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A1KCM8 (TRMB_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:azo3968
OrganismAzoarcus sp. (strain BH72) [Complete proteome] [HAMAP]
Taxonomic identifier62928 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288120

Regions

Region229 – 2324Substrate binding By similarity

Sites

Active site1581 By similarity
Binding site831S-adenosyl-L-methionine By similarity
Binding site1081S-adenosyl-L-methionine By similarity
Binding site1351S-adenosyl-L-methionine By similarity
Binding site1581S-adenosyl-L-methionine By similarity
Binding site1621Substrate By similarity
Binding site1941Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A1KCM8 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: ECD49329C11AFFC9

FASTA25028,154
        10         20         30         40         50         60 
MSEADNKPVS ATVELIAQDR PEHRPEHRFS SRSIRSFVLR QGRMSGAQQR YLEDMLPKIG 

        70         80         90        100        110        120 
IEYRPAPLDL DDAFGRSAPK ILEIGFGMGE TTAKIAAAHP EQDYLGIEVH GPGVGAMCKL 

       130        140        150        160        170        180 
VAENGLANVR IMQHDAVEVM RDMIADGALA GVHVFFPDPW RKARHYKRRL IQPDFVALVA 

       190        200        210        220        230        240 
QKLAPGGYLH CATDWEHYAH WMLEILAAEP LLQNTADGFA PRPDYRPLTK FENRGLKLGH 

       250 
GVWDLVFRRR 

« Hide

References

[1]"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72."
Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. expand/collapse author list , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.
Nat. Biotechnol. 24:1385-1391(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BH72.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM406670 Genomic DNA. Translation: CAL96584.1.
RefSeqYP_935470.1. NC_008702.1.

3D structure databases

ProteinModelPortalA1KCM8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING62928.azo3968.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL96584; CAL96584; azo3968.
GeneID4608217.
KEGGazo:azo3968.
PATRIC21016380. VBIAzoSp26047_4007.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAQDNFLCC.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_AZOSB
AccessionPrimary (citable) accession number: A1KCM8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways