Reviewed, UniProtKB/Swiss-Prot A1KBB4 (URE1_AZOSB)

Names and origin

Protein names

Recommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	azo3504

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity.
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Urease subunit alpha HAMAP MF_01953		PRO_1000073697
Regions
	Domain	130 – 568	439	Urease
Sites
	Active site	321	1	Proton donor By similarity
	Metal binding	135	1	Nickel 2 By similarity
	Metal binding	137	1	Nickel 2 By similarity
	Metal binding	218	1	Nickel 1; via carbamate group By similarity
	Metal binding	218	1	Nickel 2; via carbamate group By similarity
	Metal binding	247	1	Nickel 1 By similarity
	Metal binding	273	1	Nickel 1 By similarity
	Metal binding	361	1	Nickel 2 By similarity
	Binding site	220	1	Substrate By similarity
Amino acid modifications
	Modified residue	218	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KBB4-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 8CCE7D3F3DF1E3D5	FASTA	568	60,295
	10 20 30 40 50 60 MAKITRRAYA EMFGPTTGDK LRLADTELII EVEKDYTIYG EEVKFGGGKV IRDGMGQGQQ 70 80 90 100 110 120 LAAEIADTII TNALIVDAVA GIVKADVGLK DGRIWKIGKG GNPDIQPGVT IPVGAGTEVI 130 140 150 160 170 180 AGEGMILTAG GIDSHIHWIC PQQIDEALMS GVTTMLGGGT GPATGTYATT CTPGPWHIHR 190 200 210 220 230 240 MLEAADAFPM NMGFFGKGNA SLPGPLKEQV EAGVIGLKLH EDWGTTPAAI DNCLTVADEM 250 260 270 280 290 300 DVQVAIHTDT LNESGFVETT LAAFKGRTIH TFHTEGAGGG HAPDIIKAVG QANVLPSSTN 310 320 330 340 350 360 PTRPYTVNTI DEHLDMLMVC HHLDPAIAED VAFAESRIRR ETIAAEDILH DTGAFSMMSS 370 380 390 400 410 420 DSQAMGRVGE VVIRTWQTAH KMKVQRGALA EDAGKGNDNF RIKRYIAKYT INPAITHGIA 430 440 450 460 470 480 HVVGSIEEGK LADLVLWKPA FFGVKPSLIL KGGMIAAAAM GDANASIPTP QPVHYRPMFG 490 500 510 520 530 540 SYGKALKTSV TFVSQAALAN PAVAALQLSK PLVAVQGCRT VQKADMVHNG ATPAIDVDPE 550 560 TYVVRADGEL LVCEPASELP MAQRYFLF

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL96120.1.
RefSeq	YP_935006.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1KBB4.
Protein family/group databases
MEROPS	M38.982.
Genome annotation databases
GeneID	4607979.
GenomeReviews	Gene locus azo3504 in contig AM406670_GR.
KEGG	azo:azo3504.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SHIHFIC.
Family and domain databases
HAMAP	MF_01953. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017952. Urease_asu_core. IPR017950. Urease_asu_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
TIGRFAMs	TIGR01792. urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_AZOSB

Accession

Primary (citable) accession number: A1KBB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)