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A1KCM8

- TRMB_AZOSB

UniProt

A1KCM8 - TRMB_AZOSB

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, azo3968
Organism
Azoarcus sp. (strain BH72)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831S-adenosyl-L-methionine By similarity
Binding sitei108 – 1081S-adenosyl-L-methionine By similarity
Binding sitei135 – 1351S-adenosyl-L-methionine By similarity
Active sitei158 – 1581 By similarity
Binding sitei158 – 1581S-adenosyl-L-methionine By similarity
Binding sitei162 – 1621Substrate By similarity
Binding sitei194 – 1941Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:azo3968
    OrganismiAzoarcus sp. (strain BH72)
    Taxonomic identifieri62928 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus
    ProteomesiUP000002588: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 250250tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000288120Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi62928.azo3968.

    Structurei

    3D structure databases

    ProteinModelPortaliA1KCM8.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni229 – 2324Substrate binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiQDNFLCC.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1KCM8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEADNKPVS ATVELIAQDR PEHRPEHRFS SRSIRSFVLR QGRMSGAQQR    50
    YLEDMLPKIG IEYRPAPLDL DDAFGRSAPK ILEIGFGMGE TTAKIAAAHP 100
    EQDYLGIEVH GPGVGAMCKL VAENGLANVR IMQHDAVEVM RDMIADGALA 150
    GVHVFFPDPW RKARHYKRRL IQPDFVALVA QKLAPGGYLH CATDWEHYAH 200
    WMLEILAAEP LLQNTADGFA PRPDYRPLTK FENRGLKLGH GVWDLVFRRR 250
    Length:250
    Mass (Da):28,154
    Last modified:February 6, 2007 - v1
    Checksum:iECD49329C11AFFC9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM406670 Genomic DNA. Translation: CAL96584.1.
    RefSeqiWP_011767690.1. NC_008702.1.
    YP_935470.1. NC_008702.1.

    Genome annotation databases

    EnsemblBacteriaiCAL96584; CAL96584; azo3968.
    GeneIDi4608217.
    KEGGiazo:azo3968.
    PATRICi21016380. VBIAzoSp26047_4007.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM406670 Genomic DNA. Translation: CAL96584.1 .
    RefSeqi WP_011767690.1. NC_008702.1.
    YP_935470.1. NC_008702.1.

    3D structure databases

    ProteinModelPortali A1KCM8.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 62928.azo3968.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL96584 ; CAL96584 ; azo3968 .
    GeneIDi 4608217.
    KEGGi azo:azo3968.
    PATRICi 21016380. VBIAzoSp26047_4007.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi QDNFLCC.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BH72.

    Entry informationi

    Entry nameiTRMB_AZOSB
    AccessioniPrimary (citable) accession number: A1KCM8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: February 6, 2007
    Last modified: September 3, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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