A1KCL7 (DNAE2_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	azo3957

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	1064 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1064	1064	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070588

Sequences

	Sequence		Length	Mass (Da)
	A1KCL7 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 1131DA75605D8C75	FASTA	1,064	116,192
	10 20 30 40 50 60 MATPPYAELH CLSNYSFLRG ASHPEELVAR AWALGYQGLA ITDECSLAGA VRAHTGLAEL 70 80 90 100 110 120 RRDLRAAADA PEASAQDRLL AERAEQFRLV FGAEFRLACG LKLVLLAKNR AGYGNLSALI 130 140 150 160 170 180 TLARQRSEKG SYRLVRADLD AISPAGAVPD CYALWLPDAA SSAEDAHWFA RRFGERGWLA 190 200 210 220 230 240 VELHSGADDA AWLGRTAELA AAAGLPRLAA GDVHMHLRGR RPLQDTLTAI RLGTTVFEAG 250 260 270 280 290 300 TALHPNGERH LRHPLRLARL YPAELLEAAA RLALACDFQL DTLRYEYPQE IVPAGETPAS 310 320 330 340 350 360 YLRRETQAGL ARRYPQGVPP AIADNVEQEL ALIIELGYEP FFLTVYDIVC FARDEGILCQ 370 380 390 400 410 420 GRGSAANSIV CYALGITEVD PARASLLFGR FISRERDEPP DIDVDFEHDR RETVIQYIYG 430 440 450 460 470 480 KYGRDRAALA ATVIRYRTRG ALRDAGRALG FGQPQIDALA RSLAWWDKRD QLPARLIELG 490 500 510 520 530 540 LDPASPRVAK WLDLTGMLIG FPRHLSQHVG GFVISRGPLG RLVPIENAAM PERSVIQWDK 550 560 570 580 590 600 EDLEALGLLK VDVLALGMLS VIRRSLELVG RRRGAPFALP HIPPKDAATF DMLCAADSVG 610 620 630 640 650 660 VFQVESRAQM AMLPRLRPRQ FYDLVVQVAI VRPGPIQGDM VHPYLTNRAD PAASARTLAR 670 680 690 700 710 720 LPADVRAVLE RTLGVPIFQE QVMKLAEVAA GFTPGEADQL RRAMASWRQK GHIERFKQKL 730 740 750 760 770 780 RDGMKARGHD SDFADALCRQ IEGFGEYGFP ESHAASFALL AYASAWLKRH EPEAFLCGLL 790 800 810 820 830 840 NSQPMGFYAP AQLLQDARRH GVEVRPVDVT ASAWDATLED LPADPDAGRR PAVRLGLREI 850 860 870 880 890 900 SGFPEAAALR VAEARAAAPF ADTNDLARRA ALQRRELDLL AAAGALQALA GHRRQAAWQV 910 920 930 940 950 960 AGVCLQGDLF DAAPPPEAAV ALAAPQEAEE LLADYAATGF SLGRHPLALL RARLARGRFL 970 980 990 1000 1010 1020 QAAALARTPD RALVRVAGIV TGRQRPGTAQ GVIFVTLEDE TGSANVVVYA GLAERQRREL 1030 1040 1050 1060 LGARLLGVFG QLQREGEVVH LLAKRLVDLS PWVGGLAARS RDFH

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL96573.1.
RefSeq	YP_935459.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1KCL7.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KCL7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4608210.
GenomeReviews	Gene locus azo3957 in contig AM406670_GR.
KEGG	azo:azo3957.
PATRIC	21016358. VBIAzoSp26047_3996.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	RCANGRR.
ProtClustDB	CLSK768397.
Enzyme and pathway databases
BioCyc	ASP62928:AZO3957-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_AZOSB

Accession

Primary (citable) accession number: A1KCL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program