Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei83S-adenosyl-L-methionineUniRule annotation1
Binding sitei108S-adenosyl-L-methionineUniRule annotation1
Binding sitei135S-adenosyl-L-methionineUniRule annotation1
Active sitei158By similarity1
Binding sitei158S-adenosyl-L-methionineUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Binding sitei194SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciASP62928:G1GJH-4001-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:azo3968
OrganismiAzoarcus sp. (strain BH72)
Taxonomic identifieri62928 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesZoogloeaceaeAzoarcus
Proteomesi
  • UP000002588 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881201 – 250tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST250

Interactioni

Protein-protein interaction databases

STRINGi62928.azo3968

Structurei

3D structure databases

ProteinModelPortaliA1KCM8
SMRiA1KCM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni229 – 232Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

A1KCM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEADNKPVS ATVELIAQDR PEHRPEHRFS SRSIRSFVLR QGRMSGAQQR
60 70 80 90 100
YLEDMLPKIG IEYRPAPLDL DDAFGRSAPK ILEIGFGMGE TTAKIAAAHP
110 120 130 140 150
EQDYLGIEVH GPGVGAMCKL VAENGLANVR IMQHDAVEVM RDMIADGALA
160 170 180 190 200
GVHVFFPDPW RKARHYKRRL IQPDFVALVA QKLAPGGYLH CATDWEHYAH
210 220 230 240 250
WMLEILAAEP LLQNTADGFA PRPDYRPLTK FENRGLKLGH GVWDLVFRRR
Length:250
Mass (Da):28,154
Last modified:February 6, 2007 - v1
Checksum:iECD49329C11AFFC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA Translation: CAL96584.1
RefSeqiWP_011767690.1, NC_008702.1

Genome annotation databases

EnsemblBacteriaiCAL96584; CAL96584; azo3968
KEGGiazo:azo3968

Similar proteinsi

Entry informationi

Entry nameiTRMB_AZOSB
AccessioniPrimary (citable) accession number: A1KCM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: February 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health