV PHLADVKPFG RHVMSDVDHI GGVPVVMKAL LDAGLLHGDC 370 380 390 400 410 420 LTVTGHTMAE NLAAITPPDP DGKVLRALAN PIHPSGGITI LHGSLAPEGA VVKTAGFDSD 430 440 450 460 470 480 VFEGTARVFD GERAALDALE DGTITVGDAV VIRYEGPKGG PGMREMLAIT GAIKGAGLGK 490 500 510 520 530 540 DVLLLTDGRF SGGTTGLCVG HIAPEAVDGG PIALLRNGDR IRLDVAGRVL DVLADPAEFA 550 560 570 SRQQDFSPPP PRYTTGVLSK YVKLVSSAAV GAVCG

References

[1]

"Genome plasticity of BCG and impact on vaccine efficacy." Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P., Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K., Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C., Barrell B.G., Parkhill J., Cole S.T. Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007) [PubMed: 17372194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM408590 Genomic DNA. Translation: CAL70210.1.
RefSeq	YP_976324.1. NC_008769.1.
3D structure databases
ProteinModelPortal	A1KF09.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KF09.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000020986; EBMYCP00000020736; EBMYCG00000020981.
GeneID	4697026.
GenomeReviews	Gene locus BCG_0226c in contig AM408590_GR.
KEGG	mbb:BCG_0226c.
PATRIC	18010938. VBIMycBov80988_0249.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
GeneTree	EBGT00050000016366.
HOGENOM	HBG671001.
OMA	NMPGAMI.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1KF09_MYCBP

Accession

Primary (citable) accession number: A1KF09

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)