Reviewed, UniProtKB/Swiss-Prot A1KCI5 (ALR_AZOSB)

Names and origin

Protein names

Recommended name:
    Alanine racemase
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	azo3925

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Alanine racemase HAMAP MF_01201		PRO_1000065969
Sites
	Active site	33	1	Proton acceptor; specific for D-alanine By similarity
	Active site	250	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	33	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KCI5-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 63D5A1311755319C	FASTA	353	37,419
	10 20 30 40 50 60 MRPATALIDL DALRHNYRLA RSRHGGRALA VVKANAYGHG AVRCAQALAA EADGFAVAFL 70 80 90 100 110 120 DEALELRAAG ITQTIVLLEG VFDAAELEQV VAHGLWPVVH HAAQIEMIEQ AALARPLDIW 130 140 150 160 170 180 LKVNSGMNRA GFVGDAVGSA WQRLRASGKV GEITLMTHFA RADEPQVIAT AEQLADFDTA 190 200 210 220 230 240 TRGLPGPRSL ANSAAVLGWP DAHADWARPG ILLYGADPMP GEGNGLQPVM TLESGVIAVR 250 260 270 280 290 300 DLAAGAPLGY GARYVAERPR RIGLVAMGYA DGYPRSAPDG TPVVVDGQPS MLVGRVSMDM 310 320 330 340 350 LTVDLTDLPQ AGIGSRVELW GRQVPINQVA AGAGTIAYEL LCNVKRVVFR YRG

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL96541.1.
RefSeq	YP_935427.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1KCI5.
Genome annotation databases
GeneID	4608579.
GenomeReviews	Gene locus azo3925 in contig AM406670_GR.
KEGG	azo:azo3925.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ARECAVG.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_AZOSB

Accession

Primary (citable) accession number: A1KCI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)